DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pi4KIIalpha and pi4k2a

DIOPT Version :9

Sequence 1:	NP_730944.2	Gene:	Pi4KIIalpha / 40670	FlyBaseID:	FBgn0037339	Length:	710	Species:	Drosophila melanogaster
Sequence 2:	NP_998523.1	Gene:	pi4k2a / 406667	ZFINID:	ZDB-GENE-040426-2675	Length:	447	Species:	Danio rerio

Alignment Length:	498	Identity:	252/498 - (50%)
Similarity:	321/498 - (64%)	Gaps:	100/498 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly   232 GGGNASHGSRISELLGGLGRGHDHVEFVS------------NTFGDDPLFSEIVAKAEGAIENGV 284
            ||..::.||.:....|..||..:....:.            |.|.:||.|.||:.|||.|||.|:
Zfish    29 GGFGSTPGSVVRLPAGSPGRSRERQPLLDRDRGASPRDPHRNEFPEDPEFREIIRKAERAIEEGI 93

  Fly   285 LPERIYQGSSGSYFVKDASHQCLAVFKPKDEEPYGRLNPKWTKWMHKLCCPCCFGRACLIPNQGY 349
            .||||||||||||||||::.:.:.|||||:|||||:||||||||:.||||||||||.||:.||||
Zfish    94 YPERIYQGSSGSYFVKDSAGKIIGVFKPKNEEPYGQLNPKWTKWLQKLCCPCCFGRDCLVLNQGY 158

  Fly   350 LSESGASLVDRKLNLNVVPKTRVVRLVAESFNYARIDRQKAKLKKRIKEHYPSA--HFNRMSLPL 412
            |||:||||||:||.||:||:|:||.|.:|:|||:.|||.|::.|:...|..|..  .|:|:.||.
Zfish   159 LSEAGASLVDQKLELNIVPRTKVVYLASETFNYSAIDRVKSRGKRLALEKVPKVGQRFHRIGLPP 223

  Fly   413 KTGSFQLFVEGYKDADYWLRRFESEPLPPSVAKSFQLQFERLVVLDYIIRNTDRGNDNWLIKYVA 477
            |.||||:|||||||||:||||||:||||.:..:..||||||||||||||||||||||||||||..
Zfish   224 KVGSFQIFVEGYKDADFWLRRFEAEPLPENTNRQLQLQFERLVVLDYIIRNTDRGNDNWLIKYDY 288

  Fly   478 PKITTQVGGTGGRTMHGVVTPKLPNALGASKIVDLNEPPQASPKPTTGGRPSNHLAGGAATSAQQ 542
            |                               :|                          ||:.:
Zfish   289 P-------------------------------MD--------------------------TSSNR 296

  Fly   543 DSSSLASSEDEPSSRSPPTSEPDWNVVNSAFIRIAAIDNGLAFPFKHPDSWRAYPYHWAWLPQAK 607
            ||                    ||.:|....|::||||||||||.||||||||||::||||||||
Zfish   297 DS--------------------DWVLVKDPIIKLAAIDNGLAFPLKHPDSWRAYPFYWAWLPQAK 341

  Fly   608 IPFSEEIKEQVLPQLSDMNFVEEICTELLYLFQQDRGFDKRLFERQMSVMRGQILNLTQALRDGK 672
            :.||:||::.|||:|:|.||::::..:|..||::|.|||:..|::|:||||||||||:||:||||
Zfish   342 VVFSQEIRDLVLPKLADPNFIKDLEEDLYELFKKDPGFDRGQFKKQVSVMRGQILNLSQAMRDGK 406

  Fly   673 SPVQLVQMPAVIVE------RSSGSRFFSFTQRFQNKSPFFSW 709
            :|:||||||.||||      |::..   |:||.||::.|||:|
Zfish   407 TPLQLVQMPPVIVETARVPQRANSE---SYTQSFQSRRPFFTW 446

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pi4KIIalpha	NP_730944.2	PKc_like	293..>474	CDD:304357	126/182 (69%)
pi4k2a	NP_998523.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..77		10/47 (21%)
		PHA02777	20..>87	CDD:165142	16/57 (28%)
		PI3_PI4_kinase	102..397	CDD:278861	194/371 (52%)
		Important for substrate binding. /evidence=ECO:0000250\|UniProtKB:Q9BTU6	125..127		1/1 (100%)
		Important for interaction with membranes. /evidence=ECO:0000250\|UniProtKB:Q9BTU6	133..146		10/12 (83%)
		Important for interaction with membranes. /evidence=ECO:0000250\|UniProtKB:Q9BTU6	236..244		6/7 (86%)
		Important for interaction with membranes. /evidence=ECO:0000250\|UniProtKB:Q9BTU6	327..336		6/8 (75%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170580721
Domainoid	1	1.000	424	1.000	Domainoid score	I599
eggNOG	1	0.900	-	-		E1_KOG2381
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H101681
Inparanoid	1	1.050	517	1.000	Inparanoid score	I1248
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002168
OrthoInspector	1	1.000	-	-		otm25536
orthoMCL	1	0.900	-	-		OOG6_101496
Panther	1	1.100	-	-	LDO	PTHR12865
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1894
SonicParanoid	1	1.000	-	-		X1639
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	14	13.780