Protein Alignment NKCC and SLC12A2

DIOPT Version :10

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: NP_001037.1
• Gene: SLC12A2 / 6558
• HGNCID: 10911
• Length: 1212
• Species: Homo sapiens
• Alignment Length: 1151
• Identity: 457/1151 - (39%)
• Similarity: 664/1151 - (57%)
• Gaps: 162/1151 - (14%)

  Fly     4 RFQISKVNGTANAGYDGEARSAESDQTDAAH---PLPPTSNEN----------HLHPKASGETGE 55
            ||:::.|:..|::       |||...:|||.   ..|..|.:|          .||....|.:|.
Human   138 RFRVNFVDPAASS-------SAEDSLSDAAGVGVDGPNVSFQNGGDTVLSEGSSLHSGGGGGSGH 195

  Fly    56 NR------RSSRLSFRGFGNFLRKSDAERKFSLAQLTKESLPRLDNYRISM----RNLKRPSIGE 110
            ::      .::....|.||:               .|.:::||:|:||.:.    ..|.|||:.|
Human   196 HQHYYYDTHTNTYYLRTFGH---------------NTMDAVPRIDHYRHTAAQLGEKLLRPSLAE 245

  Fly   111 LQGEAVDQSITIPEPEPEATG----------------------GHIKLGWIVGVLIPCLLNIWGV 153
            |..|.        |.||...|                      |.:|.|||.|||:.|:||||||
Human   246 LHDEL--------EKEPFEDGFANGEESTPTRDAVVTYTAESKGVVKFGWIKGVLVRCMLNIWGV 302

  Fly   154 MLFLRLSWVVAESGILQSLIIITISAVVCVITTLSLSAISTNGEVKGGGVYFIISRSLGPEFGAS 218
            |||:||||:|.::||..|:::|.::.||..||.||.|||:|||.|:|||.|::||||||||||.:
Human   303 MLFIRLSWIVGQAGIGLSVLVIMMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGA 367

  Fly   219 VGVVFAFANAVSASMNTIGFCESLNVLLKNNDLKIVDNGINDIRIVGSITVLVLILICCVGMEWE 283
            :|::|||||||:.:|..:||.|::..|||.:.:.::|. ||||||:|:|||::|:.|...|||||
Human   368 IGLIFAFANAVAVAMYVVGFAETVVELLKEHSILMIDE-INDIRIIGAITVVILLGISVAGMEWE 431

  Fly   284 TKAQNFLIVTIVLAIFNFLIGAAIGPQGNEEQISRGFVGFSWATLKENFGSDYRYAEGVNHDFFS 348
            .|||..|:|.::|||.:|:||..| |.  |.:..:||.|:......||||.|:|..|    .|||
Human   432 AKAQIVLLVILLLAIGDFVIGTFI-PL--ESKKPKGFFGYKSEIFNENFGPDFREEE----TFFS 489

  Fly   349 VFAIFFPSVTGIQAGANICGDLKDAGAAIPKGTFWSLLISMSSYALFVLFAGGAAVRDASGIPAD 413
            |||||||:.|||.|||||.|||.|..:||||||..::||:...|....:..|...||||:|
Human   490 VFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYVGIAVSVGSCVVRDATG---- 550

  Fly   414 LVNGTIVSSELPCMA-------------TGNCTWGLFNSYEMMQEMSLWGPLIYAGCFAATLSTA 465
            .||.|||:....|.:             :..|::||.|::::|..:|.:.|||.||.|:||||:|
Human   551 NVNDTIVTELTNCTSAACKLNFDFSSCESSPCSYGLMNNFQVMSMVSGFTPLISAGIFSATLSSA 615

  Fly   466 LTNLLSVPRLVQALGIDQIYPGLIFFSKPYGKHGEPYRGYVLTFFITTGFLLIGELNLIAPLIST 530
            |.:|:|.|::.|||..|.|||....|:|.|||:.||.|||:|||.|..||:||.|||:|||:||.
Human   616 LASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISN 680

