Protein Alignment 5-HT2B and Htr1d

DIOPT Version :10

• Sequence 1: NP_001262373.1
• Gene: 5-HT2B / 41017
• FlyBaseID: FBgn0261929
• Length: 947
• Species: Drosophila melanogaster
• Sequence 2: NP_036984.1
• Gene: Htr1d / 25323
• RGDID: 2847
• Length: 374
• Species: Rattus norvegicus
• Alignment Length: 860
• Identity: 134/860 - (15%)
• Similarity: 200/860 - (23%)
• Gaps: 519/860 - (60%)

  Fly    64 TFEDVPFDANN-------YW---ALLAL---------VLVLGTAAGNILVCLAIAWERRLQNVTN 109
            :.|.:|.:|:|       .|   .|.||         ::.|.|...|..|...|...::|....|
  Rat     7 SLEGLPQEASNRSLNATGAWDPEVLQALRISLVVVLSIITLATVLSNAFVLTTILLTKKLHTPAN 71

  Fly   110 YFLMSLAITDLMVAVLVMPLGILTLVKGYFPLGSEHCLTWICLDVLFCTASIMHLCTISVDRYLS 174
            |.:.|||.|||:|::||||:.|.......:..|...|..|:..|:..|||||:|||.|::|||.:
  Rat    72 YLIGSLATTDLLVSILVMPISIAYTTTRTWNFGQILCDIWVSSDITCCTASILHLCVIALDRYWA 136

  Fly   175 LRYPMRFGRNKTRRRVTLKIVFVWLLSIAMSL-PLSLMYSKNH---ASVLVNGTCQIPDPVYKLV 235
            :...:.:.:.:|.......|..||.:||.:|: ||....:..|   :..||| |.||...:|...
  Rat   137 ITDALEYSKRRTAGHAAAMIAAVWAISICISIPPLFWRQATAHEEMSDCLVN-TSQISYTIYSTC 200

  Fly   236 GSIVCFYIPLGVMLLTYCLTVRLLARQRQNLGGGQQTAAATPGWASGWLGQAPALERRCTWRRLL 300
            |:   ||||..::::.|                |:...||.                    .|:|
  Rat   201 GA---FYIPSILLIILY----------------GRIYVAAR--------------------SRIL 226

  Fly   301 KPGPGNASSVLHAHSANSTDTDLSTLDNHELWLPDSSIKEPTPTTMTALHQFGAEMLKLSRGLES 365
            .|
  Rat   227 NP--------------------------------------------------------------- 228

  Fly   366 VASSSTTGSPTKSEFSLSNHLQLQYPSSPQRYAATHQQSHHHNHQQHPAGVYHQQASPKGRHGTT 430
                                        |..|.
  Rat   229 ----------------------------PSLYG-------------------------------- 233

  Fly   431 VLGLSTTTLGIERESTRNSLASSRMGGEQSDGTLSQLSQRLRAYKKRRRASSAVPGREKRAGHED 495
                                                                             
  Rat   234 ----------------------------------------------------------------- 233

  Fly   496 ADEDVETPTSTLRRHKRHNSLPKNALYPRHTTVQESLDDDDDEEEESAQQKDQQTKSKICHSDTE 560
                                       .|.||.|
  Rat   234 ---------------------------KRFTTAQ------------------------------- 240

  Fly   561 LDPPQKAKQCHIAGQNHQSDYLQLPSVCTCPYFGDRPLQNCVKSAEVKIISSAFRVTTTTTAVSS 625
                                                                             
  Rat   241 ----------------------------------------------------------------- 240

  Fly   626 SPSEMELLMCSSGNKKSLTSSVSAGITGGPAGAAPATVGSTLSPHSAHNQGSSLTVQSDGSGYLA 690
                                     :..|.||::..::..:|.....|..||.|
  Rat   241 -------------------------LITGSAGSSLCSLNPSLHESHTHTVGSPL----------- 269

  Fly   691 APGTPCPGRRKLSISKTASVVTWDSSRHRRRGSSFGGVRTSLLLTPTKTATTSNSSTPLRRSATL 755
                                             .|..|:..|.
  Rat   270 ---------------------------------FFNQVKIKLA---------------------- 279

  Fly   756 RSHQNMNYQGAGECGGKTRTTTSSPCMLQRQQTVRSHHSRNSSVISRNSSRHGRIIRLEQKATKV 820
                                                     .|::.|......|    |:||||.
  Rat   280 -----------------------------------------DSILERKRISAAR----ERKATKT 299

  Fly   821 LGVVFFTFVILWSPFFVLNLLPTVCAECEERISHWV----FDVVTWLGYASSMVNPIFYTIFNKV 881
            ||::...|:|.|.||||::|:..:|     |.|.|:    ||..|||||.:|::||:.||:||:.
  Rat   300 LGIILGAFIICWLPFFVVSLVLPIC-----RDSCWIHPALFDFFTWLGYLNSLINPVIYTVFNED 359

  Fly   882 FRQAFKKVLLCRYSS 896
            |||||::|:..|.:|
  Rat   360 FRQAFQRVVHFRKAS 374

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
5-HT2B	NP_001262373.1	7tmA_5-HT2_insect-like	74..>267	CDD:320433	64/215 (30%)
		TM helix 1	75..101	CDD:320433	9/37 (24%)
		TM helix 2	108..134	CDD:320433	14/25 (56%)
		TM helix 3	146..176	CDD:320433	15/29 (52%)
		TM helix 4	188..211	CDD:320433	8/23 (35%)
		TM helix 5	229..258	CDD:320433	7/28 (25%)
		7tm_GPCRs	<799..893	CDD:475119	41/97 (42%)
		TM helix 6	818..840	CDD:410628	11/21 (52%)
		TM helix 7	854..879	CDD:410628	13/28 (46%)
Htr1d	NP_036984.1	7tmA_5-HT1B_1D	32..364	CDD:320455	123/823 (15%)
		TM helix 1	37..63	CDD:320455	5/25 (20%)
		TM helix 2	70..96	CDD:320455	14/25 (56%)
		TM helix 3	108..138	CDD:320455	15/29 (52%)
		DRY motif, important for ligand-induced conformation changes. /evidence=ECO:0000250|UniProtKB:P41595	132..134		1/1 (100%)
		TM helix 4	150..173	CDD:320455	8/22 (36%)
		TM helix 5	191..220	CDD:320455	10/47 (21%)
		TM helix 6	291..321	CDD:320455	16/33 (48%)
		TM helix 7	332..357	CDD:320455	12/24 (50%)
		NPxxY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000250|UniProtKB:P41595	349..353		2/3 (67%)