DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment 5-HT2B and Htr2c

DIOPT Version :9

Sequence 1:	NP_001262373.1	Gene:	5-HT2B / 41017	FlyBaseID:	FBgn0261929	Length:	947	Species:	Drosophila melanogaster
Sequence 2:	NP_032338.3	Gene:	Htr2c / 15560	MGIID:	96281	Length:	459	Species:	Mus musculus

Alignment Length:	856	Identity:	159/856 - (18%)
Similarity:	239/856 - (27%)	Gaps:	495/856 - (57%)

- Green bases have known domain annotations that are detailed below.

  Fly    56 TGNATISATFEDVPFDANNYWALLALVLVLGTAAGNILVCLAIAWERRLQNVTNYFLMSLAITDL 120
            |.|::........|....|:.||..:|:::.|..|||||.:|::.|::|.|.||||||||||.|:
Mouse    37 TFNSSDGGRLFQFPDGVQNWPALSIVVIIIMTIGGNILVIMAVSMEKKLHNATNYFLMSLAIADM 101

  Fly   121 MVAVLVMPLGILTLVKGY-FPLGSEHCLTWICLDVLFCTASIMHLCTISVDRYLSLRYPMRFGRN 184
            :|.:|||||.:|.::..| :||....|..||.|||||.||||||||.||:|||:::|.|:...|.
Mouse   102 LVGLLVMPLSLLAILYDYVWPLPRYLCPVWISLDVLFSTASIMHLCAISLDRYVAIRNPIEHSRF 166

  Fly   185 KTRRRVTLKIVFVWLLSIAMSLPLSLMYSKNHASVLVNG-TCQIPDPVYKLVGSIVCFYIPLGVM 248
            .:|.:..:||..||.:||.:|:|:.::..::.:.|.||. ||.:.||.:.|:||.|.|:|||.:|
Mouse   167 NSRTKAIMKIAIVWAISIGVSVPIPVIGLRDESKVFVNNTTCVLNDPNFVLIGSFVAFFIPLTIM 231

  Fly   249 LLTYCLTVRLLARQRQNLGGGQQTAAATPGWASGWLGQAPALERRCTWRRLLKPGPGNASSVLHA 313
            ::||.||:.:|.||
Mouse   232 VITYFLTIYVLRRQ--------------------------------------------------- 245

  Fly   314 HSANSTDTDLSTLDNHELWLPDSSIKEPTPTTMTALHQFGAEMLKLSRGLESVASSSTTGSPTKS 378
                                                      .|.|.||
Mouse   246 ------------------------------------------TLMLLRG---------------- 252

  Fly   379 EFSLSNHLQLQYPSSPQRYAATHQQSHHHNHQQHPAGVYHQQASPKGRHGTTVLGLSTTTLGIER 443

Mouse   253 ----------------------------------------------------------------- 252

  Fly   444 ESTRNSLASSRMGGEQSDGTLSQLSQRLRAYKKRRRASSAVPGREKRAGHEDADEDVETPTSTLR 508

Mouse   253 ----------------------------------------------------------------- 252

  Fly   509 RHKRHNSLPKNALYPRHTTVQESLDDDDDEEEESAQQKDQQTKSKICHSDTELDPPQKAKQCHIA 573
                                                           |::.||
Mouse   253 -----------------------------------------------HTEEEL------------ 258

  Fly   574 GQNHQSDYLQLPSVCTCPYFGDRPLQNCVKSAEVKIISSAFRVTTTTTAVSSSPSEMELLMCSSG 638
             :|...::|:    |.|.. ||.                      ...|.:.:|.:.
Mouse   259 -RNISLNFLK----CCCKK-GDE----------------------EENAPNPNPDQK-------- 287

  Fly   639 NKKSLTSSVSAGITGGPAGAAPATVGSTLSPHSAHNQGSSLTVQSDGSGYLAAPGTPCPGRRKLS 703
                                                                      |.|:|
Mouse   288 ----------------------------------------------------------PRRKK-- 292

  Fly   704 ISKTASVVTWDSSRHRRRGSSFGGVRTSLLLTPTKTATTSNSSTPLRRSATLRSHQNMNYQGAGE 768
                        ...|.||                               |:::..|
Mouse   293 ------------KEKRPRG-------------------------------TMQAINN-------- 306

  Fly   769 CGGKTRTTTSSPCMLQRQQTVRSHHSRNSSVISRNSSRHGRIIRLEQKATKVLGVVFFTFVILWS 833
                                                         |:||:||||:|||.|:|:|.
Mouse   307 ---------------------------------------------EKKASKVLGIVFFVFLIMWC 326

  Fly   834 PFFVLNLLPTVCAE-CEERISHWVFDVVTWLGYASSMVNPIFYTIFNKVFRQAFKKVLLCRYSST 897
            |||:.|:|..:|.: |.:::...:.:|..|:||..|.:||:.||:|||::|:||.|.|.|.|...
Mouse   327 PFFITNILSVLCGKACNQKLMEKLLNVFVWIGYVCSGINPLVYTLFNKIYRRAFSKYLRCDYKPD 391

  Fly   898 SAWRPSRXQTP 908
            .  :|.. |.|
Mouse   392 K--KPPVRQIP 400

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
5-HT2B	NP_001262373.1	7tm_1	90..>262	CDD:278431	84/173 (49%)
5-HT2B	NP_001262373.1	7tm_4	90..>238	CDD:304433	72/149 (48%)
Htr2c	NP_032338.3	7tmA_5-HT2C	55..386	CDD:320431	151/820 (18%)
		TM helix 1	56..82	CDD:320431	10/25 (40%)
		TM helix 2	89..115	CDD:320431	18/25 (72%)
		TM helix 3	128..158	CDD:320431	21/29 (72%)
		Agonist binding. /evidence=ECO:0000250	135..140		4/4 (100%)
		DRY motif, important for ligand-induced conformation changes. /evidence=ECO:0000250	152..154		1/1 (100%)
		TM helix 4	170..193	CDD:320431	8/22 (36%)
		TM helix 5	212..241	CDD:320431	15/28 (54%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	274..302		10/160 (6%)
		TM helix 6	305..335	CDD:320431	18/82 (22%)
		Agonist binding. /evidence=ECO:0000250	325..329		2/3 (67%)
		TM helix 7	348..373	CDD:320431	9/24 (38%)
		NPxxY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000250	365..369		2/3 (67%)
		PDZ-binding	457..459
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	253	1.000	Domainoid score	I2062
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	288	1.000	Inparanoid score	I2804
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D408957at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	LDO	PTHR24247
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X759
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	7	7.070