DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment hb and PLAG1

DIOPT Version :9

Sequence 1:	NP_731267.1	Gene:	hb / 41032	FlyBaseID:	FBgn0001180	Length:	758	Species:	Drosophila melanogaster
Sequence 2:	NP_001108106.1	Gene:	PLAG1 / 5324	HGNCID:	9045	Length:	500	Species:	Homo sapiens

Alignment Length:	585	Identity:	108/585 - (18%)
Similarity:	177/585 - (30%)	Gaps:	199/585 - (34%)

- Green bases have known domain annotations that are detailed below.

  Fly   187 KSPA-KSSQSNIEPEKEH-----DQMSNSSEDMKYMAESEDDDTNIRMPIYNSHGKMKNYKC--K 243
            |.|: |..:...:|.|..     |:..||.|.:|..:              .||...:.|||  :
Human    17 KVPSGKRKRGETKPRKNFPCQLCDKAFNSVEKLKVHS--------------YSHTGERPYKCIQQ 67

  Fly   244 TCGVVAITKVDFWAHTRTHMKPDKILQCPKCPFVTEFKHHLEYHIRKH-KNQKPFQCDKC----- 302
            .|....::|.....|..|| .|:|..:|..|..:...|.||:.|:..| .|::.|:|::|
Human    68 DCTKAFVSKYKLQRHMATH-SPEKTHKCNYCEKMFHRKDHLKNHLHTHDPNKETFKCEECGKNYN 131

  Fly   303 -----------------SYTC-------VNKSMLNSHRKSHS-------SVYQYRCADC------ 330
                             ..||       .:..:|..|.|||:       ...:::|..|
Human   132 TKLGFKRHLALHAATSGDLTCKVCLQTFESTGVLLEHLKSHAGKSSGGVKEKKHQCEHCDRRFYT 196

  Fly   331 ---------------DYATKYCHSFKLHLRKYGHKPGMVLDEDGTPNPSLVIDVYGTRRGPKSKN 380
                           |:..:||      .:::|.|..:                  ||...||.|
Human   197 RKDVRRHMVVHTGRKDFLCQYC------AQRFGRKDHL------------------TRHMKKSHN 237

  Fly   381 GGPIASGGSGSGSRKSNVAAVAPQQQQSQPAQPVATSQLSAALQGFPLVQGNSAPPAASPVLPLP 445
                                   |:......:||      ..|..|..   |.:.|....:||:
Human   238 -----------------------QELLKVKTEPV------DFLDPFTC---NVSVPIKDELLPV- 269

  Fly   446 ASPAKSVASVEQTPSLPSPANLLPPLASLLQQNRNMAFFPYWNLNLQMLAAQQQA-AVLAQLSPR 509
                         .||||...|..|..:.||.|       .:|...|.:.:...| .::..|...
Human   270 -------------MSLPSSELLSKPFTNTLQLN-------LYNTPFQSMQSSGSAHQMITTLPLG 314

  Fly   510 MREQLQQQNQQQSDN----------------EEEEQDDEYERKSVDSAMDLSQGTPVKEDEQQQQ 558
            |...:.......|.:                .|:||..:.|.:|.  .|:|..|.|....:.|..
Human   315 MTCPIDMDTVHPSHHLSFKYPFSSTSYAISIPEKEQPLKGEIESY--LMELQGGVPSSSQDSQAS 377

  Fly   559 PQQPLAMNLKVEEEATPLMSSSNASRRKGRVLKLDTLLQLRSEAMTSPEQLKVPSTPMPTASSPI 623
            ....|.::        |.:.|.:.....         |.|...:::..:.|..|:.......:.|
Human   378 SSSKLGLD--------PQIGSLDDGAGD---------LSLSKSSISISDPLNTPALDFSQLFNFI 425

  Fly   624 AGRKPMPEEHCSGTSSADESMETAH-----VPQANTSASSTASSSGNSSNASSNSNGNSSSNSSS 683
            ....|.......|:.....|.|.||     :|.........|::.|..|..|.::...||.::|:
Human   426 PLNGPPYNPLSVGSLGMSYSQEEAHSSVSQLPPQTQDLQDPANTIGLGSLHSLSAAFTSSLSTST 490

Fly 684 683
Human 491 490

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
hb	NP_731267.1	C2H2 Zn finger	242..262	CDD:275368	4/21 (19%)
		C2H2 Zn finger	271..291	CDD:275368	6/19 (32%)
		zf-H2C2_2	283..308	CDD:290200	10/54 (19%)
		C2H2 Zn finger	299..319	CDD:275368	7/48 (15%)
		C2H2 Zn finger	327..345	CDD:275368	5/38 (13%)
		C2H2 Zn finger	707..727	CDD:275371
		C2H2 Zn finger	735..757	CDD:275371
PLAG1	NP_001108106.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..30		3/12 (25%)
		Interaction with KPNA2. /evidence=ECO:0000269\|PubMed:11882654	2..84		18/80 (23%)
		Nuclear localization signal	22..25		1/2 (50%)
		C2H2 Zn finger	36..56	CDD:275368	5/33 (15%)
		Decreased nuclear import with localization in the nucleus but also in the cytoplasm	41..242		47/262 (18%)
		SFP1	<58..139	CDD:227516	21/81 (26%)
		C2H2 Zn finger	64..86	CDD:275368	4/21 (19%)
		zf-H2C2_2	79..103	CDD:404364	7/24 (29%)
		C2H2 Zn finger	94..114	CDD:275368	6/19 (32%)
		C2H2 Zn finger	123..143	CDD:275368	2/19 (11%)
		C2H2 Zn finger	152..172	CDD:275368	4/19 (21%)
		C2H2 Zn finger	187..207	CDD:275368	2/19 (11%)
		C2H2 Zn finger	215..233	CDD:275368	6/41 (15%)
		Activates transcription, Inhibition of nuclear import due to lack of NLS and KPNA2 interaction	243..500		55/297 (19%)
		Repression domain, contains 3 sumoylation motifs and massively decrease transcription activity	243..384		34/172 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	365..388		5/30 (17%)
		Massively activates transcription	385..500		21/123 (17%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165156892
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.930