Protein Alignment NKCC and slc12a3

DIOPT Version :10

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: NP_001038545.1
• Gene: slc12a3 / 565439
• ZFINID: ZDB-GENE-030131-9505
• Length: 1012
• Species: Danio rerio
• Alignment Length: 1089
• Identity: 420/1089 - (38%)
• Similarity: 603/1089 - (55%)
• Gaps: 157/1089 - (14%)

  Fly    56 NRRSSRLSFRGFGNFLRKSDAERKFSLAQL--------------------TKESLPRLDNYR--- 97
            |.:|:|..|.  .:.|...| :|||||...                    |.::.|..|.|.
Zfish     2 NLQSARSPFS--VSSLSNGD-DRKFSLGGCSSYDTDSVASRSSGVFSGYDTLDAPPSYDFYTNTE 63

  Fly    98 -ISMRNLKRPSIGELQGEAVDQSITIP-------------------EPEPEATGGHIKLGWIVGV 142
             .......|||:.||.....|.....|                   ||.||..    :.||..||
Zfish    64 VFGRAKKSRPSLFELHSNPQDDPSPPPLYEESSVDKQSPEDEDEPTEPPPEPA----RFGWAQGV 124

  Fly   143 LIPCLLNIWGVMLFLRLSWVVAESGILQSLIIITISAVVCVITTLSLSAISTNGEVKGGGVYFII 207
            :|.|:||||||:|:|||.|:.|::||..:.|||.:|:.:..||.||.|||:|||:|||||.||:|
Zfish   125 MIRCMLNIWGVILYLRLPWITAQAGIGLTWIIILVSSSITGITGLSTSAIATNGKVKGGGTYFLI 189

  Fly   208 SRSLGPEFGASVGVVFAFANAVSASMNTIGFCESLNVLLKNNDLKIVDNGINDIRIVGSITVLVL 272
            |||||||.|.|:|::|||||||:.:|:|:||.|::.||::..::.:||. :|||||:|.|||..|
Zfish   190 SRSLGPELGGSIGLIFAFANAVAVAMHTVGFAETVQVLMQETEVSMVDK-LNDIRIIGVITVTCL 253

  Fly   273 ILICCVGMEWETKAQNFLIVTIVLAIFNFLIGAAIGPQGNEEQISRGFVGFSWATLKENFGSDYR 337
            :.|...|||||:|||......|:::..:::||..| |...::| :|||..:.......||...:|
Zfish   254 LAISMAGMEWESKAQVLFFFVIMISFASYIIGTII-PATPQKQ-ARGFFSYRADIFATNFVPGWR 316

  Fly   338 YAEGVNHDFFSVFAIFFPSVTGIQAGANICGDLKDAGAAIPKGTFWSLLISMSSYALFVLFAGGA 402
            ..||   .||.:|:|||||.|||.|||||.|||||...|||:||..::..:..||.:.....|..
Zfish   317 GPEG---SFFGMFSIFFPSATGILAGANISGDLKDPNVAIPRGTMLAIFWTTVSYLIISATIGSC 378

  Fly   403 AVRDASGIPADLVNGTIVS--------------SELPCMATGNCTWGLFNSYEMMQEMSLWGPLI 453
            .||||||.    ||.||.|              :...||....||:||.|.|:.|..:|...|||
Zfish   379 VVRDASGD----VNDTISSLTGECLGVGCNYGWNFTDCMTNNTCTYGLSNYYQSMSMVSAVAPLI 439

  Fly   454 YAGCFAATLSTALTNLLSVPRLVQALGIDQIYPGLIFFSKPYGKHGEPYRGYVLTFFITTGFLLI 518
            .||.|.||||:||..|:|.|::.|.|..|::|||:.||.|.|||:.||.|.|:|.:.|...|:||
Zfish   440 TAGIFGATLSSALACLVSAPKVFQCLCKDKLYPGIGFFGKGYGKNNEPLRSYLLAYIIAICFILI 504

