Protein Alignment NKCC and SLC12A4

DIOPT Version :10

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: NP_001139434.1
• Gene: SLC12A4 / 6560
• HGNCID: 10913
• Length: 1087
• Species: Homo sapiens
• Alignment Length: 1145
• Identity: 284/1145 - (24%)
• Similarity: 457/1145 - (39%)
• Gaps: 316/1145 - (27%)

  Fly   105 RPSIGELQGEAVD-QSITIPEPE-PEATGGH---------IKLGWIVGVLIPCLLNIWGVMLFLR 158
            ||.:..|.|:.|. .::|....| .||..|.         ..:|.::||.:|||.||:||:||||
Human    78 RPKVSSLLGKLVSYTNLTQGAKEHEEAESGEGTRRRAAEAPSMGTLMGVYLPCLQNIFGVILFLR 142

  Fly   159 LSWVVAESGILQSLIIITISAVVCVITTLSLSAISTNGEVKGGGVYFIISRSLGPEFGASVGVVF 223
            |:|:|..:|:||:|:|:.|.....::|.:|:|||:|||.|..||.||:||||||||||.:||:.|
Human   143 LTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCF 207

  Fly   224 AFANAVSASMNTIGFCESLNVLLKNNDLKIVDNG--------INDIRIVGSITVLVLILICCVGM 280
            ......:|:|..:|..|.|...:.........:|        :|::|:.|:|.:..:.|:..||:
Human   208 YLGTTFAAAMYILGAIEILLTYIAPPAAIFYPSGAHDTSNATLNNMRVYGTIFLTFMTLVVFVGV 272

  Fly   281 EWETKAQNFLIVTIVLAIFNFLIG---AAIGPQ-------GNEEQISR----------------- 318
            ::..|..:..:..::::|.:...|   :...|.       || ..:||
Human   273 KYVNKFASLFLACVIISILSIYAGGIKSIFDPPVFPVCMLGN-RTLSRDQFDICAKTAVVDNETV 336

  Fly   319 --------------------------------GFVGFSWATLKENFGSDY--------------- 336
                                            |..|.:...|:||..|.|
Human   337 ATQLWSFFCHSPNLTTDSCDPYFMLNNVTEIPGIPGAAAGVLQENLWSAYLEKGDIVEKHGLPSA 401

  Fly   337 -----------RYAEGVNHDFFSVFAIFFPSVTGIQAGANICGDLKDAGAAIPKGTFWSL----L 386
                       .....:...|..:..|||||||||.||:|..|||:||..:||.||..::    |
Human   402 DAPSLKESLPLYVVADIATSFTVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIITTSL 466

  Fly   387 ISMSSYALFVLFAGGAAVRD--ASGIPADLVNGTIVSSELPCMATGNCTWGLFNSYEMMQEMSLW 449
            :..||..||.....|..:||  ..|:..:||.||:             .|           .|.|
Human   467 VYFSSVVLFGACIEGVVLRDKYGDGVSRNLVVGTL-------------AW-----------PSPW 507

  Fly   450 GPLIYAGCFAATLSTALTNLLSVPRLVQALGIDQIYPGLIFFSKPYGK-HGEPYRGYVLTFFITT 513
              :|..|.|.:|....|.:|...|||:||:..|.|.|.|..|.  :|| :|||....:||..|..
Human   508 --VIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFG--HGKVNGEPTWALLLTALIAE 568

  Fly   514 GFLLIGELNLIAPLISTFYLASYALINFCTFHAAFVKPLGWRPTFKYYNAWLSLFGFAMCVAIMF 578
            ..:||..|:::||::|.|:|..|..:|........::...|||.||||:..||..|.::|:|:||
Human   569 LGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWALSFLGMSLCLALMF 633

  Fly   579 LINYVAAIITFGIIFALYLVVMYRKPEANWGSTTQAQQYKAALMAVHRLQNVSDHVKNYHPQVLV 643
            :.::..|::...|...:|..:.|:..|..||...:.....||..|:.||:....|.||:.||:||
Human   634 VSSWYYALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLV 698

  Fly   644 ---LSGDPKTR-PPLVDFGYLLTKNNSLMFVANII--PVRVGYKNRQHLVKDGQKYLDARKIKAF 702
               |..|...: |.|:.|...|.....|..|.::|  .....|...|...:..:..::..|:|.|
Human   699 LLKLDEDLHVKYPRLLTFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMEIEKVKGF 763

  Fly   703 YNVIDGFSLEDGINALTKSTGFGKMSPNIVLVGYKPDWNRCRKEEVESYFSILYNAFSQRMGVAL 767
            ..|:....:.:|:..|.:|.|.|.|..|.|::|:...|.:.........|            :..
Human   764 CQVVVASKVREGLAHLIQSCGLGGMRHNSVVLGWPYGWRQSEDPRAWKTF------------IDT 816

  Fly   768 LRLPNGLDFSELSSEVTLPANGMGHMHTANAHGFTNELMPAANAASELLHIDSNLNLASMDSPNS 832
            :|.                        |..||                        ||.:...|.
Human   817 VRC------------------------TTAAH------------------------LALLVPKNI 833

  Fly   833 SFTMPQPAPMPNMQRNSRSYKVTSSDEPAVTYHTKGGSDIPQNLLDAMTIFTRKQPKGTIDVFWL 897
            :|                                     .|.|        ..:..:|.|||:|:
Human   834 AF-------------------------------------YPSN--------HERYLEGHIDVWWI 853

  Fly   898 YDDGGLTILLPYIISMRSHWQNSKLRVFAMCHGKDEE-QEEKSMASLLTKFRIK----------- 950
            ..|||:.:|||:::.....|:..::|:|.:....|.. |.:|.:|..|...|::
Human   854 VHDGGMLMLLPFLLRQHKVWRKCRMRIFTVAQMDDNSIQMKKDLAVFLYHLRLEAEVEVVEMHNS 918

  Fly   951 ------YSELIMLKGVSEQPRADTVLKHKR-----LIEPFRRGARNEFGITDDELQS-------- 996
                  |...:|::..|:..|...:.|.:|     |::......|.|...:|:|.:|
Human   919 DISAYTYERTLMMEQRSQMLRQMRLTKTEREREAQLVKDRHSALRLESLYSDEEDESAVGADKIQ 983

  Fly   997 --------MSE-------------------------KTNRQLRIHELVVKHSSNASLVVMSLPMP 1028
                    |:|                         :.:..::::|::|..|.:|.||::::|.|
Human   984 MTWTRDKYMTETWDPSHAPDNFRELVHIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGP 1048

  Fly  1029 RKEAISAPLYMSWLEMLTSDMKCPVALARGNQTPVLTLYS 1068
            .:.:.....||.:||:||..:: .|.|.||....|:|:||
Human  1049 PRNSEGDENYMEFLEVLTEGLE-RVLLVRGGGREVITIYS 1087

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	AA_permease_2	85..1068	CDD:459263	282/1143 (25%)
SLC12A4	NP_001139434.1	2a30	70..1087	CDD:273347	282/1143 (25%)