DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Sas-4 and CENPJ

DIOPT Version :9

Sequence 1:	NP_649701.2	Gene:	Sas-4 / 40859	FlyBaseID:	FBgn0011020	Length:	901	Species:	Drosophila melanogaster
Sequence 2:	NP_060921.3	Gene:	CENPJ / 55835	HGNCID:	17272	Length:	1338	Species:	Homo sapiens

Alignment Length:	1145	Identity:	275/1145 - (24%)
Similarity:	447/1145 - (39%)	Gaps:	337/1145 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 QEAGESPVGMP-LSQEAIAQRLAALSRWQDEQKRLLQERQSNHRVLLGLEQRNMYKMLGLLHQET 65
            :|....|.|.. |..|.:.:::      |:...:.||:...:..| ..:|:|.:...:|...|..
Human   281 EENRNHPTGESILCWEKVTEQI------QEANDKNLQKHDDSSEV-ANIEERPIKAAIGERKQTF 338

  Fly    66 ESHENSVLEEWD--EEHSIQMPRLAADPDEHEPEISMADPQPAKPKRPFLRRGEGLKQRFKINPD 128
            |.:    |||..  ||..::..:|    .|.|..:    |..||||:|||:|||||.
Human   339 EDY----LEEQIQLEEQELKQKQL----KEAEGPL----PIKAKPKQPFLKRGEGLA-------- 383

  Fly   129 QLRLENLPRYKFANAHPQFRTPQMKKGILKKQVKSPPNPAPPPAPKPSAQLDDLNEQRFQQ--ML 191
                      :|.||..:|:..:..| ::..|..|...|           |..::.|:.|:  .|
Human   384 ----------RFTNAKSKFQKGKESK-LVTNQSTSEDQP-----------LFKMDRQQLQRKTAL 426

  Fly   192 IGKNACSSTPDLKSSYASSTTASSTS----PRV------------------------RFVEQKSR 228
            ..|..|:..|.||....:.|.:.|.:    |::                        :|.:|...
Human   427 KNKELCADNPILKKDSKARTKSGSVTLSQKPKMLKCSNRKSLSPSGLKIQTGKKCDGQFRDQIKF 491

  Fly   229 AGQTTHADDEASSEA-SPMTGVC--WAKVLDTQ-------SIKPAQIQRRSAQIR---VEDDSNV 280
            ..:.|..:.|..:|. .|....|  |.|   ||       |..||......:.||   .|.:|::
Human   492 ENKVTSNNKENVTECPKPCDTGCTGWNK---TQGKDRLPLSTGPASRLAAKSPIRETMKESESSL 553

  Fly   281 SI----------------------FELLEQKATEGNIDMNSSCIRTFMARKD------------- 310
            .:                      |..|||.|.|.:...|||.:...:.|..
Human   554 DVSLQKKLETWEREKEKENLELDEFLFLEQAADEISFSSNSSFVLKILERDQQICKGHRMSSTPV 618

  Fly   311 ----QRRRVAD---------DSDHI---------VVTQQVR------QMRLQP-----QVDQMLV 342
                |:...||         |.||.         |..:|:.      ::|.||     .|.:...
Human   619 KAVPQKTNPADPISHCNRSEDLDHTAREKESECEVAPKQLHSLSSADELREQPCKIRKAVQKSTS 683

  Fly   343 QELQEGDEEDTE--PSSDQT--------LTMTPIQVGNTKVRVR-----------------FSDS 380
            :...|.:..|.|  |:||.:        :|:.|    :|:.|.|                 |.|:
Human   684 ENQTEWNARDDEGVPNSDSSTDSEEQLDVTIKP----STEDRERGISSREDSPQVCDDKGPFKDT 744

  Fly   381 N-------------DTHEYSDATSLNDGSNIQLFEQFKSA-------------LFQALEQ----- 414
            .             ...:||...|:.:....::.|..:|:             .:..||:
Human   745 RTQEDKRRDVDLDLSDKDYSSDESIMESIKHKVSEPSRSSSLSLSKMDFDDERTWTDLEENLCNH 809

  Fly   415 -----------------------------KKKSSP---------SKESDEPITKDLQEK------ 435
                                         |:|.:|         |:.|..|.|.:|..|
Human   810 DVVLGNESTYGTPQTCYPNNEIGILDKTIKRKIAPVKRGEDLSKSRRSRSPPTSELMMKFFPSLK 874

  Fly   436 ----------------------------ANLVRTRLEELETEIATFKEQNAQLLRLRQQHELEKA 472
                                        :.::|.::.||||||..||.:||.|.:||.:.|....
Human   875 PKPKSDSHLGNELKLNISQDQPPGDNARSQVLREKIIELETEIEKFKAENASLAKLRIERESALE 939

