Protein Alignment 5-HT2B and mAChR-B

DIOPT Version :10

• Sequence 1: NP_001262373.1
• Gene: 5-HT2B / 41017
• FlyBaseID: FBgn0261929
• Length: 947
• Species: Drosophila melanogaster
• Sequence 2: NP_649764.1
• Gene: mAChR-B / 40955
• FlyBaseID: FBgn0037546
• Length: 1019
• Species: Drosophila melanogaster
• Alignment Length: 1072
• Identity: 220/1072 - (20%)
• Similarity: 377/1072 - (35%)
• Gaps: 320/1072 - (29%)

  Fly    12 YNDSGG-DDWSSSEHLVLWEEDETQRTTANATSRHNQLHVARWNATGNATISATFED-------- 67
            |.:|.. :.|..:|.|..:...:...|..       .|.|...::.|.:|:|.:..|
  Fly    68 YGESSDFNSWPVNEQLQWYRNFDVNNTNL-------LLAVRNQSSDGGSTMSGSSSDSEILGPVL 125

  Fly    68 VPFDANNYW-----ALLALVLVLGTAAGNILVCLAIAWERRLQNVTNYFLMSLAITDLMVAVLVM 127
            .||   ..|     |:...:.::.|..|||||.||...:|.::..:|||:.|||.||:::..:.|
  Fly   126 PPF---ALWQTILIAICLAICIILTVGGNILVLLAFIVDRNIRQPSNYFIASLAATDMLIGTVSM 187

  Fly   128 PLGILTLVKGYFPLGSEHCLTWICLDVLFCTASIMHLCTISVDRYLSLRYPMRFGRNKTRRRVTL 192
            |...:.::|||:.||...|..|:.:|...|..|...:..|::|||.|::...::...:||.||..
  Fly   188 PFYTIYVLKGYWDLGPMLCDLWLSVDYTVCLVSQYTVLLITIDRYCSVKIAAKYRSWRTRTRVIY 252

  Fly   193 KIVFVWLLSIAMSLPLSLMYSKNHAS---VLVNGTCQI---PDPVYK---LVG-----SIVCFYI 243
            .:...|::. |:...:|: :...|.:   .|:.|.|.:   .||::.   ::|     .||.|.:
  Fly   253 MVTITWIIP-ALLFFISI-FGWEHFTGKRDLLPGQCAVQFLKDPIFNTALIIGYYWTTLIVLFVL 315

  Fly   244 PLGVMLLTYCLTVRLLARQRQNLGGGQQTAAATPGWASGWLGQAPA------------------- 289
            ..|:....|.:..|..|:||:.    |...|.:.|..||..|.|..
  Fly   316 YAGIYKTAYDMQKRSEAKQRKM----QSMVALSAGAMSGMAGHAAGIGVIEEKILKTKVELAGDQ 376

  Fly   290 --LERRCTW--RRLLKPGPGNASSVL------------HAHSAN----------STDTDLS---- 324
              |:..||.  :||...|..|..:|.            .|.:|.          :.|.:.|
  Fly   377 TDLDSACTTVIKRLSGSGQANPLAVATEEVEKMTPEQRRASAAKIQEEAKKREAAVDAEKSERSS 441

  Fly   325 --TLDNHELWLPDSSIKEP----TPTTMTALHQ-------FGAE-MLKLSRGLESVASSSTTGSP 375
              ..|:.|    :||:.:.    |...:..:.:       |||: .|..:||..::..|:|....
  Fly   442 SPAFDSDE----ESSVNQAQQLITQQKLNNMRKRSSIGLVFGAQAALLATRGKGNLQKSTTNSKS 502

  Fly   376 TKSEFSLSNHLQLQYPSSPQRYAATHQQS---HHHNHQQHPAGVYHQ-QASPKGRHGTTVLGLST 436
            .::.....:|.|..:..||.:.|.:.::.   |||.:||.|...:|| |.:|.
  Fly   503 IEAMHQYHHHQQHHHNQSPLQRAQSKEEMRSLHHHQNQQQPHHQHHQNQPNPP------------ 555

  Fly   437 TTLGIERESTRNSLASSRMGGEQSDGTLSQLSQRLRAYKKRRRASSAVPGREKRAGHEDADEDVE 501
                ::|..            ..|..||||:::                        .|...|:.
  Fly   556 ----LDRPK------------RTSCSTLSQIAE------------------------HDRLVDLS 580

