Protein Alignment NKCC and slc12a5a

DIOPT Version :10

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: XP_073810821.1
• Gene: slc12a5a / 572215
• ZFINID: ZDB-GENE-120927-3
• Length: 1145
• Species: Danio rerio
• Alignment Length: 1317
• Identity: 306/1317 - (23%)
• Similarity: 506/1317 - (38%)
• Gaps: 421/1317 - (31%)

  Fly     1 MADRFQIS--KVNGTANAGYDGEARSAESDQTDAAHPLPPTSNENHLHPKASGETGENRRSSRLS 63
            |:.||.::  .:.|..|..::|:..:            |.|:       .|:|.:|
Zfish     1 MSQRFTVTTGDIQGEVNQLFEGDEET------------PSTA-------AAAGGSG--------- 37

  Fly    64 FRGFGN------FLRKSDAE-------RKFSLAQLTKESLPRLDNYRISMRNLKRPSIGELQGEA 115
             :|.||      |:..:|.|       :..:|.:...:::|.:.:...|:.|......|..:.|.
Zfish    38 -KGDGNPKESSPFINSTDPEKNQQFDGKNMALFEDEMDTIPMVSSLLSSLANYSNLPQGSKEHEE 101

  Fly   116 VDQ---SITIPEPEPEATGGHIKLGWIVGVLIPCLLNIWGVMLFLRLSWVVAESGILQSLIIITI 177
            .:.   |...|...|       ::|.::||.:|||.||.||:||||::|:|...|::::.||:.:
Zfish   102 AENNEGSRKKPVQAP-------RMGTLMGVYLPCLQNILGVILFLRMTWMVGIGGVIEAFIIVLM 159

  Fly   178 SAVVCVITTLSLSAISTNGEVKGGGVYFIISRSLGPEFGASVGVVFAFANAVSASMNTIGFCESL 242
            .....::|.:|:|||:|||.|..||.|::||||||||||.:||:.|......:.:|..:|..|.|
Zfish   160 CCSTTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGICFYLGTTYAGAMYILGCIEIL 224

  Fly   243 NV-------LLKNNDLKIVDNG---INDIRIVGSITVLVLILICCVGMEWETK-AQNFL--IVTI 294
            .|       :.|...|:..:..   :|::|:.|::.:..:.::..||:::..| |..||  ::..
Zfish   225 LVYIVPQAAIFKMEGLEGAEAEAAMLNNMRVYGTLVLSFMAIVVFVGVKYVNKLALVFLACVICS 289

  Fly   295 VLAIFNFLIGAAIGPQ-------GNEEQISRGF-------------------------------- 320
            :||::..:|..|..|.       ||...:.:||
Zfish   290 ILAVYAGVIKTAFEPPVFPVCVLGNRTLVWKGFDVCAKVIERENATVTTKLWRLFCDSEFLNATC 354

  Fly   321 ---------------VGFSWATLKENFGSDY--------------------------RYAEGVNH 344
                           .|....||:||...:|                          ||......
Zfish   355 DTYFTNNNVTEMQGIPGVMSGTLRENLFGNYMDKGAFIEKTGLSSVVEPDSIPTNTNRYVLADIT 419

  Fly   345 DFFSVF-AIFFPSVTGIQAGANICGDLKDAGAAIPKGTFWSL----LISMSSYALFVLFAGGAAV 404
            .||::. .|:|||||||.||:|..|||:||..:||.||..::    :|.|||..||.....|..:
Zfish   420 SFFTMLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPIGTILAITTTSIIYMSSVVLFGACIEGTVL 484

  Fly   405 RDASGIPADLVNGTIVSSELPCMATGNCTWGLFNSYEMMQEMSLWGPLIYAGCFAATLSTALTNL 469
            ||..|   :.|.|.:|        .|...|           .|.|  :|..|.|.:|....|.:|
Zfish   485 RDKFG---EAVRGNLV--------IGTLAW-----------PSPW--VIVIGSFFSTCGAGLQSL 525

  Fly   470 LSVPRLVQALGIDQIYPGLIFFSKPYGK-HGEPYRGYVLTFFITTGFLLIGELNLIAPLISTFYL 533
            ...|||:||:..|.|.|.|..|.  :|| :|||....:||..|....:||..|:.:||::|.|:|
Zfish   526 TGAPRLMQAIARDGIVPFLRVFG--HGKANGEPTWALLLTACICESGILIASLDNVAPILSMFFL 588