  Fly   473 KCTQDHMEAMERVHDEKIQAEIYLHDERMKIEEERRKFEQQMRLQKSNANSKEKKEIAALKQEVE 537
            |..::..:..::...|..:.|.:..:|..|:::||:.||:.....::..:.||::||..|||::.
Human   940 KLRKEIADFEQQKAKELARIEEFKKEEMRKLQKERKVFEKYTTAARTFPDKKEREEIQTLKQQIA 1004

  Fly   538 GLQLQLKQKEQAHVSAQARLRAQLRASEKEQRNYRDEIELLRKENKRLE----QELVKIGRENNS 598
            .|:..||:||....|..:|||:|::...:|..:.|:||:::  |..||:    .|.::...|...
Human  1005 DLREDLKRKETKWSSTHSRLRSQIQMLVRENTDLREEIKVM--ERFRLDAWKRAEAIESSLEVEK 1067

  Fly   599 KMLQEINRNIARLAPKVLPSATMSDILDENGRRTQSLDGTAGKPAKQREAQNRRQSSGSAQVRSR 663
            |  .::.....|.....:.|.|..:...:|....|.......|.|..|:..|..:...::
Human  1068 K--DKLANTSVRFQNSQISSGTQVEKYKKNYLPMQGNPPRRSKSAPPRDLGNLDKGQAAS----- 1125

  Fly   664 SRSLSRNKKTPYAL-DESYASSSSVESEEVQPPVTAPKAATPPPAANSSSDFKREITNADGSKDI 727
                .|....|... |..|....  |.:::|..::.|             |.|.|....:|.:.|
Human  1126 ----PREPLEPLNFPDPEYKEEE--EDQDIQGEISHP-------------DGKVEKVYKNGCRVI 1171

  Fly   728 WYPNGNLKKISADGMNLRMLYFNKDIKETNIREGTVKYYYAETNTWHTSYLDGLEILEFPNGQTE 792
            .:|||..|::||||..:.:.:||.|:|:. :.:..|.||||...|.||:|.:|||:|.|.:||.|
Human  1172 LFPNGTRKEVSADGKTITVTFFNGDVKQV-MPDQRVIYYYAAAQTTHTTYPEGLEVLHFSSGQIE 1235

  Fly   793 HRRKDGTVEIHFPNNSIKIVDPSDTEKLEEWRYADGTHLVQLRNGDKILNLPNGQKEIHTKLNKR 857
            ....||..||.||:.::|.:.|   :..||..:.|||.:...|:|:|::...|||:|:||...||
Human  1236 KHYPDGRKEITFPDQTVKNLFP---DGQEESIFPDGTIVRVQRDGNKLIEFNNGQRELHTAQFKR 1297

  Fly   858 REYPDGTVKLVYPDGSQETRYSNGRVRLKDKDGKLIMDTD 897
            |||||||||.||.:|.|||:|.:||:|:|||:|.::|||:
Human  1298 REYPDGTVKTVYANGHQETKYRSGRIRVKDKEGNVLMDTE 1337

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Sas-4	NP_649701.2	DUF2570	<517..>587	CDD:305162	24/73 (33%)
Sas-4	NP_649701.2	Tcp10_C	717..895	CDD:284589	79/177 (45%)
CENPJ	NP_060921.3	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	190..211
		Alpha/beta-tubulin binding. /evidence=ECO:0000269\|PubMed:19131341	319..394		31/105 (30%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	386..414		8/39 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	436..479		6/42 (14%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	521..551		7/29 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	611..789		30/181 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	845..865		4/19 (21%)
		Interaction with STIL. /evidence=ECO:0000269\|PubMed:22020124, ECO:0000269\|PubMed:25385835	895..1338		151/475 (32%)
		Ax_dynein_light	<921..1012	CDD:287215	25/90 (28%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1096..1153		11/67 (16%)
		Tcp10_C	1161..1335	CDD:284589	79/177 (45%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165159341
Domainoid	1	1.000	163	1.000	Domainoid score	I3975
eggNOG	1	0.900	-	-		E1_2CMKS
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	233	1.000	Inparanoid score	I3431
Isobase	1	0.950	-	0	Normalized mean entropy	S6950
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		oto90671
orthoMCL	1	0.900	-	-		OOG6_105142
Panther	1	1.100	-	-	LDO	PTHR10331
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R9544
SonicParanoid	1	1.000	-	-		X6202
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	13	12.730