DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment pum and PUM2

DIOPT Version :9

Sequence 1:	NP_001262403.1	Gene:	pum / 41094	FlyBaseID:	FBgn0003165	Length:	1533	Species:	Drosophila melanogaster
Sequence 2:	NP_001339846.1	Gene:	PUM2 / 23369	HGNCID:	14958	Length:	1066	Species:	Homo sapiens

Alignment Length:	1255	Identity:	505/1255 - (40%)
Similarity:	617/1255 - (49%)	Gaps:	371/1255 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly   363 AVAAANRNAAITTSTAKKLWEKSDGKGVSSSTPGGPLHPLQIPGI--GDPSSVWKDHTWS----T 421
            |:|..:|.......| ||.||..|      ||..|.      .||  ||..  |::..|.    :
Human     7 ALALESRGMGELLPT-KKFWEPDD------STKDGQ------KGIFLGDDE--WRETAWGASHHS 56

  Fly   422 QGENILVPPPSRAYAHGGASDTSNSGNAGILSPRDST---CAKVVEYVFSGSPTNKDSSLSGLEP 483
            ..:.|:|...|....||      ||....|||||..:   ...:||||.|.||.:|      |:.
Human    57 MSQPIMVQRRSGQGFHG------NSEVNAILSPRSESGGLGVSMVEYVLSSSPADK------LDS 109

  Fly   484 HLRNLKFDDNDKSRDDKEKAN-----SPFD---TNGLKK---DDQVTNSNGVVNGIDDD-KGFNR 536
            ..|...|...|...|..||.:     |||:   ...||:   ||...|..|:.||:|.| |.|||
Human   110 RFRKGNFGTRDAETDGPEKGDQKGKASPFEEDQNRDLKQGDDDDSKINGRGLPNGMDADCKDFNR 174

  Fly   537 TPGSRQPSPAEESQPRPPNLLFPPLPFNHMLMDHGQGMGGGLGGVVGSGNGVGGGSGGGGAGGAY 601
            ||||||.||.|..:...||            .:..:|:|
Human   175 TPGSRQASPTEVVERLGPN------------TNPSEGLG-------------------------- 201

  Fly   602 AAHQQMAAQMSQLQPPMMNGVGGGMPMAAQSPML----NHQAAGPNHMESPGNLLQQQNFDVQQL 662
                           |:.|       ..|..|::    |.:....:.||..|  |:...||.
Human   202 ---------------PLPN-------PTANKPLVEEFSNPETQNLDAMEQVG--LESLQFDY--- 239

  Fly   663 FRSQNPGLAAVATNAAAAAAAAAAATSAASAAAAVGAPPVPNGSLQQSQQQQQ----------QQ 717
                 ||                ......|:.|.||        |.....|||          ||
Human   240 -----PG----------------NQVPMDSSGATVG--------LFDYNSQQQLFQRTNALTVQQ 275

  Fly   718 QQQQQQQQMHMAAASQQFLAAQQQAQNAAYAAQQATSYVIN---PGQEAAPYMGMIAAAQM---- 775
            ....||||..:|||.|..:|....| ..|.||.....|:|:   ||.:.....|:.|||.:
Human   276 LTAAQQQQYALAAAQQPHIAGVFSA-GLAPAAFVPNPYIISAAPPGTDPYTAAGLAAAATLAGPA 339

  Fly   776 ----PYYGVAPWGMYPGNLIPQQG---------------TQP-----------RRPLTPSQ---- 806
                .|||| |||:||.||..||.               .||           :|||||:|
Human   340 VVPPQYYGV-PWGVYPANLFQQQAAAAANNTASQQAASQAQPGQQQVLRAGAGQRPLTPNQGQQG 403

  Fly   807 -------------------QG-AENQP-YQVI--PAFLDHTGSLLMG-GPRT--GTPMRLVSPAP 845
                               || |...| |||:  .|:.|.||:|::| |.||  |.|:||::|.|
Human   404 QQAESLAAAAAANPTLAFGQGLATGMPGYQVLAPTAYYDQTGALVVGPGARTGLGAPVRLMAPTP 468

  Fly   846 VLVPPGATRAG---------------------PPPPQGPQLYQPQPQTAQQNLYSQQNGSSVGGL 889
            ||:...|.:|.                     |...|.||..|.||.|   ||   |:.|..|..
Human   469 VLISSAAAQAAAAAAAGGTASSLTGSTNGLFRPIGTQPPQQQQQQPST---NL---QSNSFYGSS 527

  Fly   890 ALNTSSLTGRRDSFDRSTSAFS--PSTMDYTSSGV-------AAAANAVNSTVAQAAAAAAAAAA 945
            :|..||         :|:|.||  |.....||.|.       ||..:|::...:...::|:::|.
Human   528 SLTNSS---------QSSSLFSHGPGQPGSTSLGFGSGNSLGAAIGSALSGFGSSVGSSASSSAT 583

  Fly   946 AR----------GKWPGAMSGAASGAYGALGAGNASASPLGAP---------ITPPP------SA 985
            .|          .:...:::......|.:||. ::|.||:|.|         :||||      |:
Human   584 RRESLSTSSDLYKRSSSSLAPIGQPFYNSLGF-SSSPSPIGMPLPSQTPGHSLTPPPSLSSHGSS 647

  Fly   986 QSCLLG----------SRAPGAESRQRQQQQQQLAAVGLPATAAAAQAAVAAAANNMFGSNSSIF 1040
            .|..||          |.|||||::.|.                      |::.:::|.|:|.:|
Human   648 SSLHLGGLTNGSGRYISAAPGAEAKYRS----------------------ASSTSSLFSSSSQLF 690

