DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment pum and Pum2

DIOPT Version :9

Sequence 1:	NP_001262403.1	Gene:	pum / 41094	FlyBaseID:	FBgn0003165	Length:	1533	Species:	Drosophila melanogaster
Sequence 2:	NP_001153691.1	Gene:	Pum2 / 80913	MGIID:	1931751	Length:	1066	Species:	Mus musculus

Alignment Length:	1252	Identity:	502/1252 - (40%)
Similarity:	617/1252 - (49%)	Gaps:	365/1252 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly   363 AVAAANRNAAITTSTAKKLWEKSDGKGVSSSTPGGPLHPLQIPGI--GDPSSVWKDHTWSTQGEN 425
            |:|..:|.......| ||.||..|      ||..|.      .||  ||..  |::..|.|...:
Mouse     7 ALALESRGMGELLPT-KKFWEPDD------STKDGQ------KGIFLGDDE--WRETAWGTSHHS 56

  Fly   426 ILVPPPSRAYAHGGASDTSNSGNAGILSPRDST---CAKVVEYVFSGSPTNKDSSLSGLEPHLRN 487
              :..|.......|.|...||....|||||..:   ...:||||.|.||.:|      |:...|.
Mouse    57 --MSQPIMVQRRSGQSFHGNSEVNAILSPRSESGGLGVSMVEYVLSSSPADK------LDSRFRK 113

  Fly   488 LKFDDNDKSRDDKEKAN-----SPFD---TNGLKKDDQVT--NSNGVVNGIDDD-KGFNRTPGSR 541
            ..|...|...|..||.:     |||:   ...||:||:.:  |..|:.||:|.| |.||||||||
Mouse   114 GTFGTRDAETDGPEKGDQKGKASPFEEDQNRDLKQDDEDSKINGRGLPNGMDADCKDFNRTPGSR 178

  Fly   542 QPSPAEESQPRPPNLLFPPLPFNHMLMDHGQGMGGGLGGVVGSGNGVGGGSGGGGAGGAYAAHQQ 606
            |.||.|..:...|: ..||           :|:|
Mouse   179 QASPTEVVERLGPS-TNPP-----------EGLG------------------------------- 200

  Fly   607 MAAQMSQLQPPMMNGVGGGMPMAAQSPML----NHQAAGPNHMESPGNLLQQQNFDVQQLFRSQN 667
                      |:.|       ..|..|::    |.:....:.|:..|  |....||.
Mouse   201 ----------PLPN-------PTANKPLVEEFSNPETQNLDAMDQVG--LDSLQFDY-------- 238

  Fly   668 PGLAAVATNAAAAAAAAAAATSAASAAAAVGAPPVPNGSLQQSQQQQQ----------QQQQQQQ 722
            ||                ......|:.|.||        |.....|||          ||....|
Mouse   239 PG----------------NQVPMDSSGATVG--------LFDYNSQQQLFQRTSALTVQQLTAAQ 279

  Fly   723 QQQMHMAAASQQFLAAQQQAQNAAYAAQQATSYVIN---PGQEAAPYMGMIAAAQM--------P 776
            |||..:|||.|..:|....| ..|.||.....|:|:   ||.:.....|:.|||.:        .
Mouse   280 QQQYALAAAQQPHIAGVFSA-GLAPAAFVPNPYIISAAPPGTDPYTAAGLAAAATLAGPAVVPPQ 343

  Fly   777 YYGVAPWGMYPGNLIPQQG---------------TQP-----------RRPLTPS--QQG----- 808
            |||| |||:||.||..||.               .||           :||:|||  |||
Mouse   344 YYGV-PWGVYPANLFQQQAAAAASNTANQQAASQAQPGQQQVLRPGAGQRPITPSQGQQGQQAES 407

  Fly   809 ---------------AENQP-YQVI--PAFLDHTGSLLMG-GPRT--GTPMRLVSPAPVLVPPGA 852
                           |...| |||:  .|:.|.||:|::| |.||  |.|:||::|.|||:...|
Mouse   408 LAAAANPTLAFGQSLAAGMPGYQVLAPTAYYDQTGALVVGPGARTGLGAPVRLMAPTPVLISSTA 472

  Fly   853 TRA-------------------------GPPPPQGPQLYQPQPQTAQQNLYSQQNGSSVGGLALN 892
            .:|                         |..||| .|..|.||.|   ||   |:.|..|..:|.
Mouse   473 AQAAAAAAAAGGTANSLTGSTNGLFRPIGTQPPQ-QQQQQQQPST---NL---QSNSFYGSSSLT 530

  Fly   893 TSSLTGRRDSFDRSTSAFS-----PSTMDY---TSSGVAAAANAVNSTVAQAAAAAAAAAAAR-- 947
            .||         :|:|.||     |.:...   :.|.:.||..:..|....:..::|:::|.|
Mouse   531 NSS---------QSSSLFSHGPGQPGSASLGFGSGSSLGAAIGSALSGFGSSVGSSASSSATRRE 586

  Fly   948 ---------GKWPGAMSGAASGAYGALGAGNASASPLGAP---------ITPPP------SAQSC 988
                     .:...:::......|.:||. ::|.||:|.|         :||||      |:.|.
Mouse   587 SLSTSSDLYKRSSSSLAPIGQPFYNSLGF-SSSPSPIGMPLPSQTPGHSLTPPPSLSSHGSSSSL 650