  Fly   502 TPTSTLRRHKRHNSLPKNALYPRHTTVQESLDDDDDEEEESAQQKDQQTKSKICHSDTELDPPQK 566
            .|.:.|           |...|........::...|:..:...|             |.|.||
  Fly   581 APPTLL-----------NTSDPLSPVDLAPIEVPSDQVHQGLVQ-------------TILPPP-- 619

  Fly   567 AKQCHIAGQNHQSDYLQLPSVCTCPYFGDRPLQNCVKSAEVKII--------------SSAFRVT 617
                         |..|.|:..:..| .|||..|...::|:.:.              |||...:
  Fly   620 -------------DAFQCPTPLSDDY-SDRPFGNSSGNSELALTYDLMTNSELRYMDESSAMLAS 670

  Fly   618 TTTTAVSSSPSEME-----LLMCSSGNKKSLTSSVSAGITGGPAGAAPATVGSTLSPHSAHNQGS 677
            .|..:.:|....::     |.......:....|:::..::..|:.....:...:|:.:..|||..
  Fly   671 VTANSTTSPNDSVQGKPPVLPPPPPARRNPPKSNLNQSLSQTPSQNQSPSQSLSLNLNPNHNQSQ 735

  Fly   678 SLTVQS---------------------------------DGSGYLAAPGTPCPGRRKLSISKTAS 709
            |.:::.                                 |..|.|||   .||.||..|..:|:|
  Fly   736 SQSIEEAVAIEKRLLVSYTGIEDFAKVRRESCIEAICLLDVPGKLAA---DCPHRRASSPMETSS 797

  Fly   710 -----------------VVTWDSSRHRRRGSSFGGVRTSLLLTPTKTATTSNSSTPLRRSATLRS 757
                             |.|  |:.......|..||...:.|...:...||:|:|....|.:..:
  Fly   798 KDAILYSPMPPMPLSPKVKT--STPQTGTPQSAAGVTPPVPLERIEERETSDSNTNKMPSTSGTT 860

  Fly   758 HQNMNYQGAGECGGKTRTTTSSPCMLQRQQTVRSHHSRNSSVISRNSSRH------GRIIRLEQK 816
            .......|.||..|...|..::       ....::....|...|||||:.      |:..:.::.
  Fly   861 SSTGGVGGGGEQDGVGATKKNA-------GDDDANREEKSLAASRNSSKRAFIHSIGKHFKSKKA 918

  Fly   817 ATKVLGV---------------------VFFTFVILWSPFFVLNLLPTVCAE--CEERISHWVFD 858
            ...:|||                     :...||..|:|:.||.|:...|..  |   |:..::.
  Fly   919 LPLILGVGGRQKSKSENRARKAFRTISFILGCFVACWTPYHVLALVEGFCRHPPC---INEHLYM 980

  Fly   859 VVTWLGYASSMVNPIFYTIFNKVFRQAFKKVL 890
            ...:|.||:|.:||..|.:.|:.|::.|.::|
  Fly   981 FSYFLCYANSPMNPFCYALANQQFKKTFTRIL 1012

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
5-HT2B	NP_001262373.1	7tmA_5-HT2_insect-like	74..>267	CDD:320433	59/211 (28%)
		TM helix 1	75..101	CDD:320433	10/30 (33%)
		TM helix 2	108..134	CDD:320433	10/25 (40%)
		TM helix 3	146..176	CDD:320433	10/29 (34%)
		TM helix 4	188..211	CDD:320433	5/22 (23%)
		TM helix 5	229..258	CDD:320433	8/36 (22%)
		7tm_GPCRs	<799..893	CDD:475119	30/121 (25%)
		TM helix 6	818..840	CDD:410628	9/42 (21%)
		TM helix 7	854..879	CDD:410628	7/24 (29%)
mAChR-B	NP_649764.1	7tmA_mAChR_GAR-2-like	147..>323	CDD:320429	52/177 (29%)
		TM helix 2	168..194	CDD:320429	10/25 (40%)
		TM helix 3	206..236	CDD:320429	10/29 (34%)
		TM helix 4	248..271	CDD:320429	5/24 (21%)
		TM helix 5	293..322	CDD:320429	7/28 (25%)
		7tm_GPCRs	<932..1008	CDD:475119	19/78 (24%)
		TM helix 6	941..963	CDD:410628	6/21 (29%)
		TM helix 7	976..1001	CDD:410628	7/24 (29%)