  Fly  1041 SNPLAIPGTAAVAAAAAAAAAANSRQVAATAAAAAAVAAAAGGVGGAPQPGRSRLLEDFRNQRYP 1105
                           ..:....|...:..:                    |||||||||||.|:|
Human   691 ---------------PPSRLRYNRSDIMPS--------------------GRSRLLEDFRNNRFP 720

  Fly  1106 NLQLRDLANHIVEFSQDQHGSRFIQQKLERATAAEKQMVFSEILAAAYSLMTDVFGNYVIQKFFE 1170
            |||||||..|||||||||||||||||||||||.||:||||:|||.|||.||||||||||||||||
Human   721 NLQLRDLIGHIVEFSQDQHGSRFIQQKLERATPAERQMVFNEILQAAYQLMTDVFGNYVIQKFFE 785

  Fly  1171 FGTPEQKNTLGMQVKGHVLQLALQMYGCRVIQKALESISPEQQ--QEIVHELDGHVLKCVKDQNG 1233
            ||:.:||..|..:::||||.|||||||||||||||||||.:||  .|:|.|||||||||||||||
Human   786 FGSLDQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQVISEMVKELDGHVLKCVKDQNG 850

  Fly  1234 NHVVQKCIECVDPVALQFIINAFKGQVYSLSTHPYGCRVIQRILEHCTAEQTTPILDELHEHTEQ 1298
            |||||||||||.|.:|||||:||||||:.|||||||||||||||||||||||.|||:|||:||||
Human   851 NHVVQKCIECVQPQSLQFIIDAFKGQVFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQHTEQ 915

  Fly  1299 LIQDQYGNYVIQHVLEHGKQEDKSILINSVRGKVLVLSQHKFASNVVEKCVTHATRGERTGLIDE 1363
            |:||||||||||||||||:.||||.:::.:|||||.||||||||||||||||||:|.||..||||
Human   916 LVQDQYGNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVVEKCVTHASRAERALLIDE 980

  Fly  1364 VCTFND---NALHVMMKDQYANYVVQKMIDVSEPTQLKKLMTKIRPHMAALRKYTYGKHINAKLE 1425
            ||..||   :||:.||||||||||||||||::||.|.|.:|.|||||:..||||||||||.||||
Human   981 VCCQNDGPHSALYTMMKDQYANYVVQKMIDMAEPAQRKIIMHKIRPHITTLRKYTYGKHILAKLE 1045

  Fly  1426 KYYMKITNPITVGTGAGGVP 1445
            |||:|  |...:|. .||.|
Human  1046 KYYLK--NSPDLGP-IGGPP 1062

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
pum	NP_001262403.1	Pumilio	1106..1424	CDD:153420	255/322 (79%)
PUM2	NP_001339846.1	Interaction with SNAPIN. /evidence=ECO:0000269\|PubMed:19168546	1..260		93/373 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	106..204		39/156 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	494..553		23/73 (32%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	620..650		9/29 (31%)
		Pumilio	721..1044	CDD:153420	255/322 (79%)
		Pumilio 1. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00317, ECO:0000269\|PubMed:21397187	726..761		30/34 (88%)
		Adenine-nucleotide binding in RNA target. /evidence=ECO:0000250\|UniProtKB:Q14671	741..745		3/3 (100%)
		Pumilio 2. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00317, ECO:0000269\|PubMed:21397187	762..797		27/34 (79%)
		Uracil-nucleotide binding in RNA target. /evidence=ECO:0000250\|UniProtKB:Q14671	777..781		3/3 (100%)
		Pumilio 3. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00317, ECO:0000269\|PubMed:21397187	798..835		26/36 (72%)
		Adenine-nucleotide binding in RNA target. /evidence=ECO:0000250\|UniProtKB:Q14671	813..817		3/3 (100%)
		Pumilio 4. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00317, ECO:0000269\|PubMed:21397187	836..871		31/34 (91%)
		Non-specific-nucleotide binding in RNA target. /evidence=ECO:0000250\|UniProtKB:Q14671	851..855		3/3 (100%)
		Pumilio 5. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00317, ECO:0000269\|PubMed:21397187	872..907		31/34 (91%)
		Adenine-nucleotide binding in RNA target. /evidence=ECO:0000250\|UniProtKB:Q14671	887..891		3/3 (100%)
		Pumilio 6. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00317, ECO:0000269\|PubMed:21397187	908..943		28/34 (82%)
		Uracil-nucleotide binding in RNA target. /evidence=ECO:0000250\|UniProtKB:Q14671	923..927		3/3 (100%)
		Pumilio 7. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00317, ECO:0000269\|PubMed:21397187	944..979		27/34 (79%)
		Guanine-nucleotide binding in RNA target. /evidence=ECO:0000250\|UniProtKB:Q14671	959..963		3/3 (100%)
		Pumilio 8. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00317, ECO:0000269\|PubMed:21397187	983..1022		24/38 (63%)
		Uracil-nucleotide binding in RNA target. /evidence=ECO:0000250\|UniProtKB:Q14671	1002..1006		3/3 (100%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165148148
Domainoid	1	1.000	146	1.000	Domainoid score	I4546
eggNOG	1	0.900	-	-		E1_COG5099
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	640	1.000	Inparanoid score	I817
Isobase	1	0.950	-	0	Normalized mean entropy	S1682
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000719
OrthoInspector	1	1.000	-	-		otm40372
orthoMCL	1	0.900	-	-		OOG6_100436
Panther	1	1.100	-	-	LDO	PTHR12537
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1032
SonicParanoid	1	1.000	-	-		X849
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	13	12.820