Protein Alignment SMAD4 and Mad

DIOPT Version :10

• Sequence 1: NP_005350.1
• Gene: SMAD4 / 4089
• HGNCID: 6770
• Length: 552
• Species: Homo sapiens
• Sequence 2: NP_001259992.1
• Gene: Mad / 33529
• FlyBaseID: FBgn0011648
• Length: 525
• Species: Drosophila melanogaster
• Alignment Length: 528
• Identity: 187/528 - (35%)
• Similarity: 260/528 - (49%)
• Gaps: 115/528 - (21%)

Human    20 VHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQV 84
            |..|:..:||.|.|.:|::|::|||||||::|..::.|..|::..| .|||||||.|:|||||||
  Fly    98 VKKLLGWKQGDEEEKWAEKAVDSLVKKLKKRKGAIEELERALSCPG-QPSKCVTIPRSLDGRLQV 161

Human    85 AGRKGFPHVIYARLWRWPDLHK-NELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTL 148
            :.|||.|||||.|:||||||.. :|||.::.|||.|..|...||:|||||:||.||.:....:..
  Fly   162 SHRKGLPHVIYCRVWRWPDLQSHHELKPLELCQYPFSAKQKEVCINPYHYKRVESPVLPPVLVPR 226

Human   149 QSN-APSSMMVKDEYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTA 212
            .|. ||...|::..:|    .:||:.   |::              :||....    .|::.||:
  Fly   227 HSEFAPGHSMLQFNHV----AEPSMP---HNV--------------SYSNSGF----NSHSLSTS 266

Human   213 NFPNIPVASTSQPASILGGSHSEGLLQIASGPQPGQQQNGFTGQPATYHHNSTTTWTGSRTAPYT 277
            |      .|...|:|:              ...|....:...|.|               ...|:
  Fly   267 N------TSVGSPSSV--------------NSNPNSPYDSLAGTP---------------PPAYS 296

Human   278 PNLPHHQNGHLQHHPPMPPHPGHYWPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVP 342
            |:...:.|.         |:.|... :..::.....:| :..|.:|.||||:|::.:|||.|...
  Fly   297 PSEDGNSNN---------PNDGGQL-LDAQMGDVAQVS-YSEPAFWASIAYYELNCRVGEVFHCN 350

Human   343 SSCPIVTVDGYVDPS-GGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHA 406
            ::.  |.|||:.:|| ..||.||||||||:|...||..|.||||||.|... .|:|:..||||.|
  Fly   351 NNS--VIVDGFTNPSNNSDRCCLGQLSNVNRNSTIENTRRHIGKGVHLYYV-TGEVYAECLSDSA 412

Human   407 VFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPG 471
            :||||...:...|..| ..|.||.|...:|:|:.::
  Fly   413 IFVQSRNCNYHHGFHP-STVCKIPPGCSLKIFNNQE----------------------------- 447

Human   472 PGSVGGIAPAISLSAAAGI-GVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQL 535
                  .|..:|.|...|. .|.:|.::|.:|||||||||.:|.||.:..||||||||||..||.
  Fly   448 ------FAQLLSQSVNNGFEAVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQW 506

Human   536 LDEVLHTM 543
            ||:||..|
  Fly   507 LDKVLTQM 514

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SMAD4	NP_005350.1	Mediates interaction with ZBTB7A. /evidence=ECO:0000269|PubMed:25514493	1..322		95/303 (31%)
		MH1_SMAD_4	14..138	CDD:199816	66/118 (56%)
		Required for interaction with TSC22D1. /evidence=ECO:0000269|PubMed:15881652	44..69		9/24 (38%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	168..194		3/25 (12%)
		Pneumo_att_G	<176..>281	CDD:114270	15/104 (14%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	236..256		1/19 (5%)
		SAD	275..320		6/44 (14%)
		MH2_SMAD_4	320..541	CDD:199823	91/222 (41%)
Mad	NP_001259992.1	MH1_SMAD_1_5_9	93..216	CDD:199814	66/118 (56%)
		MH2_SMAD_1_5_9	325..525	CDD:199822	93/229 (41%)