DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CG9727 and RFX5

DIOPT Version :9

Sequence 1:	NP_001262317.1	Gene:	CG9727 / 40794	FlyBaseID:	FBgn0037445	Length:	1329	Species:	Drosophila melanogaster
Sequence 2:	NP_000440.1	Gene:	RFX5 / 5993	HGNCID:	9986	Length:	616	Species:	Homo sapiens

Alignment Length:	835	Identity:	176/835 - (21%)
Similarity:	283/835 - (33%)	Gaps:	310/835 - (37%)

- Green bases have known domain annotations that are detailed below.

  Fly   189 PATAAPSTPTIAGPRHPNEGVPAASGVINSTIAENVMNALSNSNSAGSVPSEEKMRRVLQVIETS 253
            |...:|.|    |.|.|..|..|..   .:|:.:.:...:|.:       .:.|:..:||.:: .
Human     6 PDAKSPKT----GGRAPPGGAEAGE---PTTLLQRLRGTISKA-------VQNKVEGILQDVQ-K 55

  Fly   254 LDDNAKLQISQILERVSGLKPIEKLFLYLRLPGECADTDPLRQPQNPLGTRSEINHTINWVRSHL 318
            ..||                  :||:|||:||......|...:|...  :..|..:...|:|:||
Human    56 FSDN------------------DKLYLYLQLPSGPTTGDKSSEPSTL--SNEEYMYAYRWIRNHL 100

  Fly   319 EHDAQVSIPKQDVYNDYIAYCERLS-IKPLSTADFGKVMKQVFPGVRPRRLGTRGNSRYCYAAMR 382
            |......:|||.||:.|..|||.|: .:|||||:|||:::::||.::.||||.||.|:|||:.:|
Human   101 EEHTDTCLPKQSVYDAYRKYCESLACCRPLSTANFGKIIREIFPDIKARRLGGRGQSKYCYSGIR 165

  Fly   383 KTTKLTPPQLPQLCKTEQIVAGDSNSDPSQLTSASSLGGLPSTGGDSECWDVVRSWAEKLLNTKL 447
            :.|.::.|.||.|    .:...:|.....::|.|      |..........:...|||::|....
Human   166 RKTLVSMPPLPGL----DLKGSESPEMGPEVTPA------PRDELVEAACALTCDWAERILKRSF 220

  Fly   448 ESVADLSSRI--------KSNNATV-------STKKYTPRE------------------------ 473
            .|:.:::..:        :|.:|.|       ...::.|||
Human   221 SSIVEVARFLLQQHLISARSAHAHVLKAMGLAEEDEHAPRERSSKPKNGLENPEGGAHKKPERLA 285

  Fly   474 --PKEKRVLADIGPLKKRRKKKRKGSTSSESSCNQLVTGQDAGSSQEASDEQILKIKQEIIDSPI 536
              ||:.......|||.:..:||....:|:..:.|                   |::...:...|:
Human   286 QPPKDLEARTGAGPLARGERKKSVVESSAPGANN-------------------LQVNALVARLPL 331

  Fly   537 ----HPQQ--PPIGYLQQVTPMNLATDQRSSSAVRNLT-PKILEMGSPAVVLTQQEVSPTGIVIK 594
                .|:.  |||    .|:|..|| .:.||.|::..| |.....|:|          |..:.|
Human   332 LLPRAPRSLIPPI----PVSPPILA-PRLSSGALKVATLPLSSRAGAP----------PAAVPI- 380

  Fly   595 DEVEDYTSNIFCKKVLKAQQTKGFWANSPSSGTTSGVGLLVPPTITTTSATPPPPNSETSPVPGP 659
                                                :.:::|        |.|     ..|.|||
Human   381 ------------------------------------INMILP--------TVP-----ALPGPGP 396

  Fly   660 GLSMGPPSQMMSTSSVSPSSTVDTTPKVASRNQMMILRAKRLMAAENAALAAAEDSGLPENLGLP 724
            |....||..:             |.|:                ..||..:....|.| |.:.|:.
Human   397 GPGRAPPGGL-------------TQPR----------------GTENREVGIGGDQG-PHDKGVK 431

  Fly   725 RERVISICNMD---------KHELDDYFLP-------------GEDEENS---------EEPDNE 758
            |...:.:....         |.:::|....             |..|.||         |...:.
Human   432 RTAEVPVSEASGQAPPAKAAKQDIEDTASDAKRKRGRPRKKSGGSGERNSTPLKSAAAMESAQSS 496

  Fly   759 LLQYFQMGDAEEKQLNSLDAAEQNKNPQEPPTMGSAPE-AMPAPGRGAAVVAAAAAMLAPVPAPS 822
            .|.:...|...|.  ||...||:      |..||.|.: |:.|.|:|...|
Human   497 RLPWETWGSGGEG--NSAGGAER------PGPMGEAEKGAVLAQGQGDGTV-------------- 539

  Fly   823 MLPAQAGVGSASASLALMSKKHQQQNQHQNLQLQRSKQLPLISNNKRKISLSNASSNGSNKNCIF 887
               ::.|.|..|              ||..   :...::||:.:   |:|:...|.  |.|...
Human   540 ---SKGGRGPGS--------------QHTK---EAEDKIPLVPS---KVSVIKGSR--SQKEAF- 578

  Fly   888 LPISPNTNGSVTTPTATGSSNPSQNCFASPGLTRMKQRPNLLSKQQSLDCDNRDP 942
                |...|.|.|              |..|...:|:  ::|  |.||..:::||
Human   579 ----PLAKGEVDT--------------APQGNKDLKE--HVL--QSSLSQEHKDP 611

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CG9727	NP_001262317.1	RFX_DNA_binding	307..383	CDD:280427	35/76 (46%)
RFX5	NP_000440.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..29		8/29 (28%)
		N-terminal domain	25..90		17/95 (18%)
		RFX5_N	28..86	CDD:375753	16/85 (19%)
		Leucine-rich region, critical for dimer formation and for interaction with RFXAP	62..66		2/3 (67%)
		RFX_DNA_binding	95..166	CDD:396712	35/70 (50%)
		PxLPxI/L motif, mediates interaction with RFXANK. /evidence=ECO:0000269\|PubMed:22649097	173..178		3/4 (75%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	252..314		10/61 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	391..616		64/321 (20%)
		RFX5_DNA_bdg	407..614	CDD:405326	57/292 (20%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165160487
Domainoid	1	1.000	102	1.000	Domainoid score	I6861
eggNOG	1	0.900	-	-		E1_KOG3712
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0009924
OrthoInspector	1	1.000	-	-		otm41803
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	LDO	PTHR12619
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R5454
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	8	7.920