Protein Alignment kat-60L1 and Spast

DIOPT Version :10

• Sequence 1: NP_001163523.1
• Gene: kat-60L1 / 40715
• FlyBaseID: FBgn0037375
• Length: 673
• Species: Drosophila melanogaster
• Sequence 2: NP_001156342.1
• Gene: Spast / 50850
• MGIID: 1858896
• Length: 614
• Species: Mus musculus
• Alignment Length: 654
• Identity: 190/654 - (29%)
• Similarity: 277/654 - (42%)
• Gaps: 183/654 - (27%)

  Fly   149 KKSSEGGHSSTAERHRPVNNLGSNAPGGLGIGGSVPLRSK------------------------- 188
            ||...||.|....|..|...:.:.|.|.....||.|.|:.
Mouse     9 KKKGSGGASPAPARPPPPAAVPAPAAGPAPAAGSPPKRNPSSFSSPLVVGFALLRLLACHLGLLF 73

  Fly   189 ----QRLPTQVSAAEVAPQPRASQTAQMPFPSQQQDNRWVSSLRRRDPELQPTLPSINSNANSSS 249
                ||....:.||:     |:|.||..|                    ..|:.|.......:.|
Mouse    74 AWLCQRFSRALMAAK-----RSSGTAPAP--------------------ASPSPPEPGPGGEAES 113

  Fly   250 LSQSHHGSAGNVGLAGAGAPTPAASMGTMRLGRPARASAMTAALR-KSRSVERL----------- 302
            :...|..:...:.:|             :|:....:|.....|:. ..:.:|.|
Mouse   114 VRVFHKQAFEYISIA-------------LRIDEEEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQ 165

  Fly   303 -----RARKLSTNTQLNL-----------KHKPVKKNSLDENSNSDDQDATTSLE-DNSHAQS-- 348
                 |||:|......||           |.:||.:.|   .|.:|..:.:|:|. .|.|.||
Mouse   166 GEQYERARRLQAKMMTNLVMAKDRLQLLEKLQPVLQFS---KSQTDVYNESTNLTCRNGHLQSES 227

  Fly   349 ------------------------------LATSHNTPKCS------------------------ 359
                                          |:..|..|.||
Mouse   228 GAVPKRKDPLTHASNSLPRSKTVLKSGSAGLSGHHRAPSCSGLSMVSGARPGPGPAATTHKGTPK 292

  Fly   360 ---------PKTKAKHFSPL----GYEVHLVDTLEKDILQRHPCIKWTDVAGLNEAKTILQEAVV 411
                     |.|..:....|    ..:.:|.:.:..:|:.....:|:.|:||...||..|||.|:
Mouse   293 PNRTNKPSTPTTAVRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQELAKQALQEIVI 357

  Fly   412 LPVIMPEFFKGIRRPWRGVLMVGPPGTGKTMLAKAVATECGTTFFNVSSSTLTSKYRGESEKLVR 476
            ||.:.||.|.|:|.|.||:|:.||||.||||||||||.|...||||:|:::|||||.||.|||||
Mouse   358 LPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVR 422

  Fly   477 LLFEMARFYAPSTIFIDEIDALCASRGSDSEHEASRRFKAELLIQMDGLNASMQEEKVIMVLAAT 541
            .||.:||...||.|||||:|:|...| .:.||:||||.|.|.||:.||:.::..:.  ::|:.||
Mouse   423 ALFAVARELQPSIIFIDEVDSLLCER-REGEHDASRRLKTEFLIEFDGVQSAGDDR--VLVMGAT 484

  Fly   542 NHPWDIDEAFRRRFEKRIYIPLPNEGTRSALLK--LCLKDVCLSPSLNTGI--IGDELQGYSGSD 602
            |.|.::|||..|||.||:|:.||||.||..|||  ||.:.   ||.....:  :.....||||||
Mouse   485 NRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQG---SPLTQKELAQLARMTDGYSGSD 546

  Fly   603 ISNVCRDASMMAMRRLISGRTPDQIKQIRREEVDQPITLQDFQDARLRTKKSVSADDVARFEKWM 667
            ::.:.:||::..:|.|    .|:|:|.:...|: :.|.|.||.::..:.|:|||...:..:.:|.
Mouse   547 LTALAKDAALGPIREL----KPEQVKNMSASEM-RNIRLSDFTESLKKIKRSVSPQTLEAYIRWN 606

  Fly   668 EEYG 671
            :::|
Mouse   607 KDFG 610

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
kat-60L1	NP_001163523.1	P-loop containing Nucleoside Triphosphate Hydrolases	395..561	CDD:476819	91/165 (55%)
		AAA_lid_3	591..629	CDD:465537	12/37 (32%)
		Vps4_C	<638..671	CDD:462762	9/32 (28%)
Spast	NP_001156342.1	Required for interaction with RTN1. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..298		55/329 (17%)
		Required for midbody localization. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..192		38/220 (17%)
		Required for interaction with ATL1. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..78		13/68 (19%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..48		13/38 (34%)
		Required for nuclear localization. /evidence=ECO:0000250|UniProtKB:Q9UBP0	1..48		13/38 (34%)
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021	4..11		1/1 (100%)
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000250|UniProtKB:Q9UBP0	48..85		2/36 (6%)
		Nuclear export signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021	57..65		0/7 (0%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	91..112		6/40 (15%)
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000250|UniProtKB:Q9UBP0	110..194		14/96 (15%)
		Required for interaction with microtubules. /evidence=ECO:0000250|UniProtKB:Q9UBP0	112..198		15/98 (15%)
		MIT_spastin	114..193	CDD:239142	13/91 (14%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	220..306		11/85 (13%)
		Sufficient for microtubule severing. /evidence=ECO:0000250|UniProtKB:Q9UBP0	226..614		140/396 (35%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000250|UniProtKB:Q9UBP0	268..326		5/57 (9%)
		Nuclear localization signal. /evidence=ECO:0000255|HAMAP-Rule:MF_03021	307..310		0/2 (0%)
		Required for interaction with microtubules. /evidence=ECO:0000250|UniProtKB:Q9UBP0	308..310		0/1 (0%)
		RecA-like_spastin	341..504	CDD:410932	91/165 (55%)
		AAA_lid_3	531..585	CDD:465537	18/58 (31%)
		Vps4_C	<578..610	CDD:462762	9/31 (29%)