Protein Alignment pum and PUM1

DIOPT Version :10

• Sequence 1: NP_524285.2
• Gene: pum / 41094
• FlyBaseID: FBgn0003165
• Length: 1533
• Species: Drosophila melanogaster
• Sequence 2: NP_001018494.1
• Gene: PUM1 / 9698
• HGNCID: 14957
• Length: 1188
• Species: Homo sapiens
• Alignment Length: 1392
• Identity: 519/1392 - (37%)
• Similarity: 647/1392 - (46%)
• Gaps: 397/1392 - (28%)

  Fly   219 PHQQHPQQQQQQQQAQNQGHPHHLMGGGNGLGNGNGLGIQHPGQQQQQQQQQQQQQHPGQYNANL 283
            ||.:|     ..|:..|...|..|..|              .|.|.|.|....|....|.:::.:
Human    20 PHLKH-----HPQEPANPNMPVVLTSG--------------TGSQAQPQPAANQALAAGTHSSPV 65

  Fly   284 LNHAAALGHMSSYAQSGGSMYDHHGGAMHPGMNGGMPKQQPLGPPGAGGPQDYVYMGGQTTVPMG 348
            .......|.....|     |.|:.....|....||       |..|.||     |...:...|.|
Human    66 PGSIGVAGRSQDDA-----MVDYFFQRQHGEQLGG-------GGSGGGG-----YNNSKHRWPTG 113

  Fly   349 --------AAMMPPQNQYMNSSAVAAANRNAAITTSTAKKLWEKSDGKGVSSSTPGGPLHPLQIP 405
                    ...|...|.  :..|:|...|.........||.||..:      |:..||      .
Human   114 DNIHAEHQVRSMDELNH--DFQALALEGRAMGEQLLPGKKFWETDE------SSKDGP------K 164

  Fly   406 GI--GDPSSVWKDHTWSTQGENILVPPPSRAYAHGGASDTSNSGNAGILSPRDST---CAKVVEY 465
            ||  ||.   |:|..|.|...:  |..|.......|.|...||....:||||..:   ...:|||
Human   165 GIFLGDQ---WRDSAWGTSDHS--VSQPIMVQRRPGQSFHVNSEVNSVLSPRSESGGLGVSMVEY 224

  Fly   466 VFSGSPTNKDSSLSGLEPHLRNLKFDDNDKSRDDKEKANSPFDTNG-LKKDDQV---TNSNGVVN 526
            |.|.||.:......|..|  |:...|:|||. :.|.|.....|..| ||::..|   ||...|.|
Human   225 VLSSSPGDSCLRKGGFGP--RDADSDENDKG-EKKNKGTFDGDKLGDLKEEGDVMDKTNGLPVQN 286

  Fly   527 GIDDD-KGFNRTPGSRQPSPAEESQPRPPNLLFPPLPFNHMLMDHGQGMGGGLGGVVGSGNGVGG 590
            |||.| |.|:||||:.|.|..|      .:||.|                        :.||..|
Human   287 GIDADVKDFSRTPGNCQNSANE------VDLLGP------------------------NQNGSEG 321

  Fly   591 GSGGGGAGGAYAAHQQMAAQMSQLQPPMMNGVGGGMPMAAQSPMLNHQAAGPNHMESPGNLLQQQ 655
                                ::||     ....|..|:...|.| ..|:...:.||..|  ::..
Human   322 --------------------LAQL-----TSTNGAKPVEDFSNM-ESQSVPLDPMEHVG--MEPL 358

  Fly   656 NFDVQQLFRSQNPGLAAVATNAAAAAAAAAAATSAASAAAAVGAPPVPNGSLQQSQQQQQQQQQQ 720
            .||.                        :.......||||.||.               .....|
Human   359 QFDY------------------------SGTQVPVDSAAATVGL---------------FDYNSQ 384

  Fly   721 QQQQQMHMAAASQQFLAAQQQAQNAAYAAQQ-----------ATSYVIN---PGQEAAPY-MGMI 770
            ||..|...|.|.||..||||| |.|..||.|           ...|:|:   ||.:  || .|:.
Human   385 QQLFQRPNALAVQQLTAAQQQ-QYALAAAHQPHIGLAPAAFVPNPYIISAAPPGTD--PYTAGLA 446