  Fly   989 LLG----------SRAPGAESRQRQQQQQQLAAVGLPATAAAAQAAVAAAANNMFGSNSSIFSNP 1043
            .||          |.|||||::.|.                      |::.:::|.|:|.:|
Mouse   651 HLGGLTNGSGRYISAAPGAEAKYRS----------------------ASSTSSLFSSSSQLF--- 690

  Fly  1044 LAIPGTAAVAAAAAAAAAANSRQVAATAAAAAAVAAAAGGVGGAPQPGRSRLLEDFRNQRYPNLQ 1108
                        ..:....|...:..:                    |||||||||||.|:||||
Mouse   691 ------------PPSRLRYNRSDIMPS--------------------GRSRLLEDFRNNRFPNLQ 723

  Fly  1109 LRDLANHIVEFSQDQHGSRFIQQKLERATAAEKQMVFSEILAAAYSLMTDVFGNYVIQKFFEFGT 1173
            ||||..|||||||||||||||||||||||.||:|:||:|||.|||.||||||||||||||||||:
Mouse   724 LRDLIGHIVEFSQDQHGSRFIQQKLERATPAERQIVFNEILQAAYQLMTDVFGNYVIQKFFEFGS 788

  Fly  1174 PEQKNTLGMQVKGHVLQLALQMYGCRVIQKALESISPEQQ--QEIVHELDGHVLKCVKDQNGNHV 1236
            .:||..|..:::||||.|||||||||||||||||||.:||  .|:|.||||||||||||||||||
Mouse   789 LDQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQVISEMVKELDGHVLKCVKDQNGNHV 853

  Fly  1237 VQKCIECVDPVALQFIINAFKGQVYSLSTHPYGCRVIQRILEHCTAEQTTPILDELHEHTEQLIQ 1301
            ||||||||.|.:|||||:||||||:.|||||||||||||||||||||||.|||:|||:|||||:|
Mouse   854 VQKCIECVQPQSLQFIIDAFKGQVFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQHTEQLVQ 918

  Fly  1302 DQYGNYVIQHVLEHGKQEDKSILINSVRGKVLVLSQHKFASNVVEKCVTHATRGERTGLIDEVCT 1366
            |||||||||||||||:.||||.:::.:|||||.||||||||||||||||||:|.||..||||||.
Mouse   919 DQYGNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVVEKCVTHASRAERALLIDEVCC 983

  Fly  1367 FND---NALHVMMKDQYANYVVQKMIDVSEPTQLKKLMTKIRPHMAALRKYTYGKHINAKLEKYY 1428
            .||   :||:.||||||||||||||||::||.|.|.:|.|||||:..||||||||||.|||||||
Mouse   984 QNDGPHSALYTMMKDQYANYVVQKMIDMAEPAQRKIIMHKIRPHITTLRKYTYGKHILAKLEKYY 1048

  Fly  1429 MKITNPITVGTGAGGVP 1445
            :|  |...:|. .||.|
Mouse  1049 LK--NSPDLGP-IGGPP 1062

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
pum	NP_001262403.1	Pumilio	1106..1424	CDD:153420	254/322 (79%)
Pum2	NP_001153691.1	Interaction with SNAPIN. /evidence=ECO:0000250	1..260		92/369 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	106..203		40/155 (26%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	368..408		10/39 (26%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	490..551		22/76 (29%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	620..650		9/29 (31%)
		Pumilio	721..1044	CDD:153420	254/322 (79%)
		Pumilio 1	726..761		29/34 (85%)
		Adenine-nucleotide binding in RNA target. /evidence=ECO:0000250\|UniProtKB:Q14671	741..745		3/3 (100%)
		Pumilio 2	762..797		27/34 (79%)
		Uracil-nucleotide binding in RNA target. /evidence=ECO:0000250\|UniProtKB:Q14671	777..781		3/3 (100%)
		Pumilio 3	798..835		26/36 (72%)
		Adenine-nucleotide binding in RNA target. /evidence=ECO:0000250\|UniProtKB:Q14671	813..817		3/3 (100%)
		Pumilio 4	836..871		31/34 (91%)
		Non-specific-nucleotide binding in RNA target. /evidence=ECO:0000250\|UniProtKB:Q14671	851..855		3/3 (100%)
		Pumilio 5	872..907		31/34 (91%)
		Adenine-nucleotide binding in RNA target. /evidence=ECO:0000250\|UniProtKB:Q14671	887..891		3/3 (100%)
		Pumilio 6	908..943		28/34 (82%)
		Uracil-nucleotide binding in RNA target. /evidence=ECO:0000250\|UniProtKB:Q14671	923..927		3/3 (100%)
		Pumilio 7	944..979		27/34 (79%)
		Guanine-nucleotide binding in RNA target. /evidence=ECO:0000250\|UniProtKB:Q14671	959..963		3/3 (100%)
		Pumilio 8	980..1022		27/41 (66%)
		Uracil-nucleotide binding in RNA target. /evidence=ECO:0000250\|UniProtKB:Q14671	1002..1006		3/3 (100%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167838241
Domainoid	1	1.000	135	1.000	Domainoid score	I4954
eggNOG	1	0.900	-	-		E1_COG5099
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	641	1.000	Inparanoid score	I811
Isobase	1	0.950	-	0	Normalized mean entropy	S1682
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000719
OrthoInspector	1	1.000	-	-		otm42442
orthoMCL	1	0.900	-	-		OOG6_100436
Panther	1	1.100	-	-	LDO	PTHR12537
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1032
SonicParanoid	1	1.000	-	-		X849
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	13	12.820