Protein Alignment NKCC and SLC12A6

DIOPT Version :10

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: NP_598408.1
• Gene: SLC12A6 / 9990
• HGNCID: 10914
• Length: 1150
• Species: Homo sapiens
• Alignment Length: 1285
• Identity: 317/1285 - (24%)
• Similarity: 522/1285 - (40%)
• Gaps: 377/1285 - (29%)

  Fly     6 QISKVNGTANAGYDGEARSAESDQTDAAHPLPPTSNENHL-----------------------HP 47
            :|..:.|.::...|..:||:...:..:...:|.||....:                       ||
Human    21 KIDDIPGLSDTSPDLSSRSSSRVRFSSRESVPETSRSEPMSEMSGATTSLATVALDPPSDRTSHP 85

  Fly    48 K-------ASGETGEN-------RRSSRLSFRGFGNFLRKSDA--ERKFSLAQLTKESLPRLDNY 96
            :       .:..|||:       .:.:|.::....|: .:.|.  ::..:|.:...::.|::.:.
Human    86 QDVIEDLSQNSITGEHSQLLDDGHKKARNAYLNNSNY-EEGDEYFDKNLALFEEEMDTRPKVSSL 149

  Fly    97 RISMRNLKRPSIGELQGEAVDQSITIPEPEPEATGGHIKLGWIVGVLIPCLLNIWGVMLFLRLSW 161
            ...|.|....:.|..:.|.. ::||..:.:|..|.   ::|..:||.:|||.||:||:|||||:|
Human   150 LNRMANYTNLTQGAKEHEEA-ENITEGKKKPTKTP---QMGTFMGVYLPCLQNIFGVILFLRLTW 210

  Fly   162 VVAESGILQSLIIITISAVVCVITTLSLSAISTNGEVKGGGVYFIISRSLGPEFGASVGVVFAFA 226
            ||..:|:||:..|:.|.....::|.:|:|||:|||.|..||.||:|||:||||||.:||:.|...
Human   211 VVGTAGVLQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLG 275

  Fly   227 NAVSASMNTIGFCESLNVLL--------KNNDLKIVDNGINDIRIVGSITVLVLILICCVGMEWE 283
            ...:|:|..:|..|...|.:        .::.||.....:|::|:.|:..:::::|:..:|:.:.
Human   276 TTFAAAMYILGAIEIFLVYIVPRAAIFHSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYV 340

  Fly   284 TK-AQNFL--IVTIVLAIFNFLIGAAIGPQ-------GNEEQISR-------------------- 318
            .| |..||  ::..:|||:...|.::..|.       ||....||
Human   341 NKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHIDVCSKTKEINNMTVPSKL 405

  Fly   319 ----------------------------GFVGFSWATLKENFGSDYRYAEG-------------- 341
                                        |..|.:...:.||..|:| ..:|
Human   406 WGFFCNSSQFFNATCDEYFVHNNVTSIQGIPGLASGIITENLWSNY-LPKGEIIEKPSAKSSDVL 469

  Fly   342 --VNHDFFSV---------FAIFFPSVTGIQAGANICGDLKDAGAAIPKGTFWSLL----ISMSS 391
              :||::..|         ..|||||||||.||:|..||||||..:||.||..::|    :.:|:
Human   470 GSLNHEYVLVDITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSN 534

  Fly   392 YALFVLFAGGAAVRDASGIPADLVNGTIVSSELPCMATGNCTWGLFNSYEMMQEMSLWGPLIYAG 456
            ..||.....|..:||..|   |.|.|.:|        .|..:|           .|.|  :|..|
Human   535 VVLFGACIEGVVLRDKFG---DAVKGNLV--------VGTLSW-----------PSPW--VIVIG 575

  Fly   457 CFAATLSTALTNLLSVPRLVQALGIDQIYPGLIFF--SKPYGKHGEPYRGYVLTFFITTGFLLIG 519
            .|.:|....|.:|...|||:||:..|.|.|.|..|  ||   .:|||....:||..|....:||.
Human   576 SFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSK---ANGEPTWALLLTAAIAELGILIA 637

