DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CG9727 and Rfx5

DIOPT Version :9

Sequence 1:	NP_001262317.1	Gene:	CG9727 / 40794	FlyBaseID:	FBgn0037445	Length:	1329	Species:	Drosophila melanogaster
Sequence 2:	NP_001342634.1	Gene:	Rfx5 / 53970	MGIID:	1858421	Length:	658	Species:	Mus musculus

Alignment Length:	640	Identity:	143/640 - (22%)
Similarity:	241/640 - (37%)	Gaps:	158/640 - (24%)

- Green bases have known domain annotations that are detailed below.

  Fly   220 IAENVMNALSNSNSA---GSVPSEEKMRRVLQVIETSLDDNAKLQISQILERVSGLKPIEKLFLY 281
            :||:..:|.|....|   |...:.|. ..:||.:..::....:.::..||:.|......:||:||
Mouse     1 MAEDKPDAKSPKTGARPQGGADAGEP-TTLLQRLRGTISKAVQNKVEGILQEVQKFSDNDKLYLY 64

  Fly   282 LRLPGECADTDPLRQPQNPLGTRSEINHTINWVRSHLEHDAQVSIPKQDVYNDYIAYCERLS-IK 345
            |:||...:..:...:|.  |.:..|..:...|:|:|||......:|||.||:.|..|||.|: .:
Mouse    65 LQLPSGPSVGEKSSEPS--LLSNEEYMYAYRWIRNHLEEHMDTCLPKQSVYDAYRKYCESLACCR 127

  Fly   346 PLSTADFGKVMKQVFPGVRPRRLGTRGNSRYCYAAMRKTTKLTPPQLPQLCKTEQIVAGDSNSDP 410
            |||||:|||:::::||.::.||||.||.|:|||:.:|:.|.::.|.||.|     .:.|..:.:.
Mouse   128 PLSTANFGKIIREIFPDIKARRLGGRGQSKYCYSGIRRKTLVSMPPLPGL-----DLKGSESPEM 187

  Fly   411 SQLTSASSLGGLPSTGGDSECWDVVRSWAEKLLNTKLESVADLSSRI--------KSNNATV--- 464
            ....|.:....|........|     .|||::|.....|:..::..:        :|.:|.|
Mouse   188 GPEVSPAPRDELVEAACALTC-----DWAERILKRSFSSIVQVARYLLQQHLISARSAHAHVLKA 247

  Fly   465 ----STKKYTPREP---KEKRVLADI---GPLKKRRKKKRKGSTSSESSCNQLVTGQDAGSSQEA 519
                ...:..|||.   |.|.|:..:   ||    :|.:|......|....       ||:....
Mouse   248 GGLAEEDERAPRERSLCKSKNVVESLEGGGP----KKPERPAQPPKEQEAR-------AGTDLPG 301

  Fly   520 SDEQILKIKQEIIDSPI-HPQQPPIGYLQQVTPMNLATDQRSSSAVRNLTPKILEMGSPAVVLTQ 583
            ..|:    |:.:|||.: ...:|.:..|....|:.|....||.....:.|.|:..:..|..|...
Mouse   302 RAER----KKSVIDSSVPAASKPQVNALVARLPVLLPRAPRSLITPISGTLKVATLPLPTRVGGP 362

  Fly   584 QEVSPTGIVIKDEVEDYTSNIFCKKVLKAQQTKGFWANSPSSGTTSGVGLLVPPTITTTSATPPP 648
            |...|.                                         :.:::||..|.:.|.|.|
Mouse   363 QTAVPI-----------------------------------------INMILPPVPTLSGAGPGP 386

  Fly   649 ---------PNSETSPVPGPGL------SMGP-----PSQMMSTS-----SVSPSSTVDTTPKVA 688
                     |.....|.|||||      .:||     |...:...     .:.|.......| :.
Mouse   387 GPGLGPRFGPGPGLGPGPGPGLGAGLGPGLGPGLGAGPGPGLGAGLGAGLGLGPGRVPPRAP-IL 450

  Fly   689 SRNQMMILRAKRLMAAENAALAAAEDSGLPENLGLPRERVISICNMDKHELDDYFLPGEDEENSE 753
            .|            .|||..:..:.|. .|.:.|:.|...:.:                .|.:.:
Mouse   451 PR------------GAENREVGISSDP-RPHDKGIKRTAEVPL----------------SEASGQ 486

  Fly   754 EPDNELLQYFQMGDAEEKQLNSLDAAEQNKNPQEPPTMGSAPEAMPAPGRGAAVV 808
            :|..:.:::      |.:.....:|..:...|::.|  |.:.|....|.:.||:|
Mouse   487 DPPVKEMKH------ETQDTTVSEAKRKRGRPRKKP--GGSGERNATPEKSAAIV 533

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CG9727	NP_001262317.1	RFX_DNA_binding	307..383	CDD:280427	35/76 (46%)
Rfx5	NP_001342634.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..28		7/27 (26%)
		N-terminal domain. /evidence=ECO:0000250	24..89		16/67 (24%)
		RFX5_N	27..85	CDD:375753	14/59 (24%)
		Leucine-rich region, critical for dimer formation and for interaction with RFXAP. /evidence=ECO:0000250	61..65		2/3 (67%)
		RFX_DNA_binding	89..165	CDD:367002	35/75 (47%)
		PxLPxI/L motif, mediates interaction with RFXANK. /evidence=ECO:0000250\|UniProtKB:P48382	172..177		3/4 (75%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	250..315		18/79 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	382..422		12/39 (31%)
		RFX5_DNA_bdg	442..656	CDD:373167	21/130 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	443..602		21/129 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	624..658
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167850835
Domainoid	1	1.000	102	1.000	Domainoid score	I6835
eggNOG	1	0.900	-	-		E1_KOG3712
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0009924
OrthoInspector	1	1.000	-	-		otm43855
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	LDO	PTHR12619
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	7	6.930