Protein Alignment Pi4KIIalpha and Pi4k2b

DIOPT Version :9

• Sequence 1: NP_730944.2
• Gene: Pi4KIIalpha / 40670
• FlyBaseID: FBgn0037339
• Length: 710
• Species: Drosophila melanogaster
• Sequence 2: NP_080227.2
• Gene: Pi4k2b / 67073
• MGIID: 1914323
• Length: 469
• Species: Mus musculus
• Alignment Length: 568
• Identity: 275/568 - (48%)
• Similarity: 332/568 - (58%)
• Gaps: 125/568 - (22%)

  Fly   158 SIDSSSQQPPIICHIA---PAKVASSALTSPLKTTSHTALARHPHDEADDICLVPEIDFEGMSTD 219
            |.|...::.|::..:|   |.:||..            :..|...||..|:...|..|
Mouse    11 SEDEDEEREPLLPRVAWAQPRRVAPG------------SAVRMQADEGADVLREPATD------- 56

  Fly   220 AGSDNRESQPLLGGGNASHGSRISELLGGLGRGHDHVEFVS---NTFGDDPLFSEIVAKAEGAIE 281
                   ..|.:.|..:...|..:||        |.....|   |||.:||.|::||.|||.|||
Mouse    57 -------EPPAVSGEGSISASLSTEL--------DRTRTTSSETNTFLEDPEFADIVLKAEQAIE 106

  Fly   282 NGVLPERIYQGSSGSYFVKDASHQCLAVFKPKDEEPYGRLNPKWTKWMHKLCCPCCFGRACLIPN 346
            .||.||||.|||||||||||:....:.|||||.|||||:|||||||::||:||||||||.||:||
Mouse   107 IGVFPERISQGSSGSYFVKDSKRNIIGVFKPKSEEPYGQLNPKWTKYVHKVCCPCCFGRGCLLPN 171

  Fly   347 QGYLSESGASLVDRKLNLNVVPKTRVVRLVAESFNYARIDRQKAKLKKRIKEHYPSA--HFNRMS 409
            ||||||:||.|||.||||.:||||:||.||:|:|||:.|||.|::.||...|..|..  .|:|:.
Mouse   172 QGYLSEAGAYLVDVKLNLGIVPKTKVVWLVSETFNYSAIDRAKSRGKKYALEKVPKVGRKFHRIG 236

  Fly   410 LPLKTGSFQLFVEGYKDADYWLRRFESEPLPPSVAKSFQLQFERLVVLDYIIRNTDRGNDNWLIK 474
            ||.|.|||||||:.||:|:|||||||:||||.::.|.||.|||:||:||||||||||||||||:|
Mouse   237 LPPKVGSFQLFVKDYKEAEYWLRRFEAEPLPENIRKQFQSQFEKLVILDYIIRNTDRGNDNWLVK 301

  Fly   475 YVAPKITTQVGGTGGRTMHGVVTPKLPNALGASKIVDLNEPPQASPKPTTGGRPSNHLAGGAATS 539
            |...|.                         |.||
Mouse   302 YDEMKY-------------------------AKKI------------------------------ 311

  Fly   540 AQQDSSSLASSEDEPSSRSPPTSEPDWNVVNSAFIRIAAIDNGLAFPFKHPDSWRAYPYHWAWLP 604
                       |.|         |.:|.......|:||||||||||||||||.|||||:||||||
Mouse   312 -----------ESE---------ESNWIDNKQLLIKIAAIDNGLAFPFKHPDEWRAYPFHWAWLP 356

  Fly   605 QAKIPFSEEIKEQVLPQLSDMNFVEEICTELLYLFQQDRGFDKRLFERQMSVMRGQILNLTQALR 669
            |||:|||||.:..:||.:||||||:::|.:|..||:.|:|||:..||.|||||||||||||||||
Mouse   357 QAKVPFSEETRNLILPYISDMNFVQDLCEDLYELFKTDKGFDRAAFENQMSVMRGQILNLTQALR 421

  Fly   670 DGKSPVQLVQMPAVIVERS-SGSR------FFSFTQRFQNKSPFF-SW 709
            |||||:||.|||.||||.| |||:      ..||||....:.||| ||
Mouse   422 DGKSPMQLAQMPCVIVECSKSGSQGRVVHLGSSFTQTVHCRKPFFSSW 469

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pi4KIIalpha	NP_730944.2	PKc_like	293..>474	CDD:304357	125/182 (69%)
Pi4k2b	NP_080227.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..84		20/106 (19%)
		PI3_PI4_kinase	118..415	CDD:278861	198/371 (53%)
		Important for substrate binding. /evidence=ECO:0000250|UniProtKB:Q9BTU6	141..143		1/1 (100%)
		Important for interaction with membranes. /evidence=ECO:0000250|UniProtKB:Q9BTU6	149..162		9/12 (75%)
		Important for interaction with membranes. /evidence=ECO:0000250|UniProtKB:Q9BTU6	252..260		5/7 (71%)
		Important for interaction with membranes. /evidence=ECO:0000250|UniProtKB:Q9BTU6	345..354		7/8 (88%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167837150
Domainoid	1	1.000	434	1.000	Domainoid score	I571
eggNOG	1	0.900	-	-		E1_KOG2381
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	518	1.000	Inparanoid score	I1269
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002168
OrthoInspector	1	1.000	-	-		otm43079
orthoMCL	1	0.900	-	-		OOG6_101496
Panther	1	1.100	-	-	O	PTHR12865
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1894
SonicParanoid	1	1.000	-	-		X1639
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	13	12.780