DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment mAChR-B and chrm2b

DIOPT Version :9

Sequence 1:	NP_649764.1	Gene:	mAChR-B / 40955	FlyBaseID:	FBgn0037546	Length:	1019	Species:	Drosophila melanogaster
Sequence 2:	XP_021326218.1	Gene:	chrm2b / 555516	ZFINID:	ZDB-GENE-090410-3	Length:	476	Species:	Danio rerio

Alignment Length:	919	Identity:	176/919 - (19%)
Similarity:	269/919 - (29%)	Gaps:	461/919 - (50%)

- Green bases have known domain annotations that are detailed below.

  Fly   110 TMSGSSSDSEIL-----GPVLPPFALWQTILIAICLAICIILTVGGNILVLLAFIVDRNIRQPSN 169
            |:|.|||.:...     ||..|.    :.:||.:.|:|..::|:.||:||:|:..|:||::..:|
Zfish     7 TLSPSSSHANKTNIFQDGPFKPV----EFVLIVLGLSILSLITIIGNVLVMLSIKVNRNLQTVNN 67

  Fly   170 YFIASLAATDMLIGTVSMPFYTIYVLKGYWDLGPMLCDLWLSVDYTVCLVSQYTVLLITIDRYCS 234
            ||:.|||..|:.||..||..||:|::.|:|.||.::|||||::||.|...|...:|:|:.|||..
Zfish    68 YFLFSLACADLFIGVFSMNLYTVYIVTGHWPLGALVCDLWLALDYVVSNASVMNLLIISFDRYFC 132

  Fly   235 VKIAAKYRSWRTRTRVIYMVTITWIIPALLFFISIFGWEHFTGKRDLLPGQCAVQFLKDPIFNTA 299
            |.....|...||......|:...||:..:|:..:|..|:..||.|.:..|:|.:||..:.:....
Zfish   133 VTKPLSYPVKRTHKMAGMMIAAAWILSFILWAPAILFWQFITGSRTVPEGECYIQFFSNAVVTFG 197

  Fly   300 LIIGYYWTTLIVLFVLYAGIYKTAYDMQKRSEAKQRKMQSMVALSAGAMSGMAGHAAGIGVIEEK 364
            ..|..::..:|.:.:||..|.|.:     ||..::|..:.:..:|..      |..||:|
Zfish   198 TAIAAFYLPVITMTILYWQISKAS-----RSRVRRRDNRRVSRVSND------GGQAGLG----- 246

  Fly   365 ILKTKVELAGDQTDLDSACTTVIKRLSGSGQANPLAVATEEVEKMTPEQRRASAAKIQEEAKKRE 429

Zfish   247 ----------------------------------------------------------------- 246

  Fly   430 AAVDAEKSERSSSPAFDSDEESSVNQAQQLITQQKLNNMRKRSSIGLVFGAQAALLATRGKGNLQ 494
                   :||                           ||.::|:
Zfish   247 -------NER---------------------------NMDRKST--------------------- 256

  Fly   495 KSTTNSKSIEAMHQYHHHQQHHHNQSPLQRAQSKEEMRSLHHHQNQQQPHHQHHQNQPNPPLDRP 559
                                           |..|||                        :.|.
Zfish   257 -------------------------------QEGEEM------------------------VSRK 266

  Fly   560 KRTSCSTLSQIAEHDRLVDLSAPPTLLNTSDPLSPVDLAPIEVPSDQVHQGLVQTILPPPDAFQC 624
            :..|.:|                                          .||..::.||
Zfish   267 QSASDTT------------------------------------------TGLKISLFPP------ 283

  Fly   625 PTPLSDDYSDRPFGNSSGNSELALTYDLMTNSELRYMDESSAMLASVTANSTTSPNDSVQGKPPV 689
                                                           ......|.|||:.|
Zfish   284 -----------------------------------------------AGEERDSENDSISG---- 297

  Fly   690 LPPPPPARRNPPKSNLNQSLSQTPSQNQSPSQSLSLNLNPNHNQSQSQSIEEAVAIEKRLLVSYT 754
                                |...|.||...:::|::.|.:....|:||
Zfish   298 --------------------SVLASSNQRDDEAVSISTNGDIRARQNQS---------------- 326

  Fly   755 GIEDFAKVRRESCIEAICLLDVPGKLAADCPHRRASSPMETSSKDAILYSPMPPMPLSPKVKTST 819
                 |.:..||.:...|.     |.|              |.||.
Zfish   327 -----ASLATESWVRFTCF-----KTA--------------SQKDL------------------- 348

  Fly   820 PQTGTPQSAAGVTPPVPLERIEERETSDSNTNKMPSTS-GTTSSTGGVGGGGEQDGVGATKKNAG 883
                                        .||.|:.|.| |.|:
Zfish   349 ----------------------------QNTYKVNSESQGMTN---------------------- 363

  Fly   884 DDDANREEKSLAASRNSSKRAFIHSIGKHFKSKKALPLILGVGGRQKSKSENRARKAFRTISFIL 948
                .:|..||.:.:         .|..|.:.:|:              |.:|.:|..|||..||
Zfish   364 ----GKERLSLVSQK---------IISPHSQKRKS--------------SSSREKKVTRTIMAIL 401

  Fly   949 GCFVACWTPYHVLALVEGFCRHPPCINEHLYMFSYFLCYANSPMNPFCYALANQQFKKTFTRILK 1013
            ..|...||||:|:.|:..||  ..||...:::|.|:|||.||.:||.||||.|..||.||.::|.
Zfish   402 VAFATTWTPYNVMVLINTFC--SDCIPNTVWIFGYWLCYINSTVNPACYALCNVTFKNTFKQLLT 464

  Fly  1014 ---GDLHMT 1019
               .::|.|
Zfish   465 CKYRNIHAT 473

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
mAChR-B	NP_649764.1	7tm_1	150..>341	CDD:278431	66/190 (35%)
mAChR-B	NP_649764.1	7tm_4	150..>336	CDD:304433	65/185 (35%)
chrm2b	XP_021326218.1	7tmA_mAChR_M2	32..459	CDD:320424	163/874 (19%)
		TM helix 1	33..59	CDD:320424	10/25 (40%)
		TM helix 2	66..92	CDD:320424	13/25 (52%)
		TM helix 3	104..134	CDD:320424	14/29 (48%)
		TM helix 4	146..169	CDD:320424	5/22 (23%)
		TM helix 5	191..220	CDD:320424	5/28 (18%)
		TM helix 6	264..418	CDD:320424	58/408 (14%)
		TM helix 7	427..452	CDD:320424	13/24 (54%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1245472at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	3	2.920