DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment pyd and Scrib

DIOPT Version :9

Sequence 1:	NP_001246998.1	Gene:	pyd / 41062	FlyBaseID:	FBgn0262614	Length:	2395	Species:	Drosophila melanogaster
Sequence 2:	NP_001178808.1	Gene:	Scrib / 362938	RGDID:	1565055	Length:	1663	Species:	Rattus norvegicus

Alignment Length:	1508	Identity:	280/1508 - (18%)
Similarity:	473/1508 - (31%)	Gaps:	476/1508 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly    49 YQIADRVYTTSKMFLRDGYMYPRQ-------PGQTMDP---------------RF--PLELHEFP 89
            :|..|...|..|:.  ..|:.|:|       |||...|               :|  |||..|..
  Rat   398 FQTEDDAQTGEKVL--TCYLLPQQPLPSLEDPGQQSSPSESCSDAPLSRVSVIQFEDPLEGEEDA 460

  Fly    90 QQFGA--TGLQLVSAP-----KVHIAG------EYLASEQQQQQQQQQQQHQQVQEQQQLGRRKA 141
            ::..|  .|||..:.|     ||...|      |....:.........::.:::..:..|.....
  Rat   461 EEAAAEKRGLQRRATPHPSELKVMKRGIEERRNEAFVCKPDPSPPSPSEEERRLSAESALSGNSV 525

  Fly   142 AAAAEIN------LANQVYWLGKQKTRS----------RSRSNSVGNRSITSCMGGGGGGGEKE- 189
            .:|:..:      |..:|..||:|:...          ...:......::.....|.|..|:.|
  Rat   526 PSASTASDGEPETLPAEVQGLGQQEAMPGQEEYPEEDYNEPTVHFAEDTLIPREDGEGEEGQPEA 590

  Fly   190 -------RERGGDRDRPYIRN------------VDDLLQAL-----GKRDGGDNNSNLLETASTH 230
                   |:|...:|.|:.:.            |..|||.:     |...|..|..:.......|
  Rat   591 PWPLPSGRQRLIRKDTPHYKKHFKISKLPQPEAVVALLQGVQTDREGPTAGWHNGPHTPWAPRAH 655

  Fly   231 ---SSSQRRRFKKGD----------------------------------RTTWEYHTVAVTRVPG 258
               ...:.|..::|:                                  |...|..|:.:.|..|
  Rat   656 EEEEEEENRDEEEGEASMEEDDKEEAVASAPSVKGVSFDQANNLLIEPARIEEEELTLTIVRQTG 720

  Fly   259 YGFGIAVSGGRDNPHFANGDPSIAVSDVLKGGPAEDR-LQVNDRIISVNGVSLENVEYATAVQVL 322
             |.||:::||:.:..:...|..|.:|.|.:.|||... ::|.|:::.||||:|::.|:..||:.|
  Rat   721 -GLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAAHAGVRVGDKLLEVNGVALQDAEHHEAVEAL 784

  Fly   323 RDSGNTVQLVVKRRVPLNPINAAGAVQHQHSHSLSSVGLMANGSGGVAPTPITSLSQPNSL---N 384
            |.:|..||:.|.|...:.|.||......:.....|.    ....||....|:.....|..|   :
  Rat   785 RGAGAAVQMRVWRERMVEPENAVTITPLRPEDDYSP----REWRGGGLRLPLLQPETPVPLRQRH 845

  Fly   385 SSLVQNASSGQPIKVTLTKGG---KKDDYGVVLGCRLFVKEISSKAREQLNANGYSLQEGDIITR 446
            ::.:..:..|....:...||.   :..|.|:      |:..|:......   ...:||.||.:..
  Rat   846 AACLVRSEKGLGFSIAGGKGSTPYRAGDAGI------FISRIAEGGAAH---RAGTLQVGDRVLS 901

