Protein Alignment 5-HT2B and Htr2a

DIOPT Version :10

• Sequence 1: NP_001262373.1
• Gene: 5-HT2B / 41017
• FlyBaseID: FBgn0261929
• Length: 947
• Species: Drosophila melanogaster
• Sequence 2: NP_058950.1
• Gene: Htr2a / 29595
• RGDID: 61800
• Length: 471
• Species: Rattus norvegicus
• Alignment Length: 886
• Identity: 167/886 - (18%)
• Similarity: 243/886 - (27%)
• Gaps: 518/886 - (58%)

  Fly    12 YNDSGGDDWSSSEHLVLWEEDETQRTTANATSRHNQLHVARWNATGNATISAT-FEDVPFDANNY 75
            :||....|.::|| ...|..|...||                |.:....:..| ...:.....|:
  Rat    29 HNDFNSRDANTSE-ASNWTIDAENRT----------------NLSCEGYLPPTCLSILHLQEKNW 76

  Fly    76 WALLALVLVLGTAAGNILVCLAIAWERRLQNVTNYFLMSLAITDLMVAVLVMPLGILTLVKGY-F 139
            .|||..|:::.|.||||||.:|::.|::|||.||||||||||.|:::..||||:.:||::.|| :
  Rat    77 SALLTTVVIILTIAGNILVIMAVSLEKKLQNATNYFLMSLAIADMLLGFLVMPVSMLTILYGYRW 141

  Fly   140 PLGSEHCLTWICLDVLFCTASIMHLCTISVDRYLSLRYPMRFGRNKTRRRVTLKIVFVWLLSIAM 204
            ||.|:.|..||.|||||.||||||||.||:|||::::.|:...|..:|.:..|||:.||.:|:.:
  Rat   142 PLPSKLCAIWIYLDVLFSTASIMHLCAISLDRYVAIQNPIHHSRFNSRTKAFLKIIAVWTISVGI 206

  Fly   205 SLPLSLMYSKNHASVLVNGTCQIPDPVYKLVGSIVCFYIPLGVMLLTYCLTVRLLARQRQNLGGG 269
            |:|:.:...::.:.|...|:|.:.|..:.|:||.|.|:|||.:|::||.||::.|.::
  Rat   207 SMPIPVFGLQDDSKVFKEGSCLLADDNFVLIGSFVAFFIPLTIMVITYFLTIKSLQKE------- 264

  Fly   270 QQTAAATPGWASGWLGQAPALERRCTWRRLLKPGPGNASSVLHAHSANSTDTDLSTLDNHELWLP 334
                                                                             
  Rat   265 ----------------------------------------------------------------- 264

  Fly   335 DSSIKEPTPTTMTALHQFGAEMLKLSRGLESVASSSTTGSPTKSEFSLSNHLQLQYPSSPQRYAA 399
                                                                             
  Rat   265 ----------------------------------------------------------------- 264

  Fly   400 THQQSHHHNHQQHPAGVYHQQASPKGRHGTTVLGLSTTTLGIERESTRNSLASSRMGGEQSDGTL 464
                                                 .||.:...|||..|||           .
  Rat   265 -------------------------------------ATLCVSDLSTRAKLAS-----------F 281

  Fly   465 SQLSQRLRAYKKRRRASSAVPGREKRAGHEDADEDVETPTSTLRRHKRHNSLPKNALYPRHTTVQ 529
            |.|.|                                            :||....|:.|
  Rat   282 SFLPQ--------------------------------------------SSLSSEKLFQR----- 297

  Fly   530 ESLDDDDDEEEESAQQKDQQTKSKICHSDTELDPPQKAKQCHIAGQNHQSDYLQLPSVCTCPYFG 594
                                                                             
  Rat   298 ----------------------------------------------------------------- 297

  Fly   595 DRPLQNCVKSAEVKIISSAFRVTTTTTAVSSSPSEMELLMCSSGNKKSLTSSVSAGITGGPAGAA 659
                                                                             
  Rat   298 ----------------------------------------------------------------- 297

  Fly   660 PATVGSTLSPHSAHNQGSSLTVQSDGSGYLAAPGTPCPGRRKLSISKTASVVTWDSSRHRRRGSS 724
                                                                    |.||..||.
  Rat   298 --------------------------------------------------------SIHREPGSY 306

  Fly   725 FGGVRTSLLLTPTKTATTSNSSTPLRRSATLRSHQNMNYQGAGECGGKTRTTTSSPCMLQRQQTV 789
            .|                       ||  |::|..|
  Rat   307 AG-----------------------RR--TMQSISN----------------------------- 317

  Fly   790 RSHHSRNSSVISRNSSRHGRIIRLEQKATKVLGVVFFTFVILWSPFFVLNLLPTVCAE-CEERIS 853
                                    ||||.||||:|||.||::|.|||:.|::..:|.| |.|.:.
  Rat   318 ------------------------EQKACKVLGIVFFLFVVMWCPFFITNIMAVICKESCNENVI 358

  Fly   854 HWVFDVVTWLGYASSMVNPIFYTIFNKVFRQAFKKVLLCRY 894
            ..:.:|..|:||.||.|||:.||:|||.:|.||.:.:.|:|
  Rat   359 GALLNVFVWIGYLSSAVNPLVYTLFNKTYRSAFSRYIQCQY 399

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
5-HT2B	NP_001262373.1	7tmA_5-HT2_insect-like	74..>267	CDD:320433	89/193 (46%)
		TM helix 1	75..101	CDD:320433	12/25 (48%)
		TM helix 2	108..134	CDD:320433	16/25 (64%)
		TM helix 3	146..176	CDD:320433	21/29 (72%)
		TM helix 4	188..211	CDD:320433	8/22 (36%)
		TM helix 5	229..258	CDD:320433	14/28 (50%)
		7tm_GPCRs	<799..893	CDD:475119	39/94 (41%)
		TM helix 6	818..840	CDD:410628	13/21 (62%)
		TM helix 7	854..879	CDD:410628	11/24 (46%)
Htr2a	NP_058950.1	7tmA_5-HT2A	75..391	CDD:341345	152/813 (19%)
		TM helix 1	76..102	CDD:341345	12/25 (48%)
		TM helix 2	109..135	CDD:341345	16/25 (64%)
		TM helix 3	148..178	CDD:341345	21/29 (72%)
		DRY motif, important for ligand-induced conformation changes. /evidence=ECO:0000250|UniProtKB:P41595	172..174		1/1 (100%)
		TM helix 4	190..213	CDD:341345	8/22 (36%)
		TM helix 5	231..260	CDD:341345	14/28 (50%)
		TM helix 6	316..346	CDD:341345	19/82 (23%)
		TM helix 7	359..384	CDD:341345	11/24 (46%)
		NPxxY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000250|UniProtKB:P41595	376..380		2/3 (67%)
		PDZ-binding. /evidence=ECO:0000250|UniProtKB:P28223	469..471