DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment pyd and tjp1b

DIOPT Version :9

Sequence 1:	NP_001246998.1	Gene:	pyd / 41062	FlyBaseID:	FBgn0262614	Length:	2395	Species:	Drosophila melanogaster
Sequence 2:	XP_021326398.1	Gene:	tjp1b / 565459	ZFINID:	ZDB-GENE-070925-1	Length:	2043	Species:	Danio rerio

Alignment Length:	2402	Identity:	624/2402 - (25%)
Similarity:	930/2402 - (38%)	Gaps:	771/2402 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly   189 ERERGGDRDRPYIRNVDDLL---QALGKRDGGDNNSNLLETASTHSSSQRRRFKKGDRTTWEYHT 250
            :...|....:|.:|.:...:   ::|...|..|:||..::                :...||.||
Zfish   218 DESEGSGHGKPSLRRIKGRIHRSKSLDSIDLLDSNSAAMD----------------ETVIWEQHT 266

  Fly   251 VAVTRVPGYGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDRLQVNDRIISVNGVSLENVEY 315
            |.:.|.||:|||||:|||||||||.:|:.||.:||||||||||..||.|||::.||.||::||::
Zfish   267 VTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGLLQENDRVVMVNAVSMDNVDH 331

  Fly   316 ATAVQVLRDSGNTVQLVV--KRRVPLNPINAAGAVQHQHSH---------------------SLS 357
            |.|||.||.||...::.:  ||:|.: |....|..:....|                     |..
Zfish   332 AYAVQQLRKSGKNAKITIRRKRKVQI-PSGRPGERETMSEHDEDDDSYEDEIYESRSARSGPSAY 395

  Fly   358 SVGLMANGSGGVA------------------------PTPITSLSQPNSLNSSLVQNASSGQPIK 398
            |.|....|.||.:                        ...::..|..:|::|:|     ..:|.|
Zfish   396 SGGGGGGGGGGASGRRSGRGDRLSGRRERDRERSGSRERSLSPRSDRHSVSSNL-----PPRPAK 455

  Fly   399 VTLTKGGKKD--------------DYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITRIHN 449
            |||.|..|.:              :||:.|...:|||:||.   |.|.|...::||||::.:|:.
Zfish   456 VTLVKSRKNEACMASGSSQKVTSAEYGLRLASHIFVKDISP---ESLAARDGNIQEGDVVLKING 517

  Fly   450 TNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQTAVSQLNLNNS-----ASHQ---------AS 500
            | ..:.:||.:|||:|:..|.:|.:||.|| ...|.::..:|::|     ||.:         ||
Zfish   518 T-VTENLSLIDAKKLIERSKGKLKMVVQRD-ERATLLNIPDLDDSIPSANASDRDDISDIHSVAS 580

  Fly   501 GNIYATHQPQVSGCSSSNNNLEDPYLPGGASYSSQNLYVQPPTRTSNGPNINGNGLNDEKSNLTP 565
            .:...:|..:.|..|.|.:...:|     :.:|..:     |.:.|||   ...|...:::....
Zfish   581 DHSNRSHDRKRSSRSRSPDRRSEP-----SDHSRHS-----PPQISNG---RATGPTAQRTRPVH 632

  Fly   566 RGRSR------GPIMDGVSLQQLDRPVTPTRGRSAAIDEPPRP-PPPRGSSGGAAQEDFYSSRR- 622
            |.|..      ||:.....:.: :.|...:..|......||.| |.|..:..|....|...|..
Zfish   633 RSRDEDRISKAGPLPLPAKMVE-EMPKEQSAARETEKQLPPLPEPKPVYAQPGQPDVDLPVSPAD 696

  Fly   623 ----QLYEERQSAEP--RFISFQKEGSVGIRLTGGNEAGIFVTAVQPGSPASLQGLMPGDKILKV 681
                .:..:.....|  :.:.|:|..|||:||.|||:.||||..|...|||:.:||..||:||:|
Zfish   697 APVPSVTHDDSILRPSMKLVKFKKGESVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRV 761

