Protein Alignment NKCC and slc12a5b

DIOPT Version :10

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: XP_009302423.1
• Gene: slc12a5b / 797331
• ZFINID: ZDB-GENE-080707-1
• Length: 1126
• Species: Danio rerio
• Alignment Length: 1284
• Identity: 303/1284 - (23%)
• Similarity: 484/1284 - (37%)
• Gaps: 403/1284 - (31%)

  Fly    21 EARSAESDQTDAAHPLPPTSNENHLHPKASGETGENRRSSRLSFRGFGNFLRKSDAERKFSLAQL 85
            |.....:.|.||      ||.|:  .|..:..|.:..:|.:...:....|      |.:...:.:
Zfish    10 EGDGGPNSQGDA------TSKES--SPFINSSTSDVEKSQQYDGKNMALF------EEEMDTSPM 60

  Fly    86 TKESLPRLDNYRISMRNLKRPSIGELQGEAVDQSITIPEPEPEATGGHIKLGWIVGVLIPCLLNI 150
            ....|..|.||....:..|.....|...||..::...|           ::|.|:||.:|||.||
Zfish    61 VSSLLSSLANYSNLTQGSKEHEEAENNEEARKKAAQAP-----------RMGTIMGVYLPCLQNI 114

  Fly   151 WGVMLFLRLSWVVAESGILQSLIIITISAVVCVITTLSLSAISTNGEVKGGGVYFIISRSLGPEF 215
            .||:||||::|:|...|:|.:..|:.:.....::|.:|:|||:|||.|..||.|::|||||||||
Zfish   115 LGVILFLRMTWLVGVGGVLGTFTIVFMCCSTTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEF 179

  Fly   216 GASVGVVFAFANAVSASMNTIGFCESLNVLLKNNDLKIVDNG----------INDIRIVGSITVL 270
            |.:||:.|......:.:|..:|..|.|.:.:..:.......|          :|::|:.|:|.:.
Zfish   180 GGAVGICFFLGTTFAGAMYILGCIEILLIYIVPSAAIFKMEGLEGSEAEAALLNNMRVYGTIVLT 244

  Fly   271 VLILICCVGMEWETK-AQNFL--IVTIVLAIFNFLIGAAIGPQ-------GNEEQISR------- 318
            .:.::..||:::..| |..||  ::..:|||:..:|..:..|.       ||...:|:
Zfish   245 FMAIVVFVGVKYVNKLALVFLACVILSILAIYAGVIKTSFDPPDFPVCVLGNRTLVSKAYDICAK 309

  Fly   319 ----------------------------------------GFVGFSWATLKENFGSDY------- 336
                                                    |..|.:...|.||..|.|
Zfish   310 TIERGNATITTKLWRSFCDSEFLNATCDEYFVNNNISQIQGIPGVTSGILAENLFSGYMEKNSVL 374

  Fly   337 -------------------RYAEGVNHDFFSVF-AIFFPSVTGIQAGANICGDLKDAGAAIPKGT 381
                               ||.......||::. .|:|||||||.||:|..|||:||..:||.||
Zfish   375 EKRGLQAVQDPELPVTNSNRYVLADITSFFTLLVGIYFPSVTGIMAGSNRSGDLQDAQKSIPVGT 439

  Fly   382 FWSL----LISMSSYALFVLFAGGAAVRD--ASGIPADLVNGTIVSSELPCMATGNCTWGLFNSY 440
            ..::    :|.|||..||.....|..:||  ..|:..:||.||:             .|
Zfish   440 ILAITTTSIIYMSSVILFGACVDGVVLRDKFGEGVSGNLVIGTL-------------AW------ 485

  Fly   441 EMMQEMSLWGPLIYAGCFAATLSTALTNLLSVPRLVQALGIDQIYPGLIFFSKPYGK-HGEPYRG 504
                 .|.|  :|..|.|.:|....|.:|...|||:||:..|.|.|.|..|.  :|| :|||...
Zfish   486 -----PSPW--VIVFGSFFSTCGAGLQSLTGAPRLLQAIARDGIIPFLRVFG--HGKANGEPTWA 541