  Fly   771 AAAQM-------PYYGVAPWGMYPGNLIPQQ----------------------------GTQPRR 800
            |||.:       .||||.|||:||.:|..||                            |...:|
Human   447 AAATLGPAVVPHQYYGVTPWGVYPASLFQQQAAAAAAATNSANQQTTPQAQQGQQQVLRGGASQR 511

  Fly   801 PLTP--SQQGAENQP-----------------------YQVI--PAFLDHTGSLLM-GGPRT--G 835
            ||||  :|||.:..|                       |.|:  .|:.|.||:|:: .|.|.  |
Human   512 PLTPNQNQQGQQTDPLVAAAAVNSALAFGQGLAAGMPGYPVLAPAAYYDQTGALVVNAGARNGLG 576

  Fly   836 TPMRLVSPAPVLVPPGATRA---------------------GPPPPQGPQLYQPQPQTAQQNLYS 879
            .|:|||:||||::...|.:|                     ||..|.|.|  |||||..||...:
Human   577 APVRLVAPAPVIISSSAAQAAVAAAAASANGAAGGLAGTTNGPFRPLGTQ--QPQPQPQQQPNNN 639

  Fly   880 QQNGSSVGGLALNTSSLTGRRDSFDRSTSAFSPSTMDYTSSGVA-AAANAVNSTVAQAAAAAAAA 943
            ..:.|..|..:||::|         :|:|.||..:....::.:. .:::::.:|:..|......|
Human   640 LASSSFYGNNSLNSNS---------QSSSLFSQGSAQPANTSLGFGSSSSLGATLGSALGGFGTA 695

  Fly   944 AAARGKWPGAMSGAASGA------------------YGALGAGNASASPLGAPI---------TP 981
            .|......|:...:.:|:                  |..| :.::|..|:|.|:         ||
Human   696 VANSNTGSGSRRDSLTGSSDLYKRTSSSLTPIGHSFYNGL-SFSSSPGPVGMPLPSQGPGHSQTP 759

  Fly   982 PP------SAQSCLLG----------SRAPGAESRQRQQQQQQLAAVGLPATAAAAQAAVAAAAN 1030
            ||      |:.|..||          |.|||||::.|.                      |::|:
Human   760 PPSLSSHGSSSSLNLGGLTNGSGRYISAAPGAEAKYRS----------------------ASSAS 802

  Fly  1031 NMFGSNSSIFSNPLAIPGTAAVAAAAAAAAAANSRQVAATAAAAAAVAAAAGGVGGAPQPGRSRL 1095
            ::|..:|::||:                                   :....|:......|||||
Human   803 SLFSPSSTLFSS-----------------------------------SRLRYGMSDVMPSGRSRL 832

  Fly  1096 LEDFRNQRYPNLQLRDLANHIVEFSQDQHGSRFIQQKLERATAAEKQMVFSEILAAAYSLMTDVF 1160
            ||||||.||||||||::|.||:|||||||||||||.||||||.||:|:||:|||.|||.||.|||
Human   833 LEDFRNNRYPNLQLREIAGHIMEFSQDQHGSRFIQLKLERATPAERQLVFNEILQAAYQLMVDVF 897

  Fly  1161 GNYVIQKFFEFGTPEQKNTLGMQVKGHVLQLALQMYGCRVIQKALESISPEQQ--QEIVHELDGH 1223
            ||||||||||||:.|||..|..:::||||.||||||||||||||||.|..:||  .|:|.|||||
Human   898 GNYVIQKFFEFGSLEQKLALAERIRGHVLSLALQMYGCRVIQKALEFIPSDQQVINEMVRELDGH 962

  Fly  1224 VLKCVKDQNGNHVVQKCIECVDPVALQFIINAFKGQVYSLSTHPYGCRVIQRILEHCTAEQTTPI 1288
            |||||||||||||||||||||.|.:|||||:||||||::||||||||||||||||||..:||.||
Human   963 VLKCVKDQNGNHVVQKCIECVQPQSLQFIIDAFKGQVFALSTHPYGCRVIQRILEHCLPDQTLPI 1027

