DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment hb and REST

DIOPT Version :9

Sequence 1:	NP_731267.1	Gene:	hb / 41032	FlyBaseID:	FBgn0001180	Length:	758	Species:	Drosophila melanogaster
Sequence 2:	NP_001180437.1	Gene:	REST / 5978	HGNCID:	9966	Length:	1097	Species:	Homo sapiens

Alignment Length:	991	Identity:	189/991 - (19%)
Similarity:	279/991 - (28%)	Gaps:	434/991 - (43%)

- Green bases have known domain annotations that are detailed below.

  Fly   134 GLPNPMQHFYGGNLRPSPQPTPTSASTIAPVAVATGSSEKLQALTPPMDVTPPKSPAKSSQSNIE 198
            |.|:    .|..|....|:         .|.|...|.|.|    |.|....|.:..|:|.:..:.
Human   129 GAPD----IYSSNKDLPPE---------TPGAEDKGKSSK----TKPFRCKPCQYEAESEEQFVH 176

  Fly   199 PEKEHDQ----MSNSSEDMKYMAES-----EDDD--------------TNIRMPIYNSHGK---- 236
            ..:.|..    :..|:|......||     |:.|              || |...|.:|.|
Human   177 HIRVHSAKKFFVEESAEKQAKARESGSSTAEEGDFSKGPIRCDRCGYNTN-RYDHYTAHLKHHTR 240

  Fly   237 ----MKNYKCKTCGVVAITKVDFWAHTRTHMKPDKILQCPK------------------------ 273
                .:.|||..|....:::..:..|.|.|. |.|:..|.|
Human   241 AGDNERVYKCIICTYTTVSEYHWRKHLRNHF-PRKVYTCGKCNYFSDRKNNYVQHVRTHTGERPY 304

  Fly   274 ----CPFVTEFKHHLEYHIRKHKNQKPFQCDKCSYTCVNKSMLNSH-RKSHSSVYQYRCADCDYA 333
                ||:.:..|.||..|:|.|..:|||:||:|||...|:..:..| |:.|:......|..|||.
Human   305 KCELCPYSSSQKTHLTRHMRTHSGEKPFKCDQCSYVASNQHEVTRHARQVHNGPKPLNCPHCDYK 369

  Fly   334 TKYCHSFKLHLR-----------------------KYGHK------PGMVLD------------- 356
            |....:||.|:.                       :|..|      |...:|
Human   370 TADRSNFKKHVELHVNPRQFNCPVCDYAASKKCNLQYHFKSKHPTCPNKTMDVSKVKLKKTKKRE 434

  Fly   357 ---EDGTPNPSLVI-------DVYG--------------------------------TRR----- 374
               .|...|....|       ||.|                                ||:
Human   435 ADLPDNITNEKTEIEQTKIKGDVAGKKNEKSVKAEKRDVSKEKKPSNNVSVIQVTTRTRKSVTEV 499

  Fly   375 --------------------------------GP----------------KSKNGG-PIASGGS- 389
                                            ||                ||||.. .:..|.|
Human   500 KEMDVHTGSNSEKFSKTKKSKRKLEVDSHSLHGPVNDEESSTKKKKKVESKSKNNSQEVPKGDSK 564

  Fly   390 ---------------------GSGSRKSN-----------VAAVAPQQQ-------------QSQ 409
                                 ...|:||:           .|.:.|.|.             |.:
Human   565 VEENKKQNTCMKKSTKKKTLKNKSSKKSSKPPQKEPVEKGSAQMDPPQMGPAPTEAVQKGPVQVE 629

  Fly   410 PAQPVATSQLSAALQGFPLVQGNSAP------------PAASPVLPLPASPAKSVASVEQTPSLP 462
            |..|:..:|:..|       |...||            ||...:|| |..||:.|.:......:.
Human   630 PPPPMEHAQMEGA-------QIRPAPDEPVQMEVVQEGPAQKELLP-PVEPAQMVGAQIVLAHME 686

  Fly   463 SPANLLPPLASLLQQNRNMAFFPYWNLNLQMLAAQQQAA---------VLAQLSPRMREQLQQQN 518
            .|    ||:.:...:...|...|.  ...||..||.::|         |..:|||.| |.:|::.
Human   687 LP----PPMETAQTEVAQMGPAPM--EPAQMEVAQVESAPMQVVQKEPVQMELSPPM-EVVQKEP 744

