Protein Alignment mAChR-B and Chrm3

DIOPT Version :10

• Sequence 1: NP_649764.1
• Gene: mAChR-B / 40955
• FlyBaseID: FBgn0037546
• Length: 1019
• Species: Drosophila melanogaster
• Sequence 2: NP_036659.2
• Gene: Chrm3 / 24260
• RGDID: 2343
• Length: 589
• Species: Rattus norvegicus
• Alignment Length: 962
• Identity: 192/962 - (19%)
• Similarity: 303/962 - (31%)
• Gaps: 441/962 - (45%)

  Fly    63 EAAKIYGESSDFNSWPVNEQLQWYRNFDVNNTNLLLAVRNQSSD--GGSTMSGSSSDSEILGPVL 125
            ||....|..:...|:.::::.   .||..|:|         |||  ||.|
  Rat    26 EAGLPLGTVTQLGSYNISQET---GNFSSNDT---------SSDPLGGHT--------------- 63

  Fly   126 PPFALWQTILIAICLAICIILTVGGNILVLLAFIVDRNIRQPSNYFIASLAATDMLIGTVSMPFY 190
                :||.:.||.......::|:.|||||::||.|::.::..:|||:.|||..|::||.:||..:
  Rat    64 ----IWQVVFIAFLTGFLALVTIIGNILVIVAFKVNKQLKTVNNYFLLSLACADLIIGVISMNLF 124

  Fly   191 TIYVLKGYWDLGPMLCDLWLSVDYTVCLVSQYTVLLITIDRYCSVKIAAKYRSWRTRTRVIYMVT 255
            |.|::...|.||.:.||||||:||.....|...:|:|:.|||.|:.....||:.||..|...|:.
  Rat   125 TTYIIMNRWALGNLACDLWLSIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTTKRAGVMIG 189

  Fly   256 ITWIIPALLFFISIFGWEHFTGKRDLLPGQCAVQFLKDPIFNTALIIGYYWTTLIVLFVLYAGIY 320
            :.|:|..:|:..:|..|::|.|||.:.||:|.:|||.:|.......|..::..:.::.:||..||
  Rat   190 LAWVISFVLWAPAILFWQYFVGKRTVPPGECFIQFLSEPTITFGTAIAAFYMPVTIMTILYWRIY 254

  Fly   321 KTAYDMQKRSEAKQRKMQSMVALSAGAMSGMAGHAAGIGVIEEKILKTKVELAGDQTDLDSACTT 385
            |   :.:||::                                       ||||
  Rat   255 K---ETEKRTK---------------------------------------ELAG----------- 266

  Fly   386 VIKRLSGSGQANPLAVATE-EVEKM---TPEQRRASAAKIQEEAKKREAAVDAEKSERSSSPAFD 446
                |..||        || |.|..   |...|..|:.::|::..||
  Rat   267 ----LQASG--------TEAEAENFVHPTGSSRSCSSYELQQQGVKR------------------ 301

  Fly   447 SDEESSVNQAQQLITQQKLNNMRKRSSIGLVFGAQAALLATRGKGNLQKSTTNS--KSIEAMHQY 509
                               ::.||.......|                  ||.|  .|.|.|.|.
  Rat   302 -------------------SSRRKYGRCHFWF------------------TTKSWKPSAEQMDQD 329

  Fly   510 HHHQQHHHNQ---SPLQRAQSKEEMRSLHHHQNQQQPHHQHHQNQPNPPLDRPKRTSCSTLSQIA 571
            |......:|.   :.|:.:.|.:|                       ..:....|...|.:.::.
  Rat   330 HSSSDSWNNNDAAASLENSASSDE-----------------------EDIGSETRAIYSIVLKLP 371

  Fly   572 EHDRLVDLSAPPTLLNTSDPLSPVDLAPIEVPSDQVHQGLVQTILPPPDAFQCPTPLSDDYSDRP 636
            .|.         ::||:                         |.||..|..|.
  Rat   372 GHS---------SILNS-------------------------TKLPSSDNLQV------------ 390

  Fly   637 FGNSSGNSELALTYDLMTNSELRYMDESSAMLASVTANSTTSPNDSVQGKPPVLPPPPPARRNPP 701
                 .|.:|. |.|:..|:.            .:.|..:....|:.|
  Rat   391 -----SNEDLG-TVDVERNAH------------KLQAQKSMGDGDNCQ----------------- 420

  Fly   702 KSNLNQSLSQTPSQNQSPSQSLSLNLNPNHNQSQSQSIEEAVAIEKRLLVSYTGIEDFAKVRRES 766
                                                                   :||.|:
  Rat   421 -------------------------------------------------------KDFTKL---- 426

  Fly   767 CIEAICLLDVPGKLAADCPHRRASSPMETSSKDAILYSPMPPMPLSPKVKTSTPQTGTPQSAAGV 831
                                                                             
  Rat   427 ----------------------------------------------------------------- 426

  Fly   832 TPPVPLE-RIEERETSDSNTNKMPSTSGTTSSTGGVGGGGEQDGVGATKKNAGDDDANREEKSLA 895
              |:.|| .::..:|||:|::                                      .:|:.|
  Rat   427 --PIQLESAVDTGKTSDTNSS--------------------------------------ADKTTA 451

  Fly   896 ASRNSSKRAFIHSIGKHFKSKKALPLILGVGGRQKSKSENRARKAFRTISFILGCFVACWTPYHV 960
            ....|.|.|   ::.|.|..|....:.     ::|..|..:.:||.:|:|.||..|:..||||::
  Rat   452 TLPLSFKEA---TLAKRFALKTRSQIT-----KRKRMSLIKEKKAAQTLSAILLAFIITWTPYNI 508

  Fly   961 LALVEGFCRHPPCINEHLYMFSYFLCYANSPMNPFCYALANQQFKKTFTRIL 1012
            :.||..||  ..||.:..:...|:|||.||.:||.||||.|:.|:.||..:|
  Rat   509 MVLVNTFC--DSCIPKTYWNLGYWLCYINSTVNPVCYALCNKTFRTTFKTLL 558

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
mAChR-B	NP_649764.1	7tmA_mAChR_GAR-2-like	147..>323	CDD:320429	68/175 (39%)
		TM helix 2	168..194	CDD:320429	12/25 (48%)
		TM helix 3	206..236	CDD:320429	15/29 (52%)
		TM helix 4	248..271	CDD:320429	6/22 (27%)
		TM helix 5	293..322	CDD:320429	6/28 (21%)
		7tm_GPCRs	<932..1008	CDD:475119	32/75 (43%)
		TM helix 6	941..963	CDD:410628	9/21 (43%)
		TM helix 7	976..1001	CDD:410628	12/24 (50%)
Chrm3	NP_036659.2	7tmA_mAChR_M3	65..>269	CDD:320426	79/260 (30%)
		TM helix 1	69..95	CDD:320426	10/25 (40%)
		TM helix 2	102..129	CDD:320426	13/26 (50%)
		TM helix 3	140..170	CDD:320426	15/29 (52%)
		TM helix 4	182..205	CDD:320426	6/22 (27%)
		TM helix 5	227..256	CDD:320426	7/31 (23%)
		Basolateral sorting signal. /evidence=ECO:0000250	274..280		2/5 (40%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	323..356		8/55 (15%)
		7tm_GPCRs	<483..563	CDD:475119	34/78 (44%)
		TM helix 6	489..511	CDD:320426	9/21 (43%)
		TM helix 7	522..547	CDD:320426	12/24 (50%)