  Fly   682 NDMDMNGVTREEAVLFLLSLQ--DRIDLIVQYCKEEYDEVVTNQRGDSFHIKTHFHCDNPSKGEM 744
            |::|...:.|||||||||.|.  :.:.::.|..|:.|..:|.:..||||:|:|||..:..|...:
Zfish   762 NNVDFANIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGL 826

  Fly   745 AFKAGDVFRVIDTLHNGVVGSWQVLKIGRGHQEMQRGVIPNKSRAEELATAQFNATKKEMNANES 809
            :|..|:||||:|||:||.:|||..::||:.|||::||:||||:|||:|::.|:...|   .....
Zfish   827 SFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHQEVERGIIPNKNRAEQLSSVQYTLPK---TPGGD 888

  Fly   810 RGNFFRRRRSTHRRSKSLSRENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARER 874
            |.:|:|.|..  |.||...|::.:|:......:|||||||||||..||:||||:|||::|:|||:
Zfish   889 RADFWRFRGL--RSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVVIFGPIADVAREK 951

  Fly   875 LAKDFPDKF----STPLQDDDKSAATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRLNYAQF 935
            |:::..|.|    |.| :|......:||   |:||..|:.::||.|||:|||||||||||||||:
Zfish   952 LSREETDLFELAKSEP-RDAGTDQRSSG---IIRLHTIKQIIDRDKHAVLDITPNAVDRLNYAQW 1012

  Fly   936 YPVVIFLKTDSKHVIKQLRHGLPKAAHKSSKKLLEQCQKLERVWSHIFSTQIALSD-EESWYRKL 999
            ||:|:||..|||..:|.:|..|...:.||::||.|:..||.:...|:|:|.|.|:: .:.||..|
Zfish  1013 YPIVVFLNPDSKQGVKNMRTRLCPESRKSARKLYERALKLRKNNHHLFTTTINLNNMNDGWYGAL 1077

  Fly  1000 RDSIDLQQSGAVWMSESKPVESLSDDFLFPMTTSRLSYASSPES----------------DLELS 1048
            ::||..||:..||:||.|...:..||  ..:...||||.|:|.|                |.:..
Zfish  1078 KESIQQQQNQLVWVSEGKADGAPDDD--LDIHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTE 1140

  Fly  1049 PGPSASLSLG-NLPQLVKASSDPSIATNQDNLDRDRDIIGEGLPPPYTVPYDHAVPANPNRRQTM 1112
            .|......|. .:...|...|:|:|..:.:.:..|..:|.:....|   .|.|||| .|.....:
Zfish  1141 GGAYTDQELDETMNDEVGLPSEPAITRSSEPVREDPPVIQDAAGYP---SYQHAVP-QPEAVSRI 1201

  Fly  1113 DSSKYSIYGTNVPPQQQQPGVGG---DTAAVRP-QSLYGINAPDLPPRIDRQSKPGDIPLNTSGS 1173
            |.:.:.:   ..|.||.:..:..   .:|||:| .:|.|.| |:.||
Zfish  1202 DPAGFKM---AAPQQQAEAALASPPPPSAAVQPTATLAGTN-PEEPP------------------ 1244

  Fly  1174 SSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSGAAETLDRQQQQQQQTHASLERQARLN 1238
                                                |:..|::|
Zfish  1245 ------------------------------------GTPQADSL--------------------- 1252

  Fly  1239 AQLKANGVGAGGGGASTYDSVSSYDSYNNTQMAMQNLGRLGPNAPDDLKSVPNANGRPLPPTGQ- 1302
                                                      |:|     ||..:.:|.|...|
Zfish  1253 ------------------------------------------NSP-----VPVPDPQPEPELAQP 1270

  Fly  1303 -SHEYGRTPHDHRSFGGPNDLNRQSSPGRPHYHDMNASRNIDPRNGTPQRPSNLGLESSPRKPLV 1366
             :|:    ||             ||.|..|           ||:              ..:|.|
Zfish  1271 PTHD----PH-------------QSLPPGP-----------DPK--------------MYKKDL- 1292

  Fly  1367 ETKTDYGKYSRNNSVTQADYTKLPKTAPHGVVPPPNVSNGQGQMNGSGTPSS---------NGSG 1422
                    ||.:.          |....|||.|||    .|.|....|.|:|         ....
Zfish  1293 --------YSMDE----------PVRVTHGVKPPP----PQQQQQPLGPPTSLSYSHQPVYQDQQ 1335