  Fly   519 QQ----------QSDNEEEEQDDEYE---RKSVDSAMDLSQGTPVKEDEQQQQP---QQPLAMN- 566
            .|          |.:..:.|.....|   ::.|...:....|...||..|::.|   :.||.|.
Human   745 VQIELSPPMEVVQKEPVKIELSPPIEVVQKEPVQMELSPPMGVVQKEPAQREPPPPREPPLHMEP 809

  Fly   567 ------LKVEEEATPLMSSSNASRRKGRVLKLDTLLQLRS----EAMTSPEQLKVPSTPMPTAS- 620
                  |:.:::....|.|..|  ||.:||....|:.::.    :...|.|.|..||.|:|..:
Human   810 ISKKPPLRKDKKEKSNMQSERA--RKEQVLIEVGLVPVKDSWLLKESVSTEDLSPPSPPLPKENL 872

  Fly   621 -----------SPIAGRKPMPEEHCSGTSSADESM--------ETAHV---------PQANT--- 654
                       :...|.|..|.:.. |...||||:        |:.|:         |:..|
Human   873 REEASGDQKLLNTGEGNKEAPLQKV-GAEEADESLPGLAANINESTHISSSGQNLNTPEGETLNG 936

  Fly   655 -----------------------------------------------SASSTASSS--------- 663
                                                           :.|.||.:|
Human   937 KHQTDSIVCEMKMDTDQNTRENLTGINSTVEEPVSPMLPPSAVEEREAVSKTALASPPATMAANE 1001

  Fly   664 --------GNSSNASSNSNGNSSSNSSSNGTTSAVAAPPSGT---------PAAAGAIYECKYCD 711
                    |..|:..|:.:.|.|..|..:|...|...|...:         ..||...:.|.:||
Human  1002 SQEIDEDEGIHSHEGSDLSDNMSEGSDDSGLHGARPVPQESSRKNAKEALAVKAAKGDFVCIFCD 1066

  Fly   712 IFFKDAVLYTIHMGYH 727
            ..|:....|:.|:..|
Human  1067 RSFRKGKDYSKHLNRH 1082

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
hb	NP_731267.1	C2H2 Zn finger	242..262	CDD:275368	4/19 (21%)
		C2H2 Zn finger	271..291	CDD:275368	9/47 (19%)
		zf-H2C2_2	283..308	CDD:290200	13/24 (54%)
		C2H2 Zn finger	299..319	CDD:275368	8/20 (40%)
		C2H2 Zn finger	327..345	CDD:275368	8/17 (47%)
		C2H2 Zn finger	707..727	CDD:275371	6/19 (32%)
		C2H2 Zn finger	735..757	CDD:275371
REST	NP_001180437.1	Interaction with SIN3A. /evidence=ECO:0000269\|PubMed:10734093	32..122
		Interaction with SIN3B. /evidence=ECO:0000269\|PubMed:16288918	43..57
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	83..103
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	127..159		11/46 (24%)
		Interaction with ZFP90. /evidence=ECO:0000269\|PubMed:21284946	145..418		65/278 (23%)
		Required for binding to the neuron-restrictive silencer element. /evidence=ECO:0000250\|UniProtKB:Q8VIG1	201..212		3/10 (30%)
		C2H2 Zn finger	250..270	CDD:275368	4/19 (21%)
		C2H2 Zn finger	278..298	CDD:275368	2/19 (11%)
		COG5048	302..>381	CDD:227381	28/78 (36%)
		C2H2 Zn finger	306..326	CDD:275368	7/19 (37%)
		zf-H2C2_2	318..343	CDD:316026	13/24 (54%)
		C2H2 Zn finger	334..355	CDD:275368	8/20 (40%)
		C2H2 Zn finger	363..383	CDD:275368	8/19 (42%)
		C2H2 Zn finger	391..407	CDD:275368	0/15 (0%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	452..642		23/189 (12%)
		FtsK	<599..>767	CDD:332908	39/182 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	774..837		15/64 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	853..938		19/85 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	961..1049		13/87 (15%)
		Interaction with RCOR1. /evidence=ECO:0000269\|PubMed:10449787	1009..1087		18/74 (24%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165156900
Domainoid	1	1.000	76	1.000	Domainoid score	I9000
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D264318at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	3	2.940