DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment RpA-70 and RFA1

DIOPT Version :9

Sequence 1:	NP_524274.1	Gene:	RpA-70 / 40972	FlyBaseID:	FBgn0010173	Length:	603	Species:	Drosophila melanogaster
Sequence 2:	NP_009404.1	Gene:	RFA1 / 851266	SGDID:	S000000065	Length:	621	Species:	Saccharomyces cerevisiae

Alignment Length:	604	Identity:	196/604 - (32%)
Similarity:	332/604 - (54%)	Gaps:	46/604 - (7%)

- Green bases have known domain annotations that are detailed below.

  Fly    24 VLQILAIKKINSAADSERYRILISDGKYFNSYAMLASQLNVMQHNGELEEFTIVQLDKYVTSLVG 88
            |.|:...:|.:.|..:.:..|:||||.| :..|:|.:|......:.||:...|:::.....::|.
Yeast    28 VYQVYNTRKSDGANSNRKNLIMISDGIY-HMKALLRNQAASKFQSMELQRGDIIRVIIAEPAIVR 91

  Fly    89 KDGAGKRVLIISELTVVNPGAEVKSKIGEPVTY----------ENAAKQDLAPKPAVT--SNSKP 141
            :  ..|.||::.:..:|...|::   :.:..|:          |....:|:.....|.  :|:..
Yeast    92 E--RKKYVLLVDDFELVQSRADM---VNQTSTFLDNYFSEHPNETLKDEDITDSGNVANQTNASN 151

  Fly   142 IAKKEPSHNNNN-----NIVMNSSINSGMTHP---ISSLSPYQNKWVIKARVTSKSGIRTWSNAR 198
            ....:..|:|:|     ....|.:.||..|.|   |..||||||.|.|||||:.|..|:||.|.|
Yeast   152 AGVPDMLHSNSNLNANERKFANENPNSQKTRPIFAIEQLSPYQNVWTIKARVSYKGEIKTWHNQR 216

  Fly   199 GEGKLFSMDLMDESGEIRATAFKEQCDKFYDLIQVDSVYYISKCQLKPANKQYSSLNNAYEMTFS 263
            |:||||:::.:|.|||||||||.:...||.:::|...|||:||.:|:||..|:::|.:.||:...
Yeast   217 GDGKLFNVNFLDTSGEIRATAFNDFATKFNEILQEGKVYYVSKAKLQPAKPQFTNLTHPYELNLD 281

  Fly   264 GETVVQLCEDTDDDPIPEIKYNLVPISDVSGMENKAAVDTIGICKEVGELQSFVARTTNKEFKKR 328
            .:||::.|  .|:..:|:..:|.:.:..:...|..:.||.:||.:.:.......:| ..|:|.:|
Yeast   282 RDTVIEEC--FDESNVPKTHFNFIKLDAIQNQEVNSNVDVLGIIQTINPHFELTSR-AGKKFDRR 343

  Fly   329 DITLVDMSNSAISLTLWGDDAVNFDGHVQPVILVKGTRINEFNGGKSLSLGGGSIMKINPDIPEA 393
            |||:||.|..:||:.||...|::|:.....|..:||.|:.:| ||||||:|..|.:..||:||||
Yeast   344 DITIVDDSGFSISVGLWNQQALDFNLPEGSVAAIKGVRVTDF-GGKSLSMGFSSTLIPNPEIPEA 407

  Fly   394 HKLRGWFDNGGGDSVANMVSAR----TGGGS------FSTEWMTLKDARARNLGSGDKPDYFQCK 448
            :.|:||:|:.|.:  ||.::.:    .||.|      |..:.:|:..|:|.|||..:|.|:|..|
Yeast   408 YALKGWYDSKGRN--ANFITLKQEPGMGGQSAASLTKFIAQRITIARAQAENLGRSEKGDFFSVK 470

  Fly   449 AVVHIVKQENAFYRACPQSDCNKKVVDEGNDQFRCEKC--NALFPNFKYRLLINMSIGDWTSNRW 511
            |.:..:|.:|..|.||...:|||||:::.:..:|||||  |...||::|  ::.:||.|.|:..|
Yeast   471 AAISFLKVDNFAYPACSNENCNKKVLEQPDGTWRCEKCDTNNARPNWRY--ILTISIIDETNQLW 533

  Fly   512 VSSFNEVGEQLLGHTSQEVGEALENDPAKAEQIFSALNFTSHIFKLRCKNEVYGDMTRNKLTVQS 576
            ::.|::..:||||..:..:....|.||.:..:|..::....:.|::|.:.:.|.|.:|.:.||.:
Yeast   534 LTLFDDQAKQLLGVDANTLMSLKEEDPNEFTKITQSIQMNEYDFRIRAREDTYNDQSRIRYTVAN 598

  Fly   577 VAPINHKEYNKHLLKELQE 595
            :..:|::....:|..||.:
Yeast   599 LHSLNYRAEADYLADELSK 617

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
RpA-70	NP_524274.1	rpa1	3..595	CDD:273177	196/602 (33%)
		Rep-A_N	6..107	CDD:281980	21/82 (26%)
		RPA1_DBD_A	168..270	CDD:239920	52/104 (50%)
		RPA1_DBD_B	301..401	CDD:239921	42/99 (42%)
		Rep_fac-A_C	444..589	CDD:285809	44/146 (30%)
RFA1	NP_009404.1	rpa1	3..617	CDD:273177	196/602 (33%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157345360
Domainoid	1	1.000	92	1.000	Domainoid score	I1736
eggNOG	1	0.900	-	-		E1_COG1599
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2208
Inparanoid	1	1.050	316	1.000	Inparanoid score	I463
Isobase	1	0.950	-	0	Normalized mean entropy	S1600
OMA	1	1.010	-	-		QHG54397
OrthoFinder	1	1.000	-	-		FOG0002228
OrthoInspector	1	1.000	-	-		oto100192
orthoMCL	1	0.900	-	-		OOG6_101316
Panther	1	1.100	-	-	LDO	PTHR23273
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2748
SonicParanoid	1	1.000	-	-		X1901
TreeFam	1	0.960	-	-
	16	15.740