  Fly  1423 PFKPV--PP----------PKPKNYRPPVQSGGSSGSGGTTPWENGDSGSPRSPNGFYYPPTPSH 1475
            |::..  ||          |||.||.|.:.            ::|      |.|:  |....|::
Zfish  1336 PYRQYDHPPYGYDGGGYAQPKPHNYDPHLH------------YDN------RVPH--YNEQWPTY 1380

  Fly  1476 HHYGQQATPGSPSNGHMQPPP----PQQQQPTYGGSNGNYGQAPPPQSYPQANGYNGNGHHYNGG 1536
            ....||.|  ||     ||||    ||..||          ..|||..|...:.|..
Zfish  1381 DQQQQQQT--SP-----QPPPASGFPQGHQP----------PPPPPMGYEPRSPYED-------- 1420

  Fly  1537 SGTGPYIAPHRGMPPPIGNLPPHTPERHALDLAGSREQRGSAFELYRKPQIGATAGHHHNMSEME 1601
                   .|:|...||.......:|                             .|:.|.....:
Zfish  1421 -------GPNRDFSPPQSQYDGVSP-----------------------------MGYDHRPRHPK 1449

  Fly  1602 PYDERYDDYYNMPPPAAHPSQGHHMQRSRSAPRYPHERPPHAQDPNYYGHYGTSRGHSQPRQQYS 1666
            |...||::....|||.::.:            |.|.|..|||...|      |.|....|:|.|.
Zfish  1450 PAPVRYEEPPPPPPPVSYDA------------RSPFEPEPHAFPGN------THRSPDPPKQYYG 1496

  Fly  1667 QHHQQQYYDDHGLEMGPPPLPPHKKKKSVLKSPLVALKNALLKSTRPLRRMNSMVEPERKPKGLR 1731
            ....:..|:         |.||::           ..|:..:.|..|:        |..||
Zfish  1497 DAAMRPLYN---------PGPPNR-----------VYKHEPVNSEPPV--------PPPKP---- 1529

  Fly  1732 RQQSMLERGVQRPYYPDEYPTYPAGFEERAHGGQGMMQPHSQDAYYQRGGHYAPQQQEMMNSTYQ 1796
              :|:|..| :.|:.....|..|              .|...|.........|.:.|.::|
Zfish  1530 --ESVLSPG-EPPHSAASKPLPP--------------PPRDDDDDDDDDDDPAMKPQSVLN---- 1573

  Fly  1797 NLEGEDIYGNIGNTVPRMPHHQDNGYGYDQYDLYANRACIDLERRQAEAAAASGGRNGRRIVRRH 1861
                                         :..::.|:..:.::|.: :|...:|.|
Zfish  1574 -----------------------------RVKMFENKRSVSVDRAK-DAPDVTGIR--------- 1599

  Fly  1862 STTTADRGGNPGP---------------------PRRPMISPG--------YEQDPQEIYQTRNG 1897
            .|........|||                     |::|....|        |:.|..|.|..:..
Zfish  1600 PTDLPKPASTPGPVLKANSLSNLEQDKPSYRAPEPQKPQTRVGDDVVRSNHYDPDDDEEYYRKQL 1664

  Fly  1898 AYMLPDQRRAPSSEVMTRRRFYPGAANEQTEEEPLYQSRREMQREMQRNHLYQ-SKREMQERISQ 1961
            ::.   .||:..|:||..    |.|...:..|.|         :..|....|. ::.|..|::|
Zfish  1665 SFF---DRRSFDSKVMNP----PSAGVNRFHEPP---------KPPQTQIAYPFARTESVEKVS- 1712

  Fly  1962 GKRDMEREFSPQSSSSQSEASNSEAIYQSRREAKESALKTRSQLRDQIYQTRREALDSMAEPIYV 2026
               .:::.:.|....:.|.|..|:.                               ..:|.|..:
Zfish  1713 ---PVDKRYEPLPPVNPSPAPYSQN-------------------------------TPVAPPTSL 1743

