Protein Alignment NKCC and SLC12A5

DIOPT Version :9

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: NP_001128243.1
• Gene: SLC12A5 / 57468
• HGNCID: 13818
• Length: 1139
• Species: Homo sapiens
• Alignment Length: 1313
• Identity: 318/1313 - (24%)
• Similarity: 504/1313 - (38%)
• Gaps: 419/1313 - (31%)

  Fly     1 MADRFQISKVNGTANAGYDGEARS--AESDQTDAAHPLPPTSNENHLHPKASGETGENRRSSRLS 63
            |:.||.::.:...      |.|||  .||.:...|.|       .||       .||:
Human     1 MSRRFTVTSLPPA------GPARSPDPESRRHSVADP-------RHL-------PGED------- 38

  Fly    64 FRGFGN------FLRKSDAER--------------KFSLAQLTKESLPRLDNYRISMRNLKRPSI 108
            .:|.||      |:..:|.|:              :...:.:....|..|.||    .||.:.|.
Human    39 VKGDGNPKESSPFINSTDTEKGKEYDGKNMALFEEEMDTSPMVSSLLSGLANY----TNLPQGSR 99

  Fly   109 GELQGEAVDQSITIPEPEPEATGGHIKLGWIVGVLIPCLLNIWGVMLFLRLSWVVAESGILQSLI 173
            ...:.|..:.....|...|       ::|..:||.:|||.||:||:|||||:|||..:||::|..
Human   100 EHEEAENNEGGKKKPVQAP-------RMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFC 157

  Fly   174 IITISAVVCVITTLSLSAISTNGEVKGGGVYFIISRSLGPEFGASVGVVFAFANAVSASMNTIGF 238
            ::.|.....::|.:|:|||:|||.|..||.|::||||||||||.:||:.|......:.:|..:|.
Human   158 MVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGT 222

  Fly   239 CESL-------NVLLKNNDLKIVDNG-----INDIRIVGSITVLVLILICCVGMEWETK-AQNFL 290
            .|.|       ..:.|..|.    :|     :|::|:.|:..:..:..:..||:::..| |..||
Human   223 IEILLAYLFPAMAIFKAEDA----SGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFL 283

  Fly   291 --IVTIVLAIFNFLIGAAIGP--------------------------QGNEEQISR--------- 318
              ::..:|||:..:|.:|..|                          :|||...:|
Human   284 GCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSR 348

  Fly   319 -------------------GFVGFSWATLKENFGSDY---------------RYAEG----VNH- 344
                               |..|.:...:|||..|.|               ..|:|    ::|
Human   349 FLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLTKGVIVERSGMTSVGLADGTPIDMDHP 413

  Fly   345 ----DFFSVF----AIFFPSVTGIQAGANICGDLKDAGAAIPKGTFWSL----LISMSSYALFVL 397
                |..|.|    .|:|||||||.||:|..|||:||..:||.||..::    .:.:||..||..
Human   414 YVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGA 478

  Fly   398 FAGGAAVRDASGIPADLVNGTIVSSELPCMATGNCTWGLFNSYEMMQEMSLWGPLIYAGCFAATL 462
            ...|..:||..|   :.|||.:|        .|...|           .|.|  :|..|.|.:|.
Human   479 CIEGVVLRDKFG---EAVNGNLV--------VGTLAW-----------PSPW--VIVIGSFFSTC 519

  Fly   463 STALTNLLSVPRLVQALGIDQIYPGLIFFSKPYGK-HGEPYRGYVLTFFITTGFLLIGELNLIAP 526
            ...|.:|...|||:||:..|.|.|.|..|.  :|| :|||....:||..|....:||..|:.:||
Human   520 GAGLQSLTGAPRLLQAISRDGIVPFLQVFG--HGKANGEPTWALLLTACICEIGILIASLDEVAP 582

