DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment pch2 and Spast

DIOPT Version :9

Sequence 1:	NP_001287235.1	Gene:	pch2 / 41013	FlyBaseID:	FBgn0051453	Length:	421	Species:	Drosophila melanogaster
Sequence 2:	NP_001156342.1	Gene:	Spast / 50850	MGIID:	1858896	Length:	614	Species:	Mus musculus

Alignment Length:	286	Identity:	79/286 - (27%)
Similarity:	128/286 - (44%)	Gaps:	45/286 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly   141 EKLLKFALSALMF---SEHRVDTNVIACNRLILLHGPPGTGKTSLCKALAQKLSIRTQGSYAYTH 202
            ::|.|.||..::.   ....:.|.:.|..|.:||.||||.|||.|.||:|.:         :...
Mouse   345 QELAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAE---------SNAT 400

  Fly   203 LVEINSHSLFSKWFSESGKLVAQLFNKIAELVSDPNNLVCVLIDEVESLAYARSAMSSNEPRDAM 267
            ...|::.||.||:..|..|||..||....||  .|:   .:.||||:||...|   ...|...:.
Mouse   401 FFNISAASLTSKYVGEGEKLVRALFAVAREL--QPS---IIFIDEVDSLLCER---REGEHDASR 457

  Fly   268 RVVNAVLTQLDSLKTC--PNVLILATSNLAQSIDLAFVDRADIRLFIGYPGISAIREIYKGMLAE 330
            |:....|.:.|.:::.  ..||::..:|..|.:|.|.:.|...|:::..|.......:.|.:|.:
Mouse   458 RLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCK 522

  Fly   331 LMSAGVLQREVLESEDAEEGLLTQLAERSVGLSGRTLRKL-------PL--LAHAQYTSSTLFEL 386
             ..:.:.|:|           |.|||..:.|.||..|..|       |:  |...|..:.:..|:
Mouse   523 -QGSPLTQKE-----------LAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEM 575

  Fly   387 DQKISLSDFLDAMLEALEQHLGEQRL 412
             :.|.||||.:: |:.:::.:..|.|
Mouse   576 -RNIRLSDFTES-LKKIKRSVSPQTL 599

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
pch2	NP_001287235.1	AAA	166..315	CDD:214640	47/150 (31%)
pch2	NP_001287235.1	AAA	169..314	CDD:278434	46/146 (32%)
Spast	NP_001156342.1	Required for interaction with RTN1. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	1..298
		Required for midbody localization. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	1..192
		Required for interaction with ATL1. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	1..78
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..48
		Required for nuclear localization. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	1..48
		Nuclear localization signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	48..85
		Nuclear export signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021	57..65
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	91..112
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	110..194
		Required for interaction with microtubules. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	112..198
		MIT_spastin	114..193	CDD:239142
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	220..306
		Sufficient for microtubule severing. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	226..614		79/286 (28%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	268..326
		Nuclear localization signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021	307..310
		Required for interaction with microtubules. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	308..310
		P-loop_NTPase	339..>395	CDD:304359	19/49 (39%)
		AAA	372..508	CDD:214640	47/152 (31%)
		AAA	376..506	CDD:278434	46/146 (32%)
		Vps4_C	<578..610	CDD:286426	8/23 (35%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0464
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900