DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment 5-HT2B and HTR2C

DIOPT Version :9

Sequence 1:	NP_001262373.1	Gene:	5-HT2B / 41017	FlyBaseID:	FBgn0261929	Length:	947	Species:	Drosophila melanogaster
Sequence 2:	NP_000859.2	Gene:	HTR2C / 3358	HGNCID:	5295	Length:	458	Species:	Homo sapiens

Alignment Length:	888	Identity:	166/888 - (18%)
Similarity:	245/888 - (27%)	Gaps:	511/888 - (57%)

- Green bases have known domain annotations that are detailed below.

  Fly    26 LVLWEED-ETQRTTANATSRHNQLHVARWNATGNATISATFEDVPFDANNYWALLALVLVLGTAA 89
            |::|:.| ......|..|...|.....|:.             .|....|:.||..:::::.|..
Human    18 LLVWQSDISVSPVAAIVTDIFNTSDGGRFK-------------FPDGVQNWPALSIVIIIIMTIG 69

  Fly    90 GNILVCLAIAWERRLQNVTNYFLMSLAITDLMVAVLVMPLGILTLVKGY-FPLGSEHCLTWICLD 153
            |||||.:|::.|::|.|.||||||||||.|::|.:|||||.:|.::..| :||....|..||.||
Human    70 GNILVIMAVSMEKKLHNATNYFLMSLAIADMLVGLLVMPLSLLAILYDYVWPLPRYLCPVWISLD 134

  Fly   154 VLFCTASIMHLCTISVDRYLSLRYPMRFGRNKTRRRVTLKIVFVWLLSIAMSLPLSLMYSKNHAS 218
            |||.||||||||.||:|||:::|.|:...|..:|.:..:||..||.:||.:|:|:.::..::...
Human   135 VLFSTASIMHLCAISLDRYVAIRNPIEHSRFNSRTKAIMKIAIVWAISIGVSVPIPVIGLRDEEK 199

  Fly   219 VLVNG-TCQIPDPVYKLVGSIVCFYIPLGVMLLTYCLTVRLLARQRQNLGGGQQTAAATPGWASG 282
            |.||. ||.:.||.:.|:||.|.|:|||.:|::|||||:.:|.||
Human   200 VFVNNTTCVLNDPNFVLIGSFVAFFIPLTIMVITYCLTIYVLRRQ-------------------- 244

  Fly   283 WLGQAPALERRCTWRRLLKPGPGNASSVLHAHSANSTDTDLSTLDNHELWLPDSSIKEPTPTTMT 347
                                    |..:||.|:                       :||.
Human   245 ------------------------ALMLLHGHT-----------------------EEPP----- 257

  Fly   348 ALHQFGAEMLKLSRGLESVASSSTTGSPTKSEFSLSNHLQLQYPSSPQRYAATHQQSHHHNHQQH 412

Human   258 ----------------------------------------------------------------- 257

  Fly   413 PAGVYHQQASPKGRHGTTVLGLSTTTLGIERESTRNSLASSRMGGEQSDGTLSQLSQRLRAYKKR 477
                                |||...|...:.:|.
Human   258 --------------------GLSLDFLKCCKRNTA------------------------------ 272

  Fly   478 RRASSAVPGREKRAGHEDADEDVETPTSTLRRHKRHNSLPKNALYPRHTTVQESLDDDDDEEEES 542
                                                                        |||.|
Human   273 ------------------------------------------------------------EEENS 277

  Fly   543 AQ-QKDQQTKSKICHSDTELDPPQKAKQCHIAGQNHQSDYLQLPSVCTCPYFGDRPLQNCVKSAE 606
            |. .:||..:
Human   278 ANPNQDQNAR------------------------------------------------------- 287

  Fly   607 VKIISSAFRVTTTTTAVSSSPSEMELLMCSSGNKKSLTSSVSAGITGGPAGAAPATVGSTLSPHS 671

Human   288 ----------------------------------------------------------------- 287

  Fly   672 AHNQGSSLTVQSDGSGYLAAPGTPCPGRRKLSISKTASVVTWDSSRHRRRGSSFGGVRTSLLLTP 736
                                       |||             ....|.||
Human   288 ---------------------------RRK-------------KKERRPRG-------------- 298

  Fly   737 TKTATTSNSSTPLRRSATLRSHQNMNYQGAGECGGKTRTTTSSPCMLQRQQTVRSHHSRNSSVIS 801
                             |:::..|
Human   299 -----------------TMQAINN----------------------------------------- 305

  Fly   802 RNSSRHGRIIRLEQKATKVLGVVFFTFVILWSPFFVLNLLPTVCAE-CEERISHWVFDVVTWLGY 865
                        |:||:||||:|||.|:|:|.|||:.|:|..:|.: |.:::...:.:|..|:||
Human   306 ------------ERKASKVLGIVFFVFLIMWCPFFITNILSVLCEKSCNQKLMEKLLNVFVWIGY 358

  Fly   866 ASSMVNPIFYTIFNKVFRQAFKKVLLCRYSSTSAWRPSRXQTP 908
            ..|.:||:.||:|||::|:||...|.|.|....  :|.. |.|
Human   359 VCSGINPLVYTLFNKIYRRAFSNYLRCNYKVEK--KPPVRQIP 399

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
5-HT2B	NP_001262373.1	7tm_1	90..>262	CDD:278431	85/173 (49%)
5-HT2B	NP_001262373.1	7tm_4	90..>238	CDD:304433	72/149 (48%)
HTR2C	NP_000859.2	7tmA_5-HT2C	54..385	CDD:341346	153/821 (19%)
		TM helix 1	56..80	CDD:341346	9/23 (39%)
		TM helix 2	89..111	CDD:341346	16/21 (76%)
		TM helix 3	128..150	CDD:341346	16/21 (76%)
		Agonist binding. /evidence=ECO:0000250	134..139		4/4 (100%)
		DRY motif, important for ligand-induced conformation changes. /evidence=ECO:0000250	151..153		1/1 (100%)
		TM helix 4	173..189	CDD:341346	7/15 (47%)
		TM helix 5	212..235	CDD:341346	11/22 (50%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	274..301		13/217 (6%)
		TM helix 6	310..332	CDD:341346	13/21 (62%)
		Agonist binding. /evidence=ECO:0000250	324..328		2/3 (67%)
		TM helix 7	347..372	CDD:341346	9/24 (38%)
		NPxxY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000250	364..368		2/3 (67%)
		PDZ-binding	456..458
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	252	1.000	Domainoid score	I2094
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	287	1.000	Inparanoid score	I2841
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D408957at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm41384
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	LDO	PTHR24247
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X759
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	8	8.070