  Fly   534 ASYALINFCTFHAAFVKPLGWRPTFKYYNAWLSLFGFAMCVAIMFLINYVAAIITFGIIFALYLV 598
            ..|..:|........::...|||.||:|:..||..|.::|:::||:.::..||:..||...:|..
Zfish   589 MCYMFVNLACAVQTLLRTPNWRPRFKFYHWTLSFLGMSLCLSLMFICSWYYAIVAMGIAGCIYKY 653

  Fly   599 VMYRKPEANWGSTTQAQQYKAALMAVHRLQNVSDHVKNYHPQVLVL---SGDPKT-RPPLVDFGY 659
            :.:|..|..||...:.....||..|:.||:....|.||:.||:|||   .|:.|. :|.::....
Zfish   654 IEFRGAEKEWGDGIRGLSLSAARFALMRLEEGPPHTKNWRPQILVLVNMDGELKVDQPRMLSLTS 718

  Fly   660 LLTKNNSLMFVANIIPVRV------GYKNRQHLVKDGQKYLDARKIKAFYNVIDGFSLEDGINAL 718
            .|.....|..|...:....      |.:..|.|    :|.::..|:|.|..|:...::.|.::.|
Zfish   719 QLKAGKGLTIVGTALEGTYLENHDQGQQAEQSL----RKLMETEKVKGFSQVVISSNMRDAMSHL 779

  Fly   719 TKSTGFGKMSPNIVLVGYKPDWNRCRKEEVESYFSILYNAFSQRMGVALLRLPNGLDFSELSSEV 783
            .:::|.|.:..|.||..:..:|.:.  |:.:|:                      .:|.||..|.
Zfish   780 VQASGLGGLQHNTVLASWPRNWKQA--EDHQSW----------------------RNFIELVRET 820

  Fly   784 TLPANGMGHMHTANAHGFTNELMPAANAASELLHIDSNLNLASMDSPNSSFTMPQPAPMPNMQRN 848
            |                          |.|:.|.:..  |:::..|....||
Zfish   821 T--------------------------AGSKALLVPK--NISAFPSNGERFT------------- 844

  Fly   849 SRSYKVTSSDEPAVTYHTKGGSDIPQNLLDAMTIFTRKQPKGTIDVFWLYDDGGLTILLPYIISM 913
                                                    :|.|||:|:..|||:.:|||:::..
Zfish   845 ----------------------------------------EGHIDVWWIVHDGGMLMLLPFLLRQ 869

  Fly   914 RSHWQNSKLRVFAMCHGKDEE-QEEKSMASLLTKFRI-----------------KYSELIMLK-- 958
            ...|:..|:|:|.:....|.. |.:|.:.:.|...||                 .|.:.:|::
Zfish   870 HKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRIDAAVEVVEMHDSDISAYTYEKTLMMEQR 934

  Fly   959 -----------------------------------------GVSEQPRADT-------------- 968
                                                     .|:|:|.|.:
Zfish   935 SQILKQINLTKTEREREIQSITDSSRGSIRRKNPAAVTTQLSVTEEPAASSKEQKPEEECVPVSP 999

  Fly   969 ----VLKHKRLIEPFR---------------------------RGARNE-FGITDDEL------- 994
                .|.|.:...|..                           .|.:|: .|.|..|.
Zfish  1000 SQQEQLIHDKSTNPPTPATPQSPSADAQTPGTAVQMTWSEVKCDGEQNKPVGATTPEAIKDLFNM 1064

  Fly   995 --------QSMSEKTNRQLRIHELVVKHSSNASLVVMSLPMPRKEAISAPLYMSWLEMLTSDMKC 1051
                    ||...:.:..||::|:::|.|..|.||::::|.|.:.......||.::|:||..:.
Zfish  1065 KPEWENLNQSNVRRMHTALRLNEVIIKKSQEAKLVLLNMPGPPRNRSGDENYMEFMEVLTEGLN- 1128

  Fly  1052 PVALARGNQTPVLTLYS 1068
            .|.|.||....|:|:||
Zfish  1129 RVLLVRGGGREVITIYS 1145

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	AA_permease_2	85..1068	CDD:459263	286/1216 (24%)
slc12a5a	XP_073810821.1	None