  Fly   527 LISTFYLASYALINFCTFHAAFVKPLGWRPTFKYYNAWLSLFGFAMCVAIMFLINYVAAIITFGI 591
            ::|.|:|..|..:|........::...|||.|:||:..||..|.::|:|:||:.::..|::...|
Human   583 ILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLI 647

  Fly   592 IFALYLVVMYRKPEANWGSTTQAQQYKAALMAVHRLQNVSDHVKNYHPQVLVL----SGDPKTRP 652
            ...:|..:.||..|..||...:.....||..|:.||:....|.||:.||:|||    .......|
Human   648 AGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHP 712

  Fly   653 PLVDFGYLLTKNNSLMFVANII--PVRVGYKNRQHLVKDGQKYLDARKIKAFYNVIDGFSLEDGI 715
            .|:.....|.....|..|.:::  .....:...|...:..::.::|.|:|.|..|:...:|.||:
Human   713 QLLSLTSQLKAGKGLTIVGSVLEGTFLENHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGV 777

  Fly   716 NALTKSTGFGKMSPNIVLVGYKPDWNRCRKEEVESYFSILYNAFSQRMGVALLRLPNGLDFSELS 780
            :.|.:|.|.|.:..|.||||:..:|.  :||:.:::                      .:|.||.
Human   778 SHLIQSGGLGGLQHNTVLVGWPRNWR--QKEDHQTW----------------------RNFIELV 818

  Fly   781 SEVTLPANGMGHMHTANAHGFTNELMPAANAASELLHIDSNLNLASMDSPNSSFTMPQPAPMPNM 845
            .|.|     .||:
Human   819 RETT-----AGHL---------------------------------------------------- 826

  Fly   846 QRNSRSYKVTSSDEPAVTYHTKGGSDIPQNLLDAMTIFTRKQPKGTIDVFWLYDDGGLTILLPYI 910
                            ....||..|..|.|        ..:..:|:|||:|:..|||:.:|||::
Human   827 ----------------ALLVTKNVSMFPGN--------PERFSEGSIDVWWIVHDGGMLMLLPFL 867

  Fly   911 ISMRSHWQNSKLRVFAMCHGKDEE-QEEKSMASLLTKFRI-----------------KYSELIML 957
            :.....|:..|:|:|.:....|.. |.:|.:.:.|...||                 .|.:.:::
Human   868 LRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVM 932

  Fly   958 KGVS---------------------------------------------------EQPRADTVLK 971
            :..|                                                   |:|..:..|.
Human   933 EQRSQILKQMHLTKNEREREIQSITDESRGSIRRKNPANTRLRLNVPEETAGDSEEKPEEEVQLI 997

  Fly   972 HKRLI------------EPFRRGAR-------------------------NEFGITD-----DEL 994
            |.:..            ||...|..                         :..||.|     .|.
Human   998 HDQSAPSCPSSSPSPGEEPEGEGETDPEKVHLTWTKDKSVAEKNKGPSPVSSEGIKDFFSMKPEW 1062

  Fly   995 QSMSEKTNRQL----RIHELVVKHSSNASLVVMSLPMPRKEAISAPLYMSWLEMLTSDMKCPVAL 1055
            :::::...|::    |::|::||.|.:|.||::::|.|.:.......||.:||:||..:. .|.|
Human  1063 ENLNQSNVRRMHTAVRLNEVIVKKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEHLD-RVML 1126

  Fly  1056 ARGNQTPVLTLYS 1068
            .||....|:|:||
Human  1127 VRGGGREVITIYS 1139

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	2a30	85..1068	CDD:273347	295/1205 (24%)
		DUF502	507..>643	CDD:294696	44/135 (33%)
		SLC12	651..1068	CDD:281515	95/533 (18%)
SLC12A5	NP_001128243.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..63		21/88 (24%)
		2a30	44..1139	CDD:273347	299/1241 (24%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	92..116		5/23 (22%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	942..1052		7/109 (6%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0	Normalized mean entropy	S2236
OMA	1	1.010	-	-		QHG57166
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	3	2.870