  Fly   519 GELNLIAPLISTFYLASYALINFCTFHAAFVKPLGWRPTFKYYNAWLSLFGFAMCVAIMFLINYV 583
            .|||.|||:||.|:|.|||||||..|||:.....||||||::|:.||||.|..:.|.||||:.:.
Zfish   505 AELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPTFRFYSKWLSLLGAVVSVIIMFLLTWW 569

  Fly   584 AAIITFGIIFALYLVVMYRKPEANWGSTTQAQQYKAALMAVHRLQNVSDHVKNYHPQVLVLSGDP 648
            ||:|..||:..|...|:|:|||.||||:.||..|..||.....|..|.||:|||.||.|||||.|
Zfish   570 AALIAIGIVIFLLGYVLYKKPEVNWGSSMQASSYNMALSQCVGLNQVEDHIKNYRPQCLVLSGPP 634

  Fly   649 KTRPPLVDFGYLLTKNNSLMFVANII-----PVRVGYKNRQHLVKDGQKYLDARKIKAFYNVIDG 708
            ..||.||||....|||.|||..||::     |......:..||     |:|:.||||:||:.:..
Zfish   635 CARPSLVDFIGAFTKNQSLMICANVLASGPSPGTADSMSSTHL-----KWLNNRKIKSFYHTVVA 694

  Fly   709 FSLEDGINALTKSTGFGKMSPNIVLVGYKPDWNRCRKEEVESYFSILYNAFSQRMGVALLRLPNG 773
            ..|..|:..|.:|||.|:|.||::::|||.:|.:.:...:|:|..||::||..:.||.:||:..|
Zfish   695 DDLRTGVQMLLQSTGLGRMKPNVLVMGYKKNWRKVQPGIIENYVGILHDAFDLQYGVCVLRMKEG 759

  Fly   774 LDFSE-LSSEVTLPANGMGHMHTANAHGF-TNELMPAANAASELLHIDSNLNLASMDSPNSSFTM 836
            ||.:. :.::|.|      ...|:...|. ||...|.:....           ||:| |.:...:
Zfish   760 LDITRTIQAQVNL------GFETSTEQGLDTNSTAPTSPTIE-----------ASLD-PETLMAL 806

  Fly   837 PQPAPMPNMQRNSRSYKVTSSDEPAVTYHTKGGSDIPQNLLDAMTIFTRKQPKGTIDVFWLYDDG 901
            .||:                                        |:|..:|.|.||||:||.|||
Zfish   807 TQPS----------------------------------------TLFQTRQGKKTIDVYWLSDDG 831

  Fly   902 GLTILLPYIISMRSHWQNSKLRVFAMCHGKDEEQEEKSMASLLTKFRIKYSELIMLKGVSEQPRA 966
            |||:|:||:::.:..|...|:|||.....:..|:::|.:..|:::||:.:.::.:|..::..|::
Zfish   832 GLTLLIPYLLTRKKRWGRCKVRVFVGGEAQQIEEQKKELKGLISRFRLGFKDIQVLPDINGAPQS 896

  Fly   967 DTVLKHKRLIEPFRRG-----------ARNEFG--ITDDELQSMSEKTNRQLRIHELVVKHSSNA 1018
            :.:.|.:..|.|:|..           |..||.  ::|:|:::...|:.||:|::|::..:|.:|
Zfish   897 EHIRKFEDFIAPYRVSSVQKDGQEADEATKEFSWMVSDEEMETFKAKSLRQIRLNEVIQDYSRDA 961

  Fly  1019 SLVVMSLPMPRKEAISAPLYMSWLEMLTSDMKCPVALARGNQTPVLTLY 1067
            :|:|:::|:.|:.:..:||||:|||:::.|::.||.|.||||..|||.|
Zfish   962 ALIVVTMPVGRRGSCPSPLYMAWLEIVSRDLRPPVLLVRGNQENVLTQY 1010

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	AA_permease_2	85..1068	CDD:459263	409/1060 (39%)
slc12a3	NP_001038545.1	2a30	48..1011	CDD:273347	409/1040 (39%)