  Fly   447 IHNTNCGDTMSLKEAKKIIDGCKERLNLVVLRDITNQTAVSQLNLNNSASHQAS---GNIYATHQ 508
            |:..:..:... ..|..::......::|::.|:.......|      ...|.:|   ..:.||..
  Rat   902 INGVDMTEARH-DHAVSLLTAASTTISLLLERETGGAYPPS------PPPHSSSTPTATVAATMT 959

  Fly   509 PQVSG--------CSSSNNNLEDPY------LP-----------GGASYSSQNLYVQPPTRTSNG 548
            ..:.|        .|.....||.||      ||           ||:.:||....||.|     |
  Rat   960 TAIPGEPLLPRLSPSLLATALEGPYPVEEICLPRAGGPLGLSIVGGSDHSSHPFGVQDP-----G 1019

  Fly   549 PNINGNGLNDEKSNLTPRGRSR------GPIMDGVSLQQLDRPVTPTRGRSAAIDEP-------- 599
            ..|         |.:.|||.:.      |..:..|:.|.: |..|.....||.: .|
  Rat  1020 VFI---------SKVLPRGLAARCGLRVGDRILAVNGQDV-REATHQEAVSALL-RPCLELCLLV 1073

  Fly   600 PRPPPPRGSSGGAAQEDFYSSRRQLYEERQSAEPRFISFQKEGSVGIRLTGG-----------NE 653
            .|.|||.|             .|:|..::...|          .:||.:.||           .:
  Rat  1074 RRDPPPPG-------------MRELCIQKAPGE----------KLGISIRGGAKGHAGNPCDPTD 1115

  Fly   654 AGIFVTAVQPGSPASLQG-LMPGDKILKVNDMDMNGVTREEAVLFLLSLQDRIDLIVQYCKEEYD 717
            .|||::.|.|...|...| |..|.::|:||...:.|:|..|||..|.|:.|.:.::|
  Rat  1116 EGIFISKVSPTGAAGRDGRLRVGLRLLEVNQQSLLGLTHAEAVQLLRSVGDTLTVLV-------- 1172

  Fly   718 EVVTNQRGDSFHIKTHFHCDNPSKGEMAFKAGDVFRVIDT-------LHNGVVGSWQVLKIGRGH 775
                              ||.                .||       :..||:.:  ....|.||
  Rat  1173 ------------------CDG----------------FDTSTTTALEVSPGVIAN--PFAAGLGH 1201

  Fly   776 Q-------EMQRGVIP-NKSRAEELATAQFNATKKEMNANESRGNFFRRRRSTHRRSKSL----S 828
            :       .:.|.:.| ...:.:|||:   .....|..:.|:.|......:..:|...::    |
  Rat  1202 RNSLESISSIDRELSPEGPGKEKELAS---QTLPWEPESAETTGRNQEPLKLDYRVLAAVPSAGS 1263

  Fly   829 RENWDDVVFSDSISKFPAYERVVLRHPGFVRPVVLFGPVSDLARERLAKDFPDKFSTPLQDDDKS 893
            .:..........:::.|:........||.::|              ||:.:|       :|....
  Rat  1264 LQRGPCATTGGKMTEAPSSPGSQQTKPGVIQP--------------LAQAWP-------RDSPAP 1307

  Fly   894 AATSGKCRIVRLSNIRDVMDRGKHALLDITPNAVDRL--NYAQFYPVVIFLKTDSKHVIKQLRHG 956
            ....|.|                      :|.:.|.|  |..|.|..  |....:.|..:.....
  Rat  1308 RGRDGPC----------------------SPPSPDELPANVKQAYRA--FAAVPTVHPPENSATQ 1348

  Fly   957 LPKAAHKSSKKLLEQCQKLERVWSHIFSTQIALSDEESWYRKL----RDSIDLQQSGAVWMSESK 1017
            .|.....:|.   ||....||  ...|..::.:...|...:::    .|.:...|.......:.|
  Rat  1349 PPTPGPAASP---EQLSFRER--QKYFELEVRVPQAEGPPKRVSLVGADDLRKMQEEEARKLQQK 1408

