Protein Alignment pum and PUF3

DIOPT Version :10

• Sequence 1: NP_524285.2
• Gene: pum / 41094
• FlyBaseID: FBgn0003165
• Length: 1533
• Species: Drosophila melanogaster
• Sequence 2: NP_013088.1
• Gene: PUF3 / 850647
• SGDID: S000003936
• Length: 879
• Species: Saccharomyces cerevisiae
• Alignment Length: 1266
• Identity: 298/1266 - (23%)
• Similarity: 442/1266 - (34%)
• Gaps: 516/1266 - (40%)

  Fly   281 ANLLNHAAALGHMSSYAQSGGSMYDHHGGAMHPGMNGGMPKQQPLGPPGAGGPQDYVYMGG---- 341
            |::::..:||.|.::                    |||......:...||.|.|.   :||
Yeast    14 ASIVSSLSALSHSNN--------------------NGGQAAAAGIVNGGAAGSQQ---IGGFRRS 55

  Fly   342 --------------------------QTTVPMGAAMMPP----------------QNQYMNSSAV 364
                                      :|.:.:|.|  ||                |.:..:::::
Yeast    56 SFTTANEVDSEILLLHGSSESSPIFKKTALSVGTA--PPFSTNSKKFFGNGGNYYQYRSTDTASL 118

  Fly   365 AAANRNAAITTSTAKKLWEKSDGK--------GVSSSTPGGPLHPLQIPGIGDPSSVWKDHTWST 421
            ::|:.|...|..||..|     ||        |..|::..||:
Yeast   119 SSASYNNYHTHHTAANL-----GKNNKVNHLLGQYSASIAGPV---------------------- 156

  Fly   422 QGENILVPPPSRAYAHGGASDTSNSGNAGILSPRDSTCAKVVEYVFSGSPTNKDSSLSGLEPHLR 486
                         |.:|  :|.:|||..|...       |..:.:..|:               |
Yeast   157 -------------YYNG--NDNNNSGGEGFFE-------KFGKSLIDGT---------------R 184

  Fly   487 NLKFDDNDKSRDDKEKANSPFDTNGLKKDDQVTNSNGVVNGIDDDKGFNR--------------T 537
            .|:..|    |.|.....|.|.:..: .:..:...|.....::.||.||:              :
Yeast   185 ELESQD----RPDAVNTQSQFISKSV-SNASLDTQNTFEQNVESDKNFNKLNRNTTNSGSLYHSS 244

  Fly   538 PGSRQPSPAEESQPRPP-----NLLFPPL--PFNHMLMDHGQGMGGGLGGVVGSGNGVGGGSGGG 595
            ..|...:..|......|     |:...|:  |.|:             ...||:.|........|
Yeast   245 SNSGSSASLESENAHYPKRNIWNVANTPVFRPSNN-------------PAAVGATNVALPNQQDG 296

  Fly   596 GAGGAYAAHQQMAAQMSQLQPPMMNGVGGGMPMAAQSPMLNHQAAGPNHMESPGNLLQQQNFDVQ 660
            .|...:              ||.|||.         .|...||  ||::...|..|:...:..:.
Yeast   297 PANNNF--------------PPYMNGF---------PPNQFHQ--GPHYQNFPNYLIGSPSNFIS 336

  Fly   661 QLFRSQNPGLAAVATNAAAAAAAAAAATSAASAAA--------------AVGAPPVPNGSLQQSQ 711
            |:...|.|.......:.......|...:|.:|.::              .:..||.|..:.||.|
Yeast   337 QMISVQIPANEDTEDSNGKKKKKANRPSSVSSPSSPPNNSPFPFAYPNPMMFMPPPPLSAPQQQQ 401

  Fly   712 QQQQQQQQQQQQQQMHMAAASQQFLAAQQQAQNAAYAAQQATSYVINPGQEAAPYMGMIAAAQMP 776
            ||||||||:.|||              |||.:|                    ||:
Yeast   402 QQQQQQQQEDQQQ--------------QQQQEN--------------------PYI--------- 423

