Protein Alignment NKCC and Slc12a2

DIOPT Version :9

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: NP_113986.2
• Gene: Slc12a2 / 83629
• RGDID: 620809
• Length: 1203
• Species: Rattus norvegicus
• Alignment Length: 1138
• Identity: 456/1138 - (40%)
• Similarity: 665/1138 - (58%)
• Gaps: 138/1138 - (12%)

  Fly     4 RFQISKVNGTANAGYDGEARSAESDQTDAA---HPLPPTSNENHLHPKASGETGENRRSSRLSFR 65
            ||:::.|:..|::       ||:...:|||   ...|..|.:|      .|:|..:..||..|..
  Rat   131 RFRVNFVDPAASS-------SADDSLSDAAGVGGDGPNVSFQN------GGDTVLSEGSSLHSGG 182

  Fly    66 GFGN-------------FLRKSDAERKFSLAQLTKESLPRLDNYRISM----RNLKRPSIGELQG 113
            |.|:             :||        :....|.:::||:|:||.:.    ..|.|||:.||..
  Rat   183 GSGHHQQYYYDTHTNTYYLR--------TFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHD 239

  Fly   114 EAVDQSITIPEPEPEATG----------------------GHIKLGWIVGVLIPCLLNIWGVMLF 156
            |.        |.||...|                      |.:|.|||.|||:.|:|||||||||
  Rat   240 EL--------EKEPFEDGFANGEESTPTRDAVVTYTAESKGVVKFGWIKGVLVRCMLNIWGVMLF 296

  Fly   157 LRLSWVVAESGILQSLIIITISAVVCVITTLSLSAISTNGEVKGGGVYFIISRSLGPEFGASVGV 221
            :||||:|.::||..|:::|.::.||..||.||.|||:|||.|:|||.|::||||||||||.::|:
  Rat   297 IRLSWIVGQAGIGLSVLVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGL 361

  Fly   222 VFAFANAVSASMNTIGFCESLNVLLKNNDLKIVDNGINDIRIVGSITVLVLILICCVGMEWETKA 286
            :|||||||:.:|..:||.|::..|||.:.:.::|. ||||||:|:|||:||:.|...|||||.||
  Rat   362 IFAFANAVAVAMYVVGFAETVVELLKEHSILMIDE-INDIRIIGAITVVVLLGISVAGMEWEAKA 425

  Fly   287 QNFLIVTIVLAIFNFLIGAAIGPQGNEEQISRGFVGFSWATLKENFGSDYRYAEGVNHDFFSVFA 351
            |..|:|.::|||.:|:||..|.....:   .:||.|:......||||.|:|..|    .||||||
  Rat   426 QIVLLVILLLAIADFVIGTFISLDSKK---PKGFFGYKSEIFSENFGPDFREEE----TFFSVFA 483

  Fly   352 IFFPSVTGIQAGANICGDLKDAGAAIPKGTFWSLLISMSSYALFVLFAGGAAVRDASG-----IP 411
            ||||:.|||.|||||.|||.|..:||||||..::||:...|....:..|...||||:|     |.
  Rat   484 IFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTVVYIGIAVSVGSCVVRDATGNVNDTIT 548

  Fly   412 ADLVNGTIVSSELP-----CMATGNCTWGLFNSYEMMQEMSLWGPLIYAGCFAATLSTALTNLLS 471
            .:|.|.|..:.:|.     | .:..|::||.|::::|..:|.:.|||.||.|:||||:||.:|:|
  Rat   549 TELTNCTSAACKLNFDFSYC-ESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVS 612

  Fly   472 VPRLVQALGIDQIYPGLIFFSKPYGKHGEPYRGYVLTFFITTGFLLIGELNLIAPLISTFYLASY 536
            .|::.|||..|.|||....|:|.|||:.||.|||:|||.|..||:||.|||:|||:||.|:||||
  Rat   613 APKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASY 677

