Protein Alignment pum and PUF4

DIOPT Version :10

• Sequence 1: NP_524285.2
• Gene: pum / 41094
• FlyBaseID: FBgn0003165
• Length: 1533
• Species: Drosophila melanogaster
• Sequence 2: NP_011501.1
• Gene: PUF4 / 852870
• SGDID: S000002982
• Length: 888
• Species: Saccharomyces cerevisiae
• Alignment Length: 1087
• Identity: 230/1087 - (21%)
• Similarity: 377/1087 - (34%)
• Gaps: 362/1087 - (33%)

  Fly   486 RNLKFDDNDKSRDD---KEKANSPFDTNGLKKDDQVTNSNGVVNGIDDDKGF-NRTPGSRQPSP- 545
            :.||.:.:|....|   .|..||..:...|...::...||...|.:..|..| |..|....|.|
Yeast     4 KGLKEEIDDVPSVDPVVSETVNSALEQLQLDDPEENATSNAFANKVSQDSQFANGPPSQMFPHPQ 68

  Fly   546 ----------AEESQ-PRPPNLLFPPLPFNHMLMDHGQGMGG---GLGG-------------VVG 583
                      ::..| |..|...|||..||    |....:..   ..||             ::.
Yeast    69 MMGGMGFMPYSQMMQVPHNPCPFFPPPDFN----DPTAPLSSSPLNAGGPPMLFKNDSLPFQMLS 129

  Fly   584 SGNGVGGGSGGGGAGGAYAAHQQMAAQMSQLQPPMMNGVG-GGMPMAAQSPMLNH---------- 637
            ||..|                   |.|..|...|::|... ..:|:|:..|:..|
Yeast   130 SGAAV-------------------ATQGGQNLNPLINDNSMKVLPIASADPLWTHSNVPGSASVA 175

  Fly   638 --------QAAGPN-----------------HMESPGNLLQQQN---------FDVQQLFRSQNP 668
                    |.:.|:                 |..||.:|:...|         .|:|...:::.|
Yeast   176 IEETTATLQESLPSKGRESNNKASSFRRQTFHALSPTDLINAANNVTLSKDFQSDMQNFSKAKKP 240

  Fly   669 GLAAVATNAAAAAAAAAAATSAASAAAAVGAPPVPNGSLQQSQQQQQQQQQQQQQQQMHMAAASQ 733
            .:.  |.|.|.....:.:..:..|:.:.:  ||..:    .|::....:.:..::..::..|.::
Yeast   241 SVG--ANNTAKTRTQSISFDNTPSSTSFI--PPTNS----VSEKLSDFKIETSKEDLINKTAPAK 297

  Fly   734 QFLAAQQQAQNAAYAAQQATSYVINPGQEAAPYMGMIAAAQMPYYG--VAPWGMYPGNLIPQQGT 796
            :               :..|:|                .|..||.|  :.|..:.||:  |...:
Yeast   298 K---------------ESPTTY----------------GAAYPYGGPLLQPNPIMPGH--PHNIS 329

  Fly   797 QP----RRPLTPS-QQGAENQPYQVIPAFLDHTGSLLMGGPRTGTPMRLVSPAPVLVPPGAT--- 853
            .|    |.|...| :.||:.||:..|.....|:.:.....|.|.:|:.|   ||||.|...:
Yeast   330 SPIYGIRSPFPNSYEMGAQFQPFSPILNPTSHSLNANSPIPLTQSPIHL---APVLNPSSNSVAF 391

  Fly   854 ---------------RAGP---------------------------PPPQGPQLYQ-PQPQTAQ- 874
                           :|||                           |||  |.||. |.|..|.
Yeast   392 SDMKNDGGKPTTDNDKAGPNVRMDLINPNLGPSMQPFHILPPQQNTPPP--PWLYSTPPPFNAMV 454

  Fly   875 ------QNLYSQQNGSSVGGLALNTSSLTGRRDSFDRSTSAFSPSTMDYTSSGVAAAANAVNSTV 933
                  ||.....|.::......|.:|::...|:.:...|.:  :..|...|.|....|..||
Yeast   455 PPHLLAQNHMPLMNSANNKHHGRNNNSMSSHNDNDNIGNSNY--NNKDTGRSNVGKMKNMKNS-- 515

  Fly   934 AQAAAAAAAAAAARGKWPGAMSGAASGAYGALGAGNASASPLGAPITPPPSAQSCLLGSRAPGAE 998
                                       .:|.....|
Yeast   516 ---------------------------YHGYYNNNN----------------------------- 524

  Fly   999 SRQRQQQQQQLAAVGLPATAAAAQAAVAAAANNMFGSNSSIFSNPLAIPGTAAVAAAAAAAAAAN 1063
                                           ||...:|::..||                |..:|
Yeast   525 -------------------------------NNNNNNNNNNNSN----------------ATNSN 542

  Fly  1064 SRQVAATAAAAAAVAAAAGGVGGAPQPGRSRLLEDFRNQRYPNLQLRDLANHIVEFSQDQHGSRF 1128
            |.:                         :.|.:|:  :.|:.:..|......|....:||||.||
Yeast   543 SAE-------------------------KQRKIEE--SSRFADAVLDQYIGSIHSLCKDQHGCRF 580

  Fly  1129 IQQKLERATAAEKQMVFSEILAAAYSLMTDVFGNYVIQKFFEFGTPEQKNTLGMQVKGHVLQLAL 1193
            :|::|:...:.....:|.|.......||||.||||:|||..|..|.||:..|......|.::::|
Yeast   581 LQKQLDILGSKAADAIFEETKDYTVELMTDSFGNYLIQKLLEEVTTEQRIVLTKISSPHFVEISL 645

  Fly  1194 QMYGCRVIQKALESI-SPEQQQEIVHELDGHVLKCVKDQNGNHVVQKCIECVDPVALQFIINAFK 1257
            ..:|.|.:||.:|.| :.|:.|.:|..|..:.::..||.|||||:|||::.:.|...|||.:|..
Yeast   646 NPHGTRALQKLIECIKTDEEAQIVVDSLRPYTVQLSKDLNGNHVIQKCLQRLKPENFQFIFDAIS 710

  Fly  1258 GQVYSLSTHPYGCRVIQRILEHCTAEQTTPILDELHEHTEQLIQDQYGNYVIQHVL----EHGKQ 1318
            .....::||.:||.|:||.|:|.|.||...:.|:|....::|..|.:||||:|:::    |..|.
Yeast   711 DSCIDIATHRHGCCVLQRCLDHGTTEQCDNLCDKLLALVDKLTLDPFGNYVVQYIITKEAEKNKY 775

  Fly  1319 EDKSILINSVRGKVLVLSQHKFASNVVEKCVTHATRGERTGLIDEVCTF------NDNALHVMMK 1377
            :....:::.::.:.:.||.|||.|||:||.:       :|.::.|....      .:..:..::.
Yeast   776 DYTHKIVHLLKPRAIELSIHKFGSNVIEKIL-------KTAIVSEPMILEILNNGGETGIQSLLN 833

  Fly  1378 DQYANYVVQKMIDVSEPTQ---LKKLMTKIRPHMAA-LRKYTYGKHI 1420
            |.|.|||:|..:|:|....   .|:|...:.|.:.. :|...:||.|
Yeast   834 DSYGNYVLQTALDISHKQNDYLYKRLSEIVAPLLVGPIRNTPHGKRI 880

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
pum	NP_524285.2	Pumilio	1106..1424	CDD:153420	105/330 (32%)
PUF4	NP_011501.1	COG5099	65..888	CDD:227430	215/1026 (21%)