Protein Alignment NKCC and slc12a10.2

DIOPT Version :9

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: NP_001038466.1
• Gene: slc12a10.2 / 562834
• ZFINID: ZDB-GENE-060503-425
• Length: 998
• Species: Danio rerio
• Alignment Length: 1066
• Identity: 409/1066 - (38%)
• Similarity: 596/1066 - (55%)
• Gaps: 135/1066 - (12%)

  Fly    54 GENRRSSRLSFR-------GFGNFLRKSDAERKFSLAQLTKESLPRLDNY-------RISMRNLK 104
            |..||.|.:..:       |..:|.:|.:  :|.|:.. ..:.:|:.|.|       ||..   .
Zfish    14 GMRRRDSNVPHQGDVAVDMGEADFGKKEN--QKMSIYN-PLDPVPKYDFYAKDTWAGRIKK---S 72

  Fly   105 RPSIGELQGEAVDQSITIPEPEPEATGGHIKL---GWIVGVLIPCLLNIWGVMLFLRLSWVVAES 166
            |||:..|:....|  || |.|:.|.....:||   ||::||.|.|:||||||:::|||.||.:::
Zfish    73 RPSLDVLRNPPDD--IT-PTPDEEDGRPKVKLVRFGWVLGVWIRCMLNIWGVIMYLRLPWVTSQA 134

  Fly   167 GILQSLIIITISAVVCVITTLSLSAISTNGEVKGGGVYFIISRSLGPEFGASVGVVFAFANAVSA 231
            |::.:.:||.:|..:..||..|:|||||||:|..||.||:||||||||.||.:|::||||.:::.
Zfish   135 GLVLTFVIIFMSVTITTITATSVSAISTNGKVYSGGTYFMISRSLGPELGAPIGLLFAFACSIAC 199

  Fly   232 SMNTIGFCESLNVLLKNNDLKIVDNGINDIRIVGSITVLVLILICCVGMEWETKAQNFLIVTIVL 296
            :::|:||.|::..:||:...::||: :||:||:|:|||.:.:||...||.||.|||....:.|:|
Zfish   200 ALHTVGFSETVRDMLKDFKSQMVDD-VNDVRIIGAITVTICLLITFAGMAWEAKAQILFFIAIIL 263

  Fly   297 AIFNFLIGAAIGPQGNEEQISRGFVGFSWATLKENFGSDYRYAEGVNHDFFSVFAIFFPSVTGIQ 361
            ::.|:.:|..|.|...:|.:  ||.|:.......|....:|   |.:..|..|||||||:.|||.
Zfish   264 SLLNYFVGTVIPPTQEKEAV--GFFGYHSDIFVSNLLPSFR---GPSGSFLVVFAIFFPACTGIL 323

  Fly   362 AGANICGDLKDAGAAIPKGTFWSLLISMSSYALFVLFAGGAAVRDASGIPAD---LVNGTIVSSE 423
            :|.||||||||....|||||..|:|.:..||.|.........||||||...|   :.|.||..|.
Zfish   324 SGVNICGDLKDPTGGIPKGTLLSILCTTLSYLLIAATCAATVVRDASGNKNDSLAISNYTIHCSG 388

  Fly   424 L---------PCMATGNCTWGLFNSYEMMQEMSLWGPLIYAGCFAATLSTALTNLLSVPRLVQAL 479
            |         .|....|||.||.|:..::..:|.||.||..|.||||||:||..|:|.|::.|.|
Zfish   389 LGCKYGWNFDSCEQYRNCTQGLANNLRILTVVSGWGILITIGTFAATLSSALGFLVSAPKVFQLL 453

  Fly   480 GIDQIYPGLIFFSKPYGKHGEPYRGYVLTFFITTGFLLIGELNLIAPLISTFYLASYALINFCTF 544
            ..|:|||.:.||.|.|||:.||.|.|||||.|...|:|||.||.||||||.|:||||.||||..|
Zfish   454 CKDKIYPYIGFFGKGYGKNKEPLRAYVLTFIIAMCFILIGNLNTIAPLISNFFLASYGLINFSCF 518

  Fly   545 HAAFVKPLGWRPTFKYYNAWLSLFGFAMCVAIMFLINYVAAIITFGIIFALYLVVMYRKPEANWG 609
            ||:..|..||||.::||:.|||||...:...:|||..:.||::||.::..|...|.|:|...|||
Zfish   519 HASITKSPGWRPQYRYYSPWLSLFCCCLSFTLMFLFTWWAALVTFSVVLFLIGYVNYKKTYVNWG 583

