DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment NKCC and Slc12a5

DIOPT Version :9

Sequence 1:	NP_730938.1	Gene:	NKCC / 40663	FlyBaseID:	FBgn0051547	Length:	1068	Species:	Drosophila melanogaster
Sequence 2:	NP_001342409.1	Gene:	Slc12a5 / 57138	MGIID:	1862037	Length:	1138	Species:	Mus musculus

Alignment Length:	1281	Identity:	310/1281 - (24%)
Similarity:	496/1281 - (38%)	Gaps:	400/1281 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly    36 LPPTSNENHLHPKASGET-GENRRSSRLSFRGFGN------FLRKSDAE-------RKFSLAQLT 86
            |||.::.....|::...: .:.||..|...:|.||      |:..:|.|       |..:|.:..
Mouse    10 LPPAASAASADPESRRHSVADPRRLPREDVKGDGNPKESSPFINSTDTEKGREYDGRNMALFEEE 74

  Fly    87 KESLPRLDNYRISMRNLKRPSIGELQGEAVDQSITIPEPEPEATGGHIK------LGWIVGVLIP 145
            .::.|.:.:....:.|......|..:.|..:.:          .||..|      :|..:||.:|
Mouse    75 MDTSPMVSSLLSGLANYTNLPQGSREHEEAENN----------EGGKKKPVQAPRMGTFMGVYLP 129

  Fly   146 CLLNIWGVMLFLRLSWVVAESGILQSLIIITISAVVCVITTLSLSAISTNGEVKGGGVYFIISRS 210
            ||.||:||:|||||:|||..:||::|..::.|.....::|.:|:|||:|||.|..||.|::||||
Mouse   130 CLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRS 194

  Fly   211 LGPEFGASVGVVFAFANAVSASMNTIGFCESL-------NVLLKNNDLKIVDNG-----INDIRI 263
            ||||||.:||:.|......:.:|..:|..|.|       ..:.|..|.    :|     :|::|:
Mouse   195 LGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDA----SGEAAAMLNNMRV 255

  Fly   264 VGSITVLVLILICCVGMEWETK-AQNFL--IVTIVLAIFNFLIGAAIGP---------------- 309
            .|:..:..:..:..||:::..| |..||  ::..:|||:..:|.:|..|
Mouse   256 YGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRH 320

  Fly   310 ----------QGNEEQISR----------------------------GFVGFSWATLKENFGSDY 336
                      :|||...:|                            |..|.:...:|||..|.|
Mouse   321 GFDVCAKLAWEGNETVTTRLWGLFCSSRLLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSY 385

  Fly   337 ---------------RYAEG----VNH-----DFFSVF----AIFFPSVTGIQAGANICGDLKDA 373
                           ..|:|    ::|     |..|.|    .|:|||||||.||:|..|||:||
Mouse   386 LTKGVIVERRGMPSVGLADGTPVDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDA 450

  Fly   374 GAAIPKGTFWSL----LISMSSYALFVLFAGGAAVRDASGIPADLVNGTIVSSELPCMATGNCTW 434
            ..:||.||..::    .:.:||..||.....|..:||..|   :.|||.:|        .|...|
Mouse   451 QKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFG---EAVNGNLV--------VGTLAW 504

  Fly   435 GLFNSYEMMQEMSLWGPLIYAGCFAATLSTALTNLLSVPRLVQALGIDQIYPGLIFFSKPYGK-H 498
                       .|.|  :|..|.|.:|....|.:|...|||:||:..|.|.|.|..|.  :|| :
Mouse   505 -----------PSPW--VIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFG--HGKAN 554

  Fly   499 GEPYRGYVLTFFITTGFLLIGELNLIAPLISTFYLASYALINFCTFHAAFVKPLGWRPTFKYYNA 563
            |||....:||..|....:||..|:.:||::|.|:|..|..:|........::...|||.|:||:.
Mouse   555 GEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHW 619

  Fly   564 WLSLFGFAMCVAIMFLINYVAAIITFGIIFALYLVVMYRKPEANWGSTTQAQQYKAALMAVHRLQ 628
            .||..|.::|:|:||:.::..|::...|...:|..:.||..|..||...:.....||..|:.||:
Mouse   620 TLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLE 684

  Fly   629 NVSDHVKNYHPQVLVL----SGDPKTRPPLVDFGYLLTKNNSLMFVANIIPVRV--GYKNRQHLV 687
            ....|.||:.||:|||    .......|.|:.....|.....|..|.:::....  .:...|...
Mouse   685 EGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLDNHPQAQRAE 749

  Fly   688 KDGQKYLDARKIKAFYNVIDGFSLEDGINALTKSTGFGKMSPNIVLVGYKPDWNRCRKEEVESYF 752
            :..::.::|.|:|.|..|:...:|.||::.|.:|.|.|.:..|.||||:..:|.  :||:.:::
Mouse   750 ESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWR--QKEDHQTW- 811

  Fly   753 SILYNAFSQRMGVALLRLPNGLDFSELSSEVTLPANGMGHMHTANAHGFTNELMPAANAASELLH 817
                                 .:|.||..|.|     .||:
Mouse   812 ---------------------RNFIELVRETT-----AGHL------------------------ 826

  Fly   818 IDSNLNLASMDSPNSSFTMPQPAPMPNMQRNSRSYKVTSSDEPAVTYHTKGGSDIPQNLLDAMTI 882
                                                        ....||..|..|.|
Mouse   827 --------------------------------------------ALLVTKNVSMFPGN------- 840

  Fly   883 FTRKQPKGTIDVFWLYDDGGLTILLPYIISMRSHWQNSKLRVFAMCHGKDEE-QEEKSMASLLTK 946
             ..:..:|:|||:|:..|||:.:|||:::.....|:..|:|:|.:....|.. |.:|.:.:.|..
Mouse   841 -PERFSEGSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYH 904

  Fly   947 FRI-----------------KYSELIMLKGVS--------------------------------- 961
            .||                 .|.:.::::..|
Mouse   905 LRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDESRGSIRRKNP 969

  Fly   962 ------------------EQPRADTVLKHKRLI------------EPFRRGARNEFGITDDEL-- 994
                              |:|..:..|.|.:..            ||  .|.|.    ||.|:
Mouse   970 ANPRLRLNVPEETACDNEEKPEEEVQLIHDQSAPSCPSSSPSPGEEP--EGERE----TDPEVHL 1028

  Fly   995 -----QSMSEKT--------------------------------NRQLRIHELVVKHSSNASLVV 1022
                 :|::||.                                :..:|::|::|..|.:|.||:
Mouse  1029 TWTKDKSVAEKNKGPSPVSSEGIKDFFSMKPEWENLNQSNVRRMHTAVRLNEVIVNKSRDAKLVL 1093

  Fly  1023 MSLPMPRKEAISAPLYMSWLEMLTSDMKCPVALARGNQTPVLTLYS 1068
            :::|.|.:.......||.:||:||..:. .|.|.||....|:|:||
Mouse  1094 LNMPGPPRNRNGDENYMEFLEVLTEQLD-RVMLVRGGGREVITIYS 1138

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	2a30	85..1068	CDD:273347	293/1216 (24%)
		DUF502	507..>643	CDD:294696	44/135 (33%)
		SLC12	651..1068	CDD:281515	96/538 (18%)
Slc12a5	NP_001342409.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..62		13/51 (25%)
		2a30	44..1138	CDD:273347	299/1245 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	96..116		5/29 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	943..1051		14/113 (12%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0	Normalized mean entropy	S2236
OMA	1	1.010	-	-		QHG57166
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.870