Protein Alignment Pi4KIIalpha and Pi4k2b

DIOPT Version :10

• Sequence 1: NP_730944.2
• Gene: Pi4KIIalpha / 40670
• FlyBaseID: FBgn0037339
• Length: 710
• Species: Drosophila melanogaster
• Sequence 2: NP_001005883.1
• Gene: Pi4k2b / 305419
• RGDID: 1359515
• Length: 477
• Species: Rattus norvegicus
• Alignment Length: 567
• Identity: 276/567 - (48%)
• Similarity: 331/567 - (58%)
• Gaps: 123/567 - (21%)

  Fly   158 SIDSSSQQPPIICHIAPAKVASSALTSP--LKTTSHTALARHPHDEADDICLVPEIDFEGMSTDA 220
            |.|...::.|::..:|.|:....|..|.  |:.....|:.|.|..|.      |.:..|..|..|
  Rat    19 SEDEDEEREPLLPRVAWAQPRRGAPGSAVRLQADQGAAVLREPATEE------PPVVSEDRSISA 77

  Fly   221 GSDNRESQPLLGGGNASHGSRISELLGGLGRGHDHVEFVSNTFGDDPLFSEIVAKAEGAIENGVL 285
            ..    |..|....||     :||               :|||.:||.|:::|.|||.|||.||.
  Rat    78 SL----STELDRTRNA-----VSE---------------TNTFLEDPEFADVVLKAEQAIEIGVF 118

  Fly   286 PERIYQGSSGSYFVKDASHQCLAVFKPKDEEPYGRLNPKWTKWMHKLCCPCCFGRACLIPNQGYL 350
            ||||.|||||||||||:....:.|||||.|||||:|||||||::||:||||||||.||:||||||
  Rat   119 PERISQGSSGSYFVKDSKRTIIGVFKPKSEEPYGQLNPKWTKYVHKVCCPCCFGRGCLLPNQGYL 183

  Fly   351 SESGASLVDRKLNLNVVPKTRVVRLVAESFNYARIDRQKAKLKKRIKEHYPSA--HFNRMSLPLK 413
            ||:||.|||.||.|.:||||:||.||:|:|||:.|||.|::.||...|..|..  .|:|:.||.|
  Rat   184 SEAGAYLVDTKLQLGIVPKTKVVWLVSETFNYSAIDRAKSRGKKYALEKVPKVGRKFHRIGLPPK 248

  Fly   414 TGSFQLFVEGYKDADYWLRRFESEPLPPSVAKSFQLQFERLVVLDYIIRNTDRGNDNWLIKYVAP 478
            .|||||||:.||:|:|||||||:||||.::.|.||.||||||:||||||||||||||||:||...
  Rat   249 IGSFQLFVKDYKEAEYWLRRFEAEPLPENIRKQFQSQFERLVILDYIIRNTDRGNDNWLVKYDEM 313

  Fly   479 KITTQVGGTGGRTMHGVVTPKLPNALGASKIVDLNEPPQASPKPTTGGRPSNHLAGGAATSAQQD 543
            |.                         |.||
  Rat   314 KY-------------------------AKKI---------------------------------- 319

  Fly   544 SSSLASSEDEPSSRSPPTSEPDWNVVNSAFIRIAAIDNGLAFPFKHPDSWRAYPYHWAWLPQAKI 608
                   |.|         |.:|.......||||||||||||||||||.|||||:|||||||||:
  Rat   320 -------ESE---------ESNWIDDKQLLIRIAAIDNGLAFPFKHPDEWRAYPFHWAWLPQAKV 368

  Fly   609 PFSEEIKEQVLPQLSDMNFVEEICTELLYLFQQDRGFDKRLFERQMSVMRGQILNLTQALRDGKS 673
            |||||.:..:||.:||||||:::|.:|..||:.|:|||:..||.|||||||||||||||||||||
  Rat   369 PFSEETRNLILPFISDMNFVQDLCEDLYELFKTDKGFDRAAFESQMSVMRGQILNLTQALRDGKS 433

  Fly   674 PVQLVQMPAVIVERSS----------GSRFFSFTQRFQNKSPFF-SW 709
            |:||.|||.||||.|.          ||   ||||....:.||| ||
  Rat   434 PMQLAQMPCVIVECSKSGGQGRVVHLGS---SFTQTVHCRKPFFSSW 477

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Pi4KIIalpha	NP_730944.2	Protein Kinases, catalytic domain	292..>474	CDD:473864	126/183 (69%)
Pi4k2b	NP_001005883.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..80		16/70 (23%)
		G-loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	122..128		4/5 (80%)
		PI3_PI4_kinase	125..423	CDD:395364	200/372 (54%)
		Important for substrate binding. /evidence=ECO:0000250|UniProtKB:Q9BTU6	149..151		1/1 (100%)
		Important for interaction with membranes. /evidence=ECO:0000250|UniProtKB:Q9BTU6	157..170		9/12 (75%)
		Important for interaction with membranes. /evidence=ECO:0000250|UniProtKB:Q9BTU6	260..268		5/7 (71%)
		Catalytic loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	297..305		7/7 (100%)
		Activation loop. /evidence=ECO:0000255|PROSITE-ProRule:PRU00269	338..358		18/19 (95%)
		Important for interaction with membranes. /evidence=ECO:0000250|UniProtKB:Q9BTU6	353..362		7/8 (88%)