  Fly   537 ALINFCTFHAAFVKPLGWRPTFKYYNAWLSLFGFAMCVAIMFLINYVAAIITFGIIFALYLVVMY 601
            |||||..|||:..|..||||.|||||.|:||.|..:|..:||:||:.||::|:.|:..||:.|.|
  Rat   678 ALINFSVFHASLAKSPGWRPAFKYYNMWISLTGAILCCIVMFVINWWAALLTYVIVLGLYIYVTY 742

  Fly   602 RKPEANWGSTTQAQQYKAALMAVHRLQNVSDHVKNYHPQVLVLSGDPKTRPPLVDFGYLLTKNNS 666
            :||:.||||:|||..|.:||....||..|.|||||:.||.||::|.|.:||.|:...:..|||..
  Rat   743 KKPDVNWGSSTQALTYLSALQHSIRLSGVEDHVKNFRPQCLVMTGSPNSRPALLHLVHDFTKNVG 807

  Fly   667 LMFVANII--PVRVGYKNRQHLVKDGQKYLDARKIKAFYNVIDGFSLEDGINALTKSTGFGKMSP 729
            ||...::.  |.|...|.........|::|...|:||||..:....|.:|...|.::.|.|:|.|
  Rat   808 LMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKP 872

  Fly   730 NIVLVGYKPDWNRCRKEEVESYFSILYNAFSQRMGVALLRLPNGLDFSELSSEVTLPANGMGHMH 794
            |.:::|:|.||.:....:|:.|.::.::||..:.||.::||..|||.|.|..:
  Rat   873 NTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQGQ------------ 925

  Fly   795 TANAHGFTNELMPAANAASELLHIDSNLNLASMDSPNSSFTMPQPAPMPNMQRNSRSYKVTSSDE 859
                    .||:.:...:.....:..|::. |..|...:|......|:  .|::......||: :
  Rat   926 --------EELLSSQEKSPGTKDVVVNVDY-SKKSDQDAFKASGEKPI--TQKDEEEDGKTST-Q 978

  Fly   860 PAVTYHTKGG----SDIPQNLLDAMTIFTRKQPKGTIDVFWLYDDGGLTILLPYIISMRSHWQNS 920
            |.:...:||.    :...|.||:|.|.|.:||.|.||||:||:||||||:|:||:::.:..|::.
  Rat   979 PLLKKESKGPVAPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDC 1043

  Fly   921 KLRVFAMCHGKDE--EQEEKSMASLLTKFRIKYSELIMLKGVSEQPRADTVLKHKRLIEPFRRGA 983
            |:|||  ..||..  :.:.::||:||:||||.:|::::|..::.:|:.:.::....:|||:|...
  Rat  1044 KIRVF--IGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIVAFDDMIEPYRLHE 1106

  Fly   984 RNE-------------FGITDDELQSMSEKTNRQLRIHELVVKHSSNASLVVMSLPMPRKEAISA 1035
            .::             :.|||:||:....||.||:|::||:.:|||.|:::|||||:.||.|:|:
  Rat  1107 DDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSS 1171

  Fly  1036 PLYMSWLEMLTSDMKCPVALARGNQTPVLTLYS 1068
            .|||:|||.|:.|:. |:.|.|||...|||.||
  Rat  1172 ALYMAWLEALSKDLP-PILLVRGNHQSVLTFYS 1203

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	2a30	85..1068	CDD:273347	433/1039 (42%)
		DUF502	507..>643	CDD:294696	78/135 (58%)
		SLC12	651..1068	CDD:281515	143/437 (33%)
Slc12a2	NP_113986.2	2a30	207..1203	CDD:273347	433/1039 (42%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG2083
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG57166
OrthoDB	1	1.010	-	-		D135130at33208
OrthoFinder	1	1.000	-	-		FOG0001536
OrthoInspector	1	1.000	-	-		mtm8955
orthoMCL	1	0.900	-	-		OOG6_101360
Panther	1	1.100	-	-	O	PTHR11827
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	11	10.790