  Fly   520 ELNLIAPLISTFYLASYALINFCTFHAAFVKPLGWRPTFKYYNAWLSLFGFAMCVAIMFLINYVA 584
            .|:|:||::|.|:|..|..:|........::...|||.|:||:..||..|.::|:|:||:.::..
Human   638 SLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSWYY 702

  Fly   585 AIITFGIIFALYLVVMYRKPEANWGSTTQAQQYKAALMAVHRLQNVSDHVKNYHPQVLV---LSG 646
            ||:...|...:|..:.|:..|..||...:.....||..|:.||:....|.||:.||:||   |..
Human   703 AIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDE 767

  Fly   647 DPKTR-PPLVDFGYLLTKNNSLMFVANIIPVRVG------------YKNRQHLVKDGQKYLDARK 698
            |...: |.|:.|...|.....|..|.::|   ||            .:..:||       ::|.|
Human   768 DLHVKHPRLLTFASQLKAGKGLTIVGSVI---VGNFLENYGEALAAEQTIKHL-------MEAEK 822

  Fly   699 IKAFYNVIDGFSLEDGINALTKSTGFGKMSPNIVLVGYKPDWNRCRKEEVESYFSILYNAFSQRM 763
            :|.|..::....|.:||:.|.:|.|.|.|..|.|::|:...|   |:.|         :|.:.:.
Human   823 VKGFCQLVVAAKLREGISHLIQSCGLGGMKHNTVVMGWPNGW---RQSE---------DARAWKT 875

  Fly   764 GVALLRLPNGLDFSELSSEVTLPANGMGHMHTANAHGFTNELMPAANAASELLHIDSNLNLASMD 828
            .:..:|:                        |..||                        ||.:.
Human   876 FIGTVRV------------------------TTAAH------------------------LALLV 892

  Fly   829 SPNSSFTMPQPAPMPNMQRNSRSYKVTSSDEPAVTYHTKGGSDIPQNLLDAMTIFTRKQPKGTID 893
            :.|.||                                     .|.|:        .:..:|.||
Human   893 AKNISF-------------------------------------FPSNV--------EQFSEGNID 912

  Fly   894 VFWLYDDGGLTILLPYIISMRSHWQNSKLRVFAMCHGKDEE-QEEKSMASLLTKFRIK------- 950
            |:|:..|||:.:|||:::.....|:...:|:|.:...:|.. |.:|.:|:.|...||:
Human   913 VWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVE 977

  Fly   951 ----------YSELIMLKGVSEQPRADTVLKHKRLIEPFR-RGA-----RNEF-------GITDD 992
                      |...:|::..|:      :|:|.||.:..| |.|     ||..       ...|:
Human   978 MHDSDISAYTYERTLMMEQRSQ------MLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDE 1036

  Fly   993 ELQSMSEKT---------------------------------------NRQLRIHELVVKHSSNA 1018
            |.::..||.                                       :..::::|::|..|..|
Human  1037 ETETYQEKVHMTWTKDKYMASRGQKAKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEA 1101

  Fly  1019 SLVVMSLPMPRKEAISAPLYMSWLEMLTSDMKCPVALARGNQTPVLTLYS 1068
            .||::::|.|.:.......||.:||:||..:: .|.|.||..:.|:|:||
Human  1102 KLVLLNMPGPPRNPEGDENYMEFLEVLTEGLE-RVLLVRGGGSEVITIYS 1150

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	AA_permease_2	85..1068	CDD:459263	298/1165 (26%)
SLC12A6	NP_598408.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	20..66		8/44 (18%)
		2a30	109..1150	CDD:273347	302/1195 (25%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	161..181		5/20 (25%)
		Scissor helix. /evidence=ECO:0000269|PubMed:33199848, ECO:0000269|PubMed:34031912, ECO:0007744|PDB:6M1Y, ECO:0007744|PDB:6Y5R, ECO:0007744|PDB:6Y5V, ECO:0007744|PDB:7AIN, ECO:0007744|PDB:7AIO, ECO:0007744|PDB:7D90, ECO:0007744|PDB:7NGB	682..691		3/8 (38%)
		Interaction with CKB. /evidence=ECO:0000269|PubMed:18566107	1133..1150		6/17 (35%)