  Fly   505 YVLTFFITTGFLLIGELNLIAPLISTFYLASYALINFCTFHAAFVKPLGWRPTFKYYNAWLSLFG 569
            .:||..|....:||..|:.:||::|.|:|..|..:|........::...|||.||:|:..|||.|
Zfish   542 LLLTACICESGILIASLDAVAPILSMFFLMCYMFVNLACALQTLLRTPNWRPRFKFYHWALSLLG 606

  Fly   570 FAMCVAIMFLINYVAAIITFGIIFALYLVVMYRKPEANWGSTTQAQQYKAALMAVHRLQNVSDHV 634
            .::|:.:|||.::..||:...|...:|..:.:...|..||...:.....||..|:.||:....|.
Zfish   607 MSLCLTLMFLCSWYYAIVAMVIAGCIYKYIEFCGAEKEWGDGIRGISLSAARFALMRLEEGPPHT 671

  Fly   635 KNYHPQVLVLS---GDPKT-RPPLVDFGYLLTKNNSLMFVANIIPVRVGYKNRQHLVKDG----Q 691
            ||:.||:|||:   |:... :|.|:.....|.....|..|...|  ...|.|.|...:..    :
Zfish   672 KNWRPQILVLTTLDGEQNVEQPRLLSLTSQLKAGKGLTIVGACI--EGTYLNNQPKTQKADQSLR 734

  Fly   692 KYLDARKIKAFYNVIDGFSLEDGINALTKSTGFGKMSPNIVLVGYKPDW------NRCRKEEVES 750
            |.::..|:|.|..|:...:|.|..:.|.::.|.|.:..|.|||.:..:|      :|||
Zfish   735 KLMEVEKVKGFSQVVISSNLRDATSHLIQAGGLGGLRHNTVLVSFPKNWKQAEEHHRCR------ 793

  Fly   751 YFSILYNAFSQRMGVALLRLPNGLDFSELSSEVTLPANGMGHMHTANAHGFTNELMPAANAASEL 815
                                    :|.|:..|.|     .|||..         |:|.
Zfish   794 ------------------------NFIEVVRETT-----AGHMAL---------LVPK------- 813

  Fly   816 LHIDSNLNLASMDSPNSSFTMPQPAPMPNMQRNSRSYKVTSSDEPAVTYHTKGGSDIPQNLLDAM 880
                   |:::..|....||
Zfish   814 -------NISAYPSNGERFT--------------------------------------------- 826

  Fly   881 TIFTRKQPKGTIDVFWLYDDGGLTILLPYIISMRSHWQNSKLRVFAMCHGKDEE-QEEKSMASLL 944
                    :|.|||:|:..|||:.:|||:::.....|:..|:|:|.:....|.. |.:|.:.:.|
Zfish   827 --------EGHIDVWWIVHDGGMLMLLPFLLRQHKVWRKCKMRIFTVAQMDDNSIQMKKDLMTFL 883

  Fly   945 TKFRI-----------------KYSELIMLKGVS------------------------------- 961
            ...|:                 .|.:.:|::..|
Zfish   884 YHLRLDAAVEVVEMLDNDISAYTYEKTLMMEQRSQILKEMHLTKNEREREIQSITDVSRGSIRRK 948

  Fly   962 ---------------------EQPRADTV-LKHKRLIE-----------------------PFRR 981
                                 |:|..::| :.|.|.::                       ....
Zfish   949 NPSNLHPQRSIAEESVAGSGEEKPEEESVRVSHPRQVQLIQGKNATPTPTSPTSPTAPTPATMSP 1013

  Fly   982 GARNEFGITDD-----------------------ELQSMSEKTNRQL----RIHELVVKHSSNAS 1019
            |...:...|||                       |.:::::...|::    :::|::||.|..|.
Zfish  1014 GEEIQMTWTDDTEKANNPAVANPENIKDMFNMKPEWENLNQSNVRRMHHAQKLNEVIVKKSQEAK 1078

  Fly  1020 LVVMSLPMPRKEAISAPLYMSWLEMLTSDMKCPVALARGNQTPVLTLYS 1068
            ||::::|.|.:.......||.:||:||..:. .|.|.||....|:|:||
Zfish  1079 LVLLNMPGPPRNRTGEENYMEFLEVLTEGLN-RVLLVRGGGREVITIYS 1126

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	AA_permease_2	85..1068	CDD:459263	289/1218 (24%)
slc12a5b	XP_009302423.1	AA_permease_2	24..1126	CDD:459263	295/1262 (23%)