  Fly   531 FYLASYALINFCTFHAAFVKPLGWRPTFKYYNAWLSLFGFAMCVAIMFLINYVAAIITFGIIFAL 595
            |:|||||||||..|||:..|..||||.|||||.|:||.|..:|..:||:||:.||::|:.|:..|
Human   681 FFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGL 745

  Fly   596 YLVVMYRKPEANWGSTTQAQQYKAALMAVHRLQNVSDHVKNYHPQVLVLSGDPKTRPPLVDFGYL 660
            |:.|.|:||:.||||:|||..|..||....||..|.|||||:.||.||::|.|.:||.|:...:.
Human   746 YIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHD 810

  Fly   661 LTKNNSLMFVANII--PVRVGYKNRQHLVKDGQKYLDARKIKAFYNVIDGFSLEDGINALTKSTG 723
            .|||..||...::.  |.|...|.........|::|...|:||||..:....|.:|...|.::.|
Human   811 FTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAG 875

  Fly   724 FGKMSPNIVLVGYKPDWNRCRKEEVESYFSILYNAFSQRMGVALLRLPNGLDFSELSSEVTLPAN 788
            .|:|.||.:::|:|.||.:....:|:.|.::.::||..:.||.::||..|||.|.|..:..|.:
Human   876 LGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQGQEELLS- 939

  Fly   789 GMGHMHTANAHGFTNELMPAANAASELLHIDSNLNLASMD-SPNSSFTMPQPAPMPNMQRNSRSY 852
                         :.|..|.....           :.|:: |..|.....:|     :.....::
Human   940 -------------SQEKSPGTKDV-----------VVSVEYSKKSDLDTSKP-----LSEKPITH 975

  Fly   853 KVTSSD-----EPAVTYHTKGGSDIP-----QNLLDAMTIFTRKQPKGTIDVFWLYDDGGLTILL 907
            ||...|     :|.:...:| |..:|     |.||:|.|.|.:||.|.||||:||:||||||:|:
Human   976 KVEEEDGKTATQPLLKKESK-GPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLI 1039

  Fly   908 PYIISMRSHWQNSKLRVFAMCHGKDE--EQEEKSMASLLTKFRIKYSELIMLKGVSEQPRADTVL 970
            ||:::.:..|::.|:|||  ..||..  :.:.::||:||:||||.:|::::|..::.:|:.:.::
Human  1040 PYLLTTKKKWKDCKIRVF--IGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENII 1102

  Fly   971 KHKRLIEPFRRGARNE-------------FGITDDELQSMSEKTNRQLRIHELVVKHSSNASLVV 1022
            ..:.:|||:|....::             :.|||:||:....||.||:|::||:.:|||.|:::|
Human  1103 AFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIV 1167

  Fly  1023 MSLPMPRKEAISAPLYMSWLEMLTSDMKCPVALARGNQTPVLTLYS 1068
            ||||:.||.|:|:.|||:|||.|:.|:. |:.|.|||...|||.||
Human  1168 MSLPVARKGAVSSALYMAWLEALSKDLP-PILLVRGNHQSVLTFYS 1212

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	AA_permease_2	85..1068	CDD:459263	435/1049 (41%)
SLC12A2	NP_001037.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	36..81
		RFXV motif 1. /evidence=ECO:0000269|PubMed:16669787	80..83
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	112..138		457/1151 (40%)
		RFXV motif 2. /evidence=ECO:0000269|PubMed:16669787	138..141		2/2 (100%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	150..193		12/49 (24%)
		2a30	216..1212	CDD:273347	435/1049 (41%)
		Scissor helix. /evidence=ECO:0000269|PubMed:35585053, ECO:0000269|PubMed:36306358, ECO:0007744|PDB:7MXO, ECO:0007744|PDB:7N3N, ECO:0007744|PDB:7S1X, ECO:0007744|PDB:7SFL, ECO:0007744|PDB:7SMP	761..778		7/16 (44%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	962..989		4/31 (13%)