DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment 5-HT2B and Htr2a

DIOPT Version :9

Sequence 1:	NP_001262373.1	Gene:	5-HT2B / 41017	FlyBaseID:	FBgn0261929	Length:	947	Species:	Drosophila melanogaster
Sequence 2:	NP_058950.1	Gene:	Htr2a / 29595	RGDID:	61800	Length:	471	Species:	Rattus norvegicus

Alignment Length:	886	Identity:	167/886 - (18%)
Similarity:	243/886 - (27%)	Gaps:	518/886 - (58%)

- Green bases have known domain annotations that are detailed below.

  Fly    12 YNDSGGDDWSSSEHLVLWEEDETQRTTANATSRHNQLHVARWNATGNATISAT-FEDVPFDANNY 75
            :||....|.::|| ...|..|...||                |.:....:..| ...:.....|:
  Rat    29 HNDFNSRDANTSE-ASNWTIDAENRT----------------NLSCEGYLPPTCLSILHLQEKNW 76

  Fly    76 WALLALVLVLGTAAGNILVCLAIAWERRLQNVTNYFLMSLAITDLMVAVLVMPLGILTLVKGY-F 139
            .|||..|:::.|.||||||.:|::.|::|||.||||||||||.|:::..||||:.:||::.|| :
  Rat    77 SALLTTVVIILTIAGNILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSMLTILYGYRW 141

  Fly   140 PLGSEHCLTWICLDVLFCTASIMHLCTISVDRYLSLRYPMRFGRNKTRRRVTLKIVFVWLLSIAM 204
            ||.|:.|..||.|||||.||||||||.||:|||::::.|:...|..:|.:..|||:.||.:|:.:
  Rat   142 PLPSKLCAIWIYLDVLFSTASIMHLCAISLDRYVAIQNPIHHSRFNSRTKAFLKIIAVWTISVGI 206

  Fly   205 SLPLSLMYSKNHASVLVNGTCQIPDPVYKLVGSIVCFYIPLGVMLLTYCLTVRLLARQRQNLGGG 269
            |:|:.:...::.:.|...|:|.:.|..:.|:||.|.|:|||.:|::||.||::.|.::
  Rat   207 SMPIPVFGLQDDSKVFKEGSCLLADDNFVLIGSFVAFFIPLTIMVITYFLTIKSLQKE------- 264

  Fly   270 QQTAAATPGWASGWLGQAPALERRCTWRRLLKPGPGNASSVLHAHSANSTDTDLSTLDNHELWLP 334

  Rat   265 ----------------------------------------------------------------- 264

  Fly   335 DSSIKEPTPTTMTALHQFGAEMLKLSRGLESVASSSTTGSPTKSEFSLSNHLQLQYPSSPQRYAA 399

  Rat   265 ----------------------------------------------------------------- 264

  Fly   400 THQQSHHHNHQQHPAGVYHQQASPKGRHGTTVLGLSTTTLGIERESTRNSLASSRMGGEQSDGTL 464
                                                 .||.:...|||..|||           .
  Rat   265 -------------------------------------ATLCVSDLSTRAKLAS-----------F 281

  Fly   465 SQLSQRLRAYKKRRRASSAVPGREKRAGHEDADEDVETPTSTLRRHKRHNSLPKNALYPRHTTVQ 529
            |.|.|                                            :||....|:.|
  Rat   282 SFLPQ--------------------------------------------SSLSSEKLFQR----- 297

  Fly   530 ESLDDDDDEEEESAQQKDQQTKSKICHSDTELDPPQKAKQCHIAGQNHQSDYLQLPSVCTCPYFG 594

  Rat   298 ----------------------------------------------------------------- 297

  Fly   595 DRPLQNCVKSAEVKIISSAFRVTTTTTAVSSSPSEMELLMCSSGNKKSLTSSVSAGITGGPAGAA 659

  Rat   298 ----------------------------------------------------------------- 297

  Fly   660 PATVGSTLSPHSAHNQGSSLTVQSDGSGYLAAPGTPCPGRRKLSISKTASVVTWDSSRHRRRGSS 724
                                                                    |.||..||.
  Rat   298 --------------------------------------------------------SIHREPGSY 306

  Fly   725 FGGVRTSLLLTPTKTATTSNSSTPLRRSATLRSHQNMNYQGAGECGGKTRTTTSSPCMLQRQQTV 789
            .|                       ||  |::|..|
  Rat   307 AG-----------------------RR--TMQSISN----------------------------- 317

  Fly   790 RSHHSRNSSVISRNSSRHGRIIRLEQKATKVLGVVFFTFVILWSPFFVLNLLPTVCAE-CEERIS 853
                                    ||||.||||:|||.||::|.|||:.|::..:|.| |.|.:.
  Rat   318 ------------------------EQKACKVLGIVFFLFVVMWCPFFITNIMAVICKESCNENVI 358

  Fly   854 HWVFDVVTWLGYASSMVNPIFYTIFNKVFRQAFKKVLLCRY 894
            ..:.:|..|:||.||.|||:.||:|||.:|.||.:.:.|:|
  Rat   359 GALLNVFVWIGYLSSAVNPLVYTLFNKTYRSAFSRYIQCQY 399

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
5-HT2B	NP_001262373.1	7tm_1	90..>262	CDD:278431	82/172 (48%)
5-HT2B	NP_001262373.1	7tm_4	90..>238	CDD:304433	70/148 (47%)
Htr2a	NP_058950.1	7tmA_5-HT2A	75..391	CDD:341345	152/813 (19%)
		TM helix 1	77..101	CDD:341345	12/23 (52%)
		TM helix 2	110..132	CDD:341345	14/21 (67%)
		TM helix 3	149..171	CDD:341345	16/21 (76%)
		Agonist binding. /evidence=ECO:0000250\|UniProtKB:P41595	155..160		4/4 (100%)
		DRY motif, important for ligand-induced conformation changes. /evidence=ECO:0000250\|UniProtKB:P41595	172..174		1/1 (100%)
		TM helix 4	194..210	CDD:341345	7/15 (47%)
		TM helix 5	232..255	CDD:341345	10/22 (45%)
		TM helix 6	322..344	CDD:341345	13/21 (62%)
		Agonist binding. /evidence=ECO:0000250\|UniProtKB:P41595	336..340		2/3 (67%)
		TM helix 7	359..384	CDD:341345	11/24 (46%)
		NPxxY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000250\|UniProtKB:P41595	376..380		2/3 (67%)
		PDZ-binding. /evidence=ECO:0000250\|UniProtKB:P28223	469..471
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	253	1.000	Domainoid score	I1997
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	287	1.000	Inparanoid score	I2747
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D408957at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_109260
Panther	1	1.100	-	-	O	PTHR24247
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X759
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	9	8.970