  Fly   777 YYGVAPWGMYPGNLIPQQGTQPRRPLTPSQQGAENQPYQVIPAFLDHTGSLLMGGPRTGTPMRLV 841
            ||...       |.||.:  .|:...|..::..:|.|
Yeast   424 YYPTP-------NPIPVK--MPKDEKTFKKRNNKNHP---------------------------- 451

  Fly   842 SPAPVLVPPGATRAGPPPPQ-GPQLYQ--PQPQTAQQNLYSQQNGSSVGGLALNTSSLTGRRDSF 903
                      |..:.....| .|.|..  |...|:::|..|:.|.|:......::.|
Yeast   452 ----------ANNSNNANKQANPYLENSIPTKNTSKKNASSKSNESTANNHKSHSHS-------- 498

  Fly   904 DRSTSAFSPSTMDYTSSGVAAAANAVNSTVAQAAAAAAAAAAARGKWPGAMSGAASGAYGALGAG 968
                                                                             
Yeast   499 ----------------------------------------------------------------- 498

  Fly   969 NASASPLGAPITPPPSAQSCLLGSRAPGAESRQRQQQQQQLAAVGLPATAAAAQAAVAAAANNMF 1033
                                     .|.::|.|:|||...
Yeast   499 -------------------------HPHSQSLQQQQQTYH------------------------- 513

  Fly  1034 GSNSSIFSNPLAIPGTAAVAAAAAAAAAANSRQVAATAAAAAAVAAAAGGVGGAPQPGRSRLLED 1098
                                                                      ||.|||.
Yeast   514 ----------------------------------------------------------RSPLLEQ 520

  Fly  1099 FRN-----QRYPNLQLRDLANHIVEFSQDQHGSRFIQQKLERATAAEKQMVFSEILAAAYSLMTD 1158
            .||     ....|:.|:|:..|.:||.:|||||||||::|..:.|:||:::|:||...|..|..|
Yeast   521 LRNSSSDKNSNSNMSLKDIFGHSLEFCKDQHGSRFIQRELATSPASEKEVIFNEIRDDAIELSND 585

  Fly  1159 VFGNYVIQKFFEFGTPEQKNTLGMQVKGHVLQLALQMYGCRVIQKALESISPEQQQEIVHELDGH 1223
            ||||||||||||||:..|||||..|.||::.||:||||.|||||||||.|...|:.|:|.||...
Yeast   586 VFGNYVIQKFFEFGSKIQKNTLVDQFKGNMKQLSLQMYACRVIQKALEYIDSNQRIELVLELSDS 650

  Fly  1224 VLKCVKDQNGNHVVQKCIECVDPVALQFIINAFKGQVYSLSTHPYGCRVIQRILEHCTAEQTTPI 1288
            ||:.:||||||||:||.||.:....|.||:::..|.:|.||||.||||||||:||..::|....|
Yeast   651 VLQMIKDQNGNHVIQKAIETIPIEKLPFILSSLTGHIYHLSTHSYGCRVIQRLLEFGSSEDQESI 715

  Fly  1289 LDELHEHTEQLIQDQYGNYVIQHVLEHGKQEDKSI------LINSVRGKVLVLSQHKFASNVVEK 1347
            |:||.:....||||||||||||:||:..:..:|.:      :|.:|...|:..|:||||||||||
Yeast   716 LNELKDFIPYLIQDQYGNYVIQYVLQQDQFTNKEMVDIKQEIIETVANNVVEYSKHKFASNVVEK 780

  Fly  1348 CVTHATRGERTGLIDEVC---------TFNDNALHVMMKDQYANYVVQKMIDVSEPTQLKKLMTK 1403
            .:.:.::.::..:|.::.         ..:|:.:.:|:|||:||||:||:::|||....|.::..
Yeast   781 SILYGSKNQKDLIISKILPRDKNHALNLEDDSPMILMIKDQFANYVIQKLVNVSEGEGKKLIVIA 845

  Fly  1404 IRPHMAALRK-YTYGKHINAKLEKYYMKITN 1433
            ||.::..|.| .:.|....|.:||....:.|
Yeast   846 IRAYLDKLNKSNSLGNRHLASVEKLAALVEN 876

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
pum	NP_524285.2	Pumilio	1106..1424	CDD:153420	157/333 (47%)
PUF3	NP_013088.1	COG5099	54..876	CDD:227430	284/1201 (24%)