Protein Alignment mAChR-B and hrh1

DIOPT Version :10

• Sequence 1: NP_649764.1
• Gene: mAChR-B / 40955
• FlyBaseID: FBgn0037546
• Length: 1019
• Species: Drosophila melanogaster
• Sequence 2: XP_031757131.1
• Gene: hrh1 / 100495566
• XenbaseID: XB-GENE-6031155
• Length: 499
• Species: Xenopus tropicalis
• Alignment Length: 921
• Identity: 165/921 - (17%)
• Similarity: 241/921 - (26%)
• Gaps: 470/921 - (51%)

  Fly   130 LWQT-------------ILIAICLAICIILTVGGNILVLLAFIVDRNIRQPSNYFIASLAATDML 181
            :|||             ..:.:.|.....|||..|||||.|...:|.:....|.:|.||:..|::
 Frog     1 MWQTNQSIVQNGTEIHSASLGLALGTISALTVVLNILVLYAVRTERKLHTVGNLYIVSLSIADLI 65

  Fly   182 IGTVSMPFYTIYVLKGYWDLGPMLCDLWLSVDYTVCLVSQYTVLLITIDRYCSVKIAAKYRSWRT 246
            :|...||...:|:|...|.||...|..|||:||.....|.:::.::.||||.|::...:|..:||
 Frog    66 VGAAVMPLNIVYLLNREWILGRPACLFWLSMDYVASTASIFSLFILCIDRYRSIQQPLRYLKYRT 130

  Fly   247 RTRVIYMV------TITWIIPALLFFISIFGWEHFT--GKRDLLPGQCAVQFLKDPIFNT-ALII 302
            :||...|:      :.||::|       |.||..|.  |.|.:....|..:|.|...|.. ...:
 Frog   131 KTRASLMISGAWLLSFTWVVP-------ILGWHVFANGGIRSVPESTCETEFHKVTWFKVLTATL 188

  Fly   303 GYYWTTLIVLFVLYAGIYKTAYDMQKRSEAKQRKMQSMVALSAGAMSGMAGHAAGIGVIEEKILK 367
            .:|..:|::|: .||.|||.
 Frog   189 NFYIPSLLMLW-FYAKIYKA--------------------------------------------- 207

  Fly   368 TKVELAGDQTDLDSACTTVIKRLSGSGQANPLAVATEEVEKMTPEQRRASAAKIQEEAKKREAAV 432
                                                                 ::|..:.||.
 Frog   208 -----------------------------------------------------VREHYQHREV-- 217

  Fly   433 DAEKSERSSSPAFDSDEESSVNQAQQLITQQKLNNMRKRSSIGLVFGAQAALLATRGKGNLQKST 497
                                ||.:.||....||     ..|:.::.|.|:........|||....
 Frog   218 --------------------VNGSFQLFYDSKL-----VQSVRVLKGQQSCTKTQCKDGNLTVPE 257

  Fly   498 TNSKSIEAMHQYHHHQQHHHNQSPLQRAQSKEEMRSLHHHQNQQQPHHQHHQNQPNPPLDRPKRT 562
            .|..:     ||                                            .|:.:..|.
 Frog   258 MNFPA-----QY--------------------------------------------TPISKEFRQ 273

  Fly   563 SCSTLSQIAEHDRLVDLSAPPTLLNTSDPLSPVDLAPIEVPSDQVHQGLVQTILPPPDAFQCPTP 627
            |.||                    ...||:
 Frog   274 SRST--------------------QEKDPV----------------------------------- 283

  Fly   628 LSDDYSDRPFGNSSGNSELALTYDLMTNSELRYMDESSAMLASVTANSTTSPNDSVQGKPPVLPP 692
              :||     |...|.|               :.|.:...|...       |...:||.|
 Frog   284 --EDY-----GKQKGFS---------------HCDNNVFKLHCF-------PLAIIQGSP----- 314

  Fly   693 PPPARRNPPKSNLNQSLSQTPSQNQSPSQSLSLNLNPNHNQSQSQSIEEAVAIEKRLLVSYTGIE 757
                                                           :|.              .
 Frog   315 -----------------------------------------------QEC--------------R 318

  Fly   758 DFAKVRRESCIEAICLLDVPGKLAADCPHRRASSPMETSSKDAILYSPMPPMPLSPKVKTSTPQT 822
            ::..|.|.:..|..||.          |.:|
 Frog   319 NYVTVTRGALEEHNCLR----------PRQR---------------------------------- 339

  Fly   823 GTPQSAAGVTPPVPLERIEERETSDSNTNKMPSTSGTTSSTGGVGGGGEQDGVGATKKNAGDDDA 887
                           |.|.|..|.....:.|...|                .||.:...|    .
 Frog   340 ---------------EEISEEHTFAEAASCMVDLS----------------QVGKSNMEA----P 369

  Fly   888 NREEKSLAAS-----------RNSSKRAFIHSIGKHFKSKKALPLILGVGGRQKSKSENRARKAF 941
            .|.|..|:.:           |..||:   |..|.|.                     ||.|||.
 Frog   370 GRTEARLSGNFRYLKHTWERFRTHSKQ---HIQGLHM---------------------NRERKAA 410

  Fly   942 RTISFILGCFVACWTPYHVLALVEGFCRHPPCINEHLYMFSYFLCYANSPMNPFCYALANQQFKK 1006
            :.:.||:..|:.||.||.||.:|..||.:  |.|.:.:||:.:|.|.||.:||..|.|.|:.|||
 Frog   411 KQLGFIMAAFMLCWIPYFVLFMVIAFCHN--CCNHNFHMFTIWLGYINSTLNPLIYPLCNENFKK 473

  Fly  1007 TFTRILKGDLH 1017
            ||.:|...:|:
 Frog   474 TFKKIFHVELN 484

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
mAChR-B	NP_649764.1	7tmA_mAChR_GAR-2-like	147..>323	CDD:320429	61/184 (33%)
		TM helix 2	168..194	CDD:320429	8/25 (32%)
		TM helix 3	206..236	CDD:320429	12/29 (41%)
		TM helix 4	248..271	CDD:320429	7/28 (25%)
		TM helix 5	293..322	CDD:320429	8/29 (28%)
		7tm_GPCRs	<932..1008	CDD:475119	33/75 (44%)
		TM helix 6	941..963	CDD:410628	9/21 (43%)
		TM helix 7	976..1001	CDD:410628	10/24 (42%)
hrh1	XP_031757131.1	7tmA_Histamine_H1R	18..475	CDD:320178	158/893 (18%)
		TM helix 1	19..45	CDD:320178	10/25 (40%)
		TM helix 2	52..78	CDD:320178	8/25 (32%)
		TM helix 3	90..120	CDD:320178	12/29 (41%)
		TM helix 4	132..153	CDD:320178	6/27 (22%)
		TM helix 5	178..322	CDD:320178	46/468 (10%)
		TM helix 6	404..434	CDD:320178	14/29 (48%)
		TM helix 7	443..468	CDD:320178	10/24 (42%)