  Fly  2027 SKRGMGRPAPIYETREESILQSRENETDEKKEKECKTEQIQVEINAQPEEQIEDSTLSRSDLQKS 2091
            .|                                    ...:|:|:.|:.  ..|..::.||
Zfish  1744 PK------------------------------------LTNIEVNSLPDP--HSSPKAKPDL--- 1767

  Fly  2092 SDTVIENPARAPLVQDEVDDDEEEEVQDQRDPAESSADQTEQP-----TAIENNLHNEGMVEQSQ 2151
                  :..|||        ..:|.:|....|.:|:.:..:.|     .....|.:.......|.
Zfish  1768 ------SALRAP--------TRDEPIQSSYLPPKSTINGMDAPPKTLGVPTSYNRYVPKPYTSSA 1818

  Fly  2152 NDSDDVFEAADKVSPLAPPPAPVQPPTPRSGRAPFHISNILK-RTAPPPSSPIGDSCTSIETQYT 2215
            ...:..||:......|.|.....:|          .::|.|| :.:|.|.    |:.:.::|...
Zfish  1819 RPFERKFESPKFNHNLLPNDTQSKP----------SVNNNLKPQISPQPQ----DTDSGVDTFTR 1869

  Fly  2216 SQASLPVGPPNATSTPFSSSMSLPIAGPVNNQPSA-------NGRFPTLPREPSTSRGFFDSNGG 2273
            :..:.|....|..:       ::|.|.||:  |||       .|....     :|:||.|:.|||
Zfish  1870 TMDNRPKYQHNNIN-------AIPKAVPVS--PSALDDDEEDEGHTVV-----ATARGIFNCNGG 1920

  Fly  2274 TLADKLWHVSLQIPPGAIPAGVRQEIYFTVSDPRMGQAVGGPPLDMENGETMLSPLVMCGPQGLE 2338
            .|:.....||:.||.||||..|.|||||.|.  |....:  ||||.|.|||:|||||||||.||:
Zfish  1921 VLSSIETGVSIIIPQGAIPDSVEQEIYFKVC--RDNSIL--PPLDKEKGETLLSPLVMCGPHGLK 1981

  Fly  2339 FLVPVTLNIPHCAGRTA-SLGLALKATDSEKNLHTEWDNIDLPSN----AAAHTVSVKVDHF 2395
            ||.||.|.:||||..|. ....|||::||.......|.|..||.:    ..|:.|||.:|||
Zfish  1982 FLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKCWQNKCLPGDPNYLVGANCVSVLIDHF 2043

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
pyd	NP_001246998.1	PDZ_signaling	249..334	CDD:238492	52/86 (60%)
		PDZ_signaling	396..477	CDD:238492	33/94 (35%)
		PDZ_signaling	633..710	CDD:238492	38/80 (48%)
		SH3_ZO	729..790	CDD:212793	31/60 (52%)
		GuKc	831..1007	CDD:214504	85/180 (47%)
		ZU5	2263..2351	CDD:279170	50/87 (57%)
tjp1b	XP_021326398.1	CARD	59..132	CDD:260018
		PDZ_signaling	265..350	CDD:238492	52/84 (62%)
		PDZ	477..546	CDD:214570	27/72 (38%)
		Atrophin-1	<606..703	CDD:331285	20/105 (19%)
		PDZ	720..792	CDD:278991	36/71 (51%)
		SH3_ZO-1	808..872	CDD:212959	34/63 (54%)
		GuKc	906..1085	CDD:214504	86/182 (47%)
		Atrophin-1	<1171..1906	CDD:331285	211/1249 (17%)
		ZU5	1910..2009	CDD:307095	56/102 (55%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170590009
Domainoid	1	1.000	113	1.000	Domainoid score	I6090
eggNOG	1	0.900	-	-		E1_KOG3580
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	614	1.000	Inparanoid score	I868
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1175136at2759
OrthoFinder	1	1.000	-	-		FOG0001438
OrthoInspector	1	1.000	-	-		otm25454
orthoMCL	1	0.900	-	-		OOG6_103390
Panther	1	1.100	-	-	O	PTHR13865
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X1692
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	13	12.850