  Fly   610 STTQAQQYKAALMAVHRLQNVSDHVKNYHPQVLVLSGDPKTRPPLVDFGYLLTKNNSLMFVANI- 673
            |:.||..|..||.....|..|.|||||:.||.|||||.|.|||.||||....|||.|||...||
Zfish   584 SSYQAAFYNMALNFSMNLTTVPDHVKNFRPQCLVLSGPPNTRPALVDFVGTFTKNVSLMICGNIT 648

  Fly   674 -------IPVRVGYKNRQHLVKDGQKYLDARKIKAFYNVIDGFSLEDGINALTKSTGFGKMSPNI 731
                   .|     ::...||.:   :|..||:::||......||:||.:.|..::|.||:.||.
Zfish   649 IEGEQSDFP-----EHSSDLVVE---WLTKRKVRSFYTTFAAESLQDGAHNLMLASGLGKLKPNT 705

  Fly   732 VLVGYKPDWNRCRKEEVESYFSILYNAFSQRMGVALLRLPNGLDFSELSSEVTLPANGMGHMHTA 796
            :::|||.:|..|:.|.::.|.:.:.:||....||||||:.:.||
Zfish   706 LVLGYKTNWQECKPESLQDYINTINDAFDSNYGVALLRMMDKLD--------------------- 749

  Fly   797 NAHGFTNELMPAANAASELLHIDSNLNLASMDSPNSSF---TMPQPAPMPNMQRNSRSYKVTSSD 858
                .|:::..|.:            .|:::|:|  :|   .:|| :....:.|:|.:....|||
Zfish   750 ----ITDDMYSAED------------GLSAIDNP--AFERDEVPQ-SRSDTIDRDSDNSDEESSD 795

  Fly   859 EPAVTYHTKGGSDIPQNLLDAMTIFTRKQPKGTIDVFWLYDDGGLTILLPYIISMRSHWQNSKLR 923
            :                   ..|:|..||.|.|||::|:.||||||:|:||:::.|:.|:.||:|
Zfish   796 Q-------------------IRTVFQAKQGKKTIDIYWISDDGGLTLLVPYLLTRRNRWKKSKIR 841

  Fly   924 VFAMCHGKDEEQEEKSMASLLTKFRIKYSELIMLKGVSEQPRADTVLKHKRLIEPFRRGAR---- 984
            ||.:...:.::::...|..||.:||::..|:|::..|.:.|.|..|.:.:..|.|||....
Zfish   842 VFILGDQETKKEDRDDMKMLLKRFRLEIEEVIVITDVDKPPLAKNVQRFEDTITPFRLSEEQTRV 906

  Fly   985 --------NEFGITDDELQSMSEKTNRQLRIHELVVKHSSNASLVVMSLPMPRKEAISAPLYMSW 1041
                    |.:.::|.:|:::..|..|.:|::|::.|:|..|:|||:|||:|.....|: |||:|
Zfish   907 DLQEFRRLNPWAVSDKDLEAVRPKVERTVRLNEIIKKNSLYAALVVVSLPVPDLNCPSS-LYMAW 970

  Fly  1042 LEMLTSDMKCPVALARGNQTPVLTLY 1067
            ::.|:..:.||..|.||||..|:|.|
Zfish   971 MDALSIGINCPALLIRGNQENVMTFY 996

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	2a30	85..1068	CDD:273347	400/1028 (39%)
		DUF502	507..>643	CDD:294696	69/135 (51%)
		SLC12	651..1068	CDD:281515	135/440 (31%)
slc12a10.2	NP_001038466.1	AA_permease_N	48..98	CDD:285588	15/56 (27%)
		2a30	49..997	CDD:273347	400/1029 (39%)
		SLC5-6-like_sbd	125..556	CDD:294310	203/436 (47%)
		SLC12	625..997	CDD:281515	135/440 (31%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG2083
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	817	1.000	Inparanoid score	I458
OMA	1	1.010	-	-		QHG57166
OrthoDB	1	1.010	-	-		D135130at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm6412
orthoMCL	1	0.900	-	-		OOG6_101360
Panther	1	1.100	-	-	O	PTHR11827
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	10	9.840