  Fly  1018 PVESLSDDFLFPMTTSRLSYASSPESDLELSPGPSASLSLGNLPQLVKASSDPSIATNQDNLDRD 1082
            ..:.|.::                    .::.||                 |..:|:::::||.:
  Rat  1409 RAQMLREE--------------------AVTSGP-----------------DTGLASDRESLDDE 1436

  Fly  1083 RDIIGEGLPPPYTVPYDHAVPANPNRRQTMDSSKYSIYGTNVPPQQQQPGVGGDTAAVRPQSLYG 1147
            ::     ...|:..| .||...:|        |..:..|.:.|.:..:       |..|.|....
  Rat  1437 QE-----AEQPWAGP-SHAGGLSP--------SSPTPLGGSAPVRTAK-------AERRHQERLR 1480

  Fly  1148 INAPDLPPRIDRQSKPGDIPLNTSGSSSRNGTLGRSAQERLFGKAVVQDDVQAEYITRNALVGSG 1212
            :.:|:||.. :|...|.:    .....:....|.|:|:.    |::.||.::|:.:...:..|.|
  Rat  1481 MQSPELPAP-ERALSPAE----RRALEAEKRALWRAARM----KSLEQDALRAQMVLSKSQEGRG 1536

  Fly  1213 AAETLDRQQQQQQ---------------QTHASLERQARLNAQLKANGVGAGGGGASTYDSVSSY 1262
            ....|:|..:...               ||..|..|......:.......|.......||..|
  Rat  1537 KRGPLERLAEAPSPAPTPSPTPLEDFGLQTSTSPGRLPLSGKKFDYRAFAALPSSRPVYDIQS-- 1599

  Fly  1263 DSYNNTQMAMQNLGRLGPNAPDDLKSVPNANGRPLPPTGQSHEYGRTP-----HDHRSFGGPNDL 1322
                             |:..::|:::.     |.|..|...|.|...     .......||.|:
  Rat  1600 -----------------PDFVEELRTLD-----PSPSPGPQEEDGEVALVLLGRPSPGTVGPEDM 1642

  Fly  1323 NRQSS-----PGR 1330
            ...||     |||
  Rat  1643 TLCSSRRAMRPGR 1655

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
pyd	NP_001246998.1	PDZ_signaling	249..334	CDD:238492	30/85 (35%)
		PDZ_signaling	396..477	CDD:238492	13/83 (16%)
		PDZ_signaling	633..710	CDD:238492	24/88 (27%)
		SH3_ZO	729..790	CDD:212793	11/75 (15%)
		GuKc	831..1007	CDD:214504	27/181 (15%)
		ZU5	2263..2351	CDD:279170
Scrib	NP_001178808.1	LRR_RI	12..232	CDD:238064
		leucine-rich repeat	14..37	CDD:275380
		leucine-rich repeat	38..60	CDD:275380
		LRR	39..405	CDD:227223	2/6 (33%)
		leucine-rich repeat	61..83	CDD:275380
		leucine-rich repeat	84..106	CDD:275380
		leucine-rich repeat	107..129	CDD:275380
		leucine-rich repeat	130..152	CDD:275380
		leucine-rich repeat	153..175	CDD:275380
		leucine-rich repeat	176..198	CDD:275380
		leucine-rich repeat	199..221	CDD:275380
		leucine-rich repeat	268..290	CDD:275380
		LRR_4	289..329	CDD:289563
		leucine-rich repeat	291..313	CDD:275380
		leucine-rich repeat	314..336	CDD:275380
		leucine-rich repeat	360..381	CDD:275380
		PDZ	709..797	CDD:214570	32/88 (36%)
		PDZ_signaling	852..931	CDD:238492	14/88 (16%)
		PDZ_signaling	986..1074	CDD:238492	23/103 (22%)
		PDZ_signaling	1083..1173	CDD:238492	28/125 (22%)
		AbLIM_anchor	1563..>1657	CDD:292800	23/117 (20%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	2	1.910