DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment mAChR-B and Chrm3

DIOPT Version :9

Sequence 1:	NP_649764.1	Gene:	mAChR-B / 40955	FlyBaseID:	FBgn0037546	Length:	1019	Species:	Drosophila melanogaster
Sequence 2:	NP_036659.2	Gene:	Chrm3 / 24260	RGDID:	2343	Length:	589	Species:	Rattus norvegicus

Alignment Length:	962	Identity:	192/962 - (19%)
Similarity:	303/962 - (31%)	Gaps:	441/962 - (45%)

- Green bases have known domain annotations that are detailed below.

  Fly    63 EAAKIYGESSDFNSWPVNEQLQWYRNFDVNNTNLLLAVRNQSSD--GGSTMSGSSSDSEILGPVL 125
            ||....|..:...|:.::::.   .||..|:|         |||  ||.|
  Rat    26 EAGLPLGTVTQLGSYNISQET---GNFSSNDT---------SSDPLGGHT--------------- 63

  Fly   126 PPFALWQTILIAICLAICIILTVGGNILVLLAFIVDRNIRQPSNYFIASLAATDMLIGTVSMPFY 190
                :||.:.||.......::|:.|||||::||.|::.::..:|||:.|||..|::||.:||..:
  Rat    64 ----IWQVVFIAFLTGFLALVTIIGNILVIVAFKVNKQLKTVNNYFLLSLACADLIIGVISMNLF 124

  Fly   191 TIYVLKGYWDLGPMLCDLWLSVDYTVCLVSQYTVLLITIDRYCSVKIAAKYRSWRTRTRVIYMVT 255
            |.|::...|.||.:.||||||:||.....|...:|:|:.|||.|:.....||:.||..|...|:.
  Rat   125 TTYIIMNRWALGNLACDLWLSIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTTKRAGVMIG 189

  Fly   256 ITWIIPALLFFISIFGWEHFTGKRDLLPGQCAVQFLKDPIFNTALIIGYYWTTLIVLFVLYAGIY 320
            :.|:|..:|:..:|..|::|.|||.:.||:|.:|||.:|.......|..::..:.::.:||..||
  Rat   190 LAWVISFVLWAPAILFWQYFVGKRTVPPGECFIQFLSEPTITFGTAIAAFYMPVTIMTILYWRIY 254

  Fly   321 KTAYDMQKRSEAKQRKMQSMVALSAGAMSGMAGHAAGIGVIEEKILKTKVELAGDQTDLDSACTT 385
            |   :.:||::                                       ||||
  Rat   255 K---ETEKRTK---------------------------------------ELAG----------- 266

  Fly   386 VIKRLSGSGQANPLAVATE-EVEKM---TPEQRRASAAKIQEEAKKREAAVDAEKSERSSSPAFD 446
                |..||        || |.|..   |...|..|:.::|::..||
  Rat   267 ----LQASG--------TEAEAENFVHPTGSSRSCSSYELQQQGVKR------------------ 301

  Fly   447 SDEESSVNQAQQLITQQKLNNMRKRSSIGLVFGAQAALLATRGKGNLQKSTTNS--KSIEAMHQY 509
                               ::.||.......|                  ||.|  .|.|.|.|.
  Rat   302 -------------------SSRRKYGRCHFWF------------------TTKSWKPSAEQMDQD 329

  Fly   510 HHHQQHHHNQ---SPLQRAQSKEEMRSLHHHQNQQQPHHQHHQNQPNPPLDRPKRTSCSTLSQIA 571
            |......:|.   :.|:.:.|.:|                       ..:....|...|.:.::.
  Rat   330 HSSSDSWNNNDAAASLENSASSDE-----------------------EDIGSETRAIYSIVLKLP 371

  Fly   572 EHDRLVDLSAPPTLLNTSDPLSPVDLAPIEVPSDQVHQGLVQTILPPPDAFQCPTPLSDDYSDRP 636
            .|.         ::||:                         |.||..|..|.
  Rat   372 GHS---------SILNS-------------------------TKLPSSDNLQV------------ 390

  Fly   637 FGNSSGNSELALTYDLMTNSELRYMDESSAMLASVTANSTTSPNDSVQGKPPVLPPPPPARRNPP 701
                 .|.:|. |.|:..|:.            .:.|..:....|:.|
  Rat   391 -----SNEDLG-TVDVERNAH------------KLQAQKSMGDGDNCQ----------------- 420

  Fly   702 KSNLNQSLSQTPSQNQSPSQSLSLNLNPNHNQSQSQSIEEAVAIEKRLLVSYTGIEDFAKVRRES 766
                                                                   :||.|:
  Rat   421 -------------------------------------------------------KDFTKL---- 426

  Fly   767 CIEAICLLDVPGKLAADCPHRRASSPMETSSKDAILYSPMPPMPLSPKVKTSTPQTGTPQSAAGV 831

  Rat   427 ----------------------------------------------------------------- 426

  Fly   832 TPPVPLE-RIEERETSDSNTNKMPSTSGTTSSTGGVGGGGEQDGVGATKKNAGDDDANREEKSLA 895
              |:.|| .::..:|||:|::                                      .:|:.|
  Rat   427 --PIQLESAVDTGKTSDTNSS--------------------------------------ADKTTA 451

  Fly   896 ASRNSSKRAFIHSIGKHFKSKKALPLILGVGGRQKSKSENRARKAFRTISFILGCFVACWTPYHV 960
            ....|.|.|   ::.|.|..|....:.     ::|..|..:.:||.:|:|.||..|:..||||::
  Rat   452 TLPLSFKEA---TLAKRFALKTRSQIT-----KRKRMSLIKEKKAAQTLSAILLAFIITWTPYNI 508

  Fly   961 LALVEGFCRHPPCINEHLYMFSYFLCYANSPMNPFCYALANQQFKKTFTRIL 1012
            :.||..||  ..||.:..:...|:|||.||.:||.||||.|:.|:.||..:|
  Rat   509 MVLVNTFC--DSCIPKTYWNLGYWLCYINSTVNPVCYALCNKTFRTTFKTLL 558

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
mAChR-B	NP_649764.1	7tm_1	150..>341	CDD:278431	69/190 (36%)
mAChR-B	NP_649764.1	7tm_4	150..>336	CDD:304433	69/185 (37%)
Chrm3	NP_036659.2	7tmA_mAChR_M3	65..>269	CDD:320426	79/260 (30%)
		TM helix 1	70..94	CDD:320426	10/23 (43%)
		TM helix 2	103..125	CDD:320426	11/21 (52%)
		TM helix 3	141..163	CDD:320426	10/21 (48%)
		Agonist binding. /evidence=ECO:0000305	147..151		2/3 (67%)
		TM helix 4	186..202	CDD:320426	4/15 (27%)
		TM helix 5	228..251	CDD:320426	3/22 (14%)
		Basolateral sorting signal. /evidence=ECO:0000250	274..280		2/5 (40%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	323..356		8/55 (15%)
		7tm_GPCRs	<483..563	CDD:421689	34/78 (44%)
		TM helix 6	486..513	CDD:410628	12/26 (46%)
		Agonist binding. /evidence=ECO:0000305	506..529		7/24 (29%)
		TM helix 7	522..547	CDD:410628	12/24 (50%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1245472at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.920