Protein Alignment NKCC and SLC12A3

DIOPT Version :9

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: NP_000330.3
• Gene: SLC12A3 / 6559
• HGNCID: 10912
• Length: 1030
• Species: Homo sapiens
• Alignment Length: 1122
• Identity: 436/1122 - (38%)
• Similarity: 620/1122 - (55%)
• Gaps: 170/1122 - (15%)

  Fly     4 RFQISKVNGTANAGYDGEARSAESDQTDAAHPLPP----TSNENHL------------------- 45
            ||.||.:              ..||:     |.||    :|:.:||
Human    19 RFTISTL--------------LSSDE-----PSPPAAYDSSHPSHLTHSSTFCMRTFGYNTIDVV 64

  Fly    46 ----HPKASGETGENRRSSRLSFRGFGNFLRKSDAERKFSLAQLTKESLPRLDNYRISMRNLKRP 106
                |...|.:.||.|: .|.:.....:|| |.:.....:||..::.|....|
Human    65 PTYEHYANSTQPGEPRK-VRPTLADLHSFL-KQEGRHLHALAFDSRPSHEMTD------------ 115

  Fly   107 SIGELQGEAVDQSITIPEPEPEATGGHIKLGWIVGVLIPCLLNIWGVMLFLRLSWVVAESGILQS 171
              |.::|||...|...|| ||      ::.||:.||:|.|:||||||:|:|||.|:.|::||:.:
Human   116 --GLVEGEAGTSSEKNPE-EP------VRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLT 171

  Fly   172 LIIITISAVVCVITTLSLSAISTNGEVKGGGVYFIISRSLGPEFGASVGVVFAFANAVSASMNTI 236
            .|||.:|..|..||.||:|||||||:||.||.||:||||||||.|.|:|::|||||||..:|:|:
Human   172 WIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTV 236

  Fly   237 GFCESLNVLLKNNDLKIVDNGINDIRIVGSITVLVLILICCVGMEWETKAQNFLIVTIVLAIFNF 301
            ||.|::..||:.....||| .||||||:..::|.||:.|...|||||:|||....:.|:::..|:
Human   237 GFAETVRDLLQEYGAPIVD-PINDIRIIAVVSVTVLLAISLAGMEWESKAQVLFFLVIMVSFANY 300

  Fly   302 LIGAAIGPQGNEEQISRGFVGFSWATLKENFGSDYRYAEGVNHDFFSVFAIFFPSVTGIQAGANI 366
            |:|..|.|  :|::.|:||..:......:|...|:|..:|.   ||.:|:|||||.|||.|||||
Human   301 LVGTLIPP--SEDKASKGFFSYRADIFVQNLVPDWRGPDGT---FFGMFSIFFPSATGILAGANI 360

  Fly   367 CGDLKDAGAAIPKGTFWSLLISMSSYALFVLFAGGAAVRDASGIPADLV-------NGTIVS--- 421
            .|||||...||||||..::..:..||.......|...||||||:..|.|       .|...|
Human   361 SGDLKDPAIAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGVLNDTVTPGWGACEGLACSYGW 425

  Fly   422 SELPCMATGNCTWGLFNSYEMMQEMSLWGPLIYAGCFAATLSTALTNLLSVPRLVQALGIDQIYP 486
            :...|....:|.:||.|.|:.|..:|.:.|||.||.|.||||:||..|:|..::.|.|..||:||
Human   426 NFTECTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYP 490

  Fly   487 GLIFFSKPYGKHGEPYRGYVLTFFITTGFLLIGELNLIAPLISTFYLASYALINFCTFHAAFVKP 551
            .:.||.|.|||:.||.|||:|.:.|...|::|.|||.|||:||.|:|.|||||||..|||:....
Human   491 LIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNS 555

  Fly   552 LGWRPTFKYYNAWLSLFGFAMCVAIMFLINYVAAIITFGIIFALYLVVMYRKPEANWGSTTQAQQ 616
            .||||:|:|||.|.:|||..:.|.||||:.:.||:|..|::..|.|.|:|:|||.||||:.||..
Human   556 PGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGS 620

  Fly   617 YKAALMAVHRLQNVSDHVKNYHPQVLVLSGDPKTRPPLVDFGYLLTKNNSLMFVANIIPVRVG-Y 680
            |..||.....|..|.||:|||.||.|||:|.|..||.||||....|:|.|||...:::   :| :
Human   621 YNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVL---IGPH 682

  Fly   681 KNRQ---HLVKDGQ-KYLDARKIKAFYNVIDGFSLEDGINALTKSTGFGKMSPNIVLVGYKPDWN 741
            |.|.   .|:.:|. |:|:.|||||||:.:....|..|:..|.::.|.|:|.|||::||:|.:|.
Human   683 KQRMPELQLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQ 747

  Fly   742 RCRKEEVESYFSILYNAFSQRMGVALLRLPNGLDFSELSSEVTLPANGMGHMHTANAHGFTNELM 806
            ......||.|..||::||....||.::|:..||:.|::                ..||     :.
Human   748 SAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKM----------------MQAH-----IN 791

  Fly   807 PAANAASELLHIDSNLNLASMDSPNSSFTMPQPAPMPNMQRNSRSYKVTSSDEPAVTYHTKGGSD 871
            |..:.|.              |...:|            .|.:|         |:|:    |..|
Human   792 PVFDPAE--------------DGKEAS------------ARGAR---------PSVS----GALD 817

  Fly   872 IPQNLL---DAMTIFTRKQPKGTIDVFWLYDDGGLTILLPYIISMRSHWQNSKLRVFAMCHGKDE 933
             |:.|:   .|.|||..:|.|.|||::||:||||||:|:||::..:..|...|:|||........
Human   818 -PKALVKEEQATTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRVFVGGQINRM 881

  Fly   934 EQEEKSMASLLTKFRIKYSELIMLKGVSEQPRADTVLKHKRLIEPFR--RGARNE---------- 986
            :||.|::.|||:|||:.:.|:.:|..:::.|||:...:.:.:|.|||  .|.::|
Human   882 DQERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDC 946

  Fly   987 -FGITDDELQSMSEKTNRQLRIHELVVKHSSNASLVVMSLPMPRKEAISAPLYMSWLEMLTSDMK 1050
             :.|:|:|:.....|:.||:|::|:|:.:|.:|:|:|::||:.||....:.|||:|||.|:.|::
Human   947 PWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYMAWLETLSQDLR 1011

  Fly  1051 CPVALARGNQTPVLTLY 1067
            .||.|.||||..|||.|
Human  1012 PPVILIRGNQENVLTFY 1028

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	2a30	85..1068	CDD:273347	413/1014 (41%)
		DUF502	507..>643	CDD:294696	70/135 (52%)
		SLC12	651..1068	CDD:281515	146/438 (33%)
SLC12A3	NP_000330.3	2a30	59..1029	CDD:273347	424/1063 (40%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG2083
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG57166
OrthoDB	1	1.010	-	-		D135130at33208
OrthoFinder	1	1.000	-	-		FOG0001536
OrthoInspector	1	1.000	-	-		mtm8484
orthoMCL	1	0.900	-	-		OOG6_101360
Panther	1	1.100	-	-	O	PTHR11827
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	10	9.790