  Fly  1289 LDELHEHTEQLIQDQYGNYVIQHVLEHGKQEDKSILINSVRGKVLVLSQHKFASNVVEKCVTHAT 1353
            |:|||:|||||:||||||||||||||||:.||||.::..:||.|||||||||||||||||||||:
Human  1028 LEELHQHTEQLVQDQYGNYVIQHVLEHGRPEDKSKIVAEIRGNVLVLSQHKFASNVVEKCVTHAS 1092

  Fly  1354 RGERTGLIDEVCTFND---NALHVMMKDQYANYVVQKMIDVSEPTQLKKLMTKIRPHMAALRKYT 1415
            |.||..|||||||.||   :||:.|||||||||||||||||:||.|.|.:|.|||||:|.|||||
Human  1093 RTERAVLIDEVCTMNDGPHSALYTMMKDQYANYVVQKMIDVAEPGQRKIVMHKIRPHIATLRKYT 1157

  Fly  1416 YGKHINAKLEKYYMKITNPITVGTGAG 1442
            |||||.|||||||||  |.:.:|...|
Human  1158 YGKHILAKLEKYYMK--NGVDLGPICG 1182

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
pum	NP_524285.2	Pumilio	1106..1424	CDD:153420	249/322 (77%)
PUM1	NP_001018494.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	22..73		12/69 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	233..272		13/41 (32%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	485..524		9/38 (24%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	613..648		13/36 (36%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	742..773		9/30 (30%)
		Pumilio	843..1166	CDD:153420	249/322 (77%)
		Pumilio 1. /evidence=ECO:0000255|PROSITE-ProRule:PRU00317, ECO:0000269|PubMed:21397187	848..883		26/34 (76%)
		Adenine-nucleotide binding in RNA target. /evidence=ECO:0000269|PubMed:18328718	863..867		3/3 (100%)
		Pumilio 2. /evidence=ECO:0000255|PROSITE-ProRule:PRU00317, ECO:0000269|PubMed:21397187	884..919		27/34 (79%)
		Uracil-nucleotide binding in RNA target. /evidence=ECO:0000269|PubMed:18328718	899..903		3/3 (100%)
		Adenine-nucleotide binding in RNA target. /evidence=ECO:0000269|PubMed:18328718	935..939		3/3 (100%)
		Pumilio 4. /evidence=ECO:0000255|PROSITE-ProRule:PRU00317, ECO:0000269|PubMed:21397187	958..993		31/34 (91%)
		Non-specific-nucleotide binding in RNA target. /evidence=ECO:0000269|PubMed:18328718	973..977		3/3 (100%)
		Pumilio 5. /evidence=ECO:0000255|PROSITE-ProRule:PRU00317, ECO:0000269|PubMed:21397187	994..1029		28/34 (82%)
		Adenine-nucleotide binding in RNA target. /evidence=ECO:0000269|PubMed:18328718	1009..1013		3/3 (100%)
		Pumilio 6. /evidence=ECO:0000255|PROSITE-ProRule:PRU00317, ECO:0000269|PubMed:21397187	1030..1065		28/34 (82%)
		Uracil-nucleotide binding in RNA target. /evidence=ECO:0000269|PubMed:18328718, ECO:0000269|PubMed:21397187, ECO:0007744|PDB:3BSB	1045..1049		3/3 (100%)
		Pumilio 7. /evidence=ECO:0000255|PROSITE-ProRule:PRU00317, ECO:0000269|PubMed:21397187	1066..1101		27/34 (79%)
		Guanine-nucleotide binding in RNA target. /evidence=ECO:0000269|PubMed:18328718, ECO:0000269|PubMed:21397187, ECO:0007744|PDB:3BSB	1081..1085		3/3 (100%)
		Pumilio 8. /evidence=ECO:0000255|PROSITE-ProRule:PRU00317, ECO:0000269|PubMed:21397187	1105..1144		26/38 (68%)
		Uracil-nucleotide binding in RNA target. /evidence=ECO:0000269|PubMed:18328718, ECO:0000269|PubMed:21397187, ECO:0007744|PDB:1M8W, ECO:0007744|PDB:1M8X, ECO:0007744|PDB:3BSB, ECO:0007744|PDB:3BSX, ECO:0007744|PDB:3Q0L, ECO:0007744|PDB:3Q0M, ECO:0007744|PDB:3Q0N, ECO:0007744|PDB:3Q0O, ECO:0007744|PDB:3Q0P	1124..1128		3/3 (100%)