Protein Alignment NKCC and slc12a4

DIOPT Version :10

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: XP_691291.2
• Gene: slc12a4 / 562826
• ZFINID: ZDB-GENE-090218-27
• Length: 1095
• Species: Danio rerio
• Alignment Length: 1220
• Identity: 305/1220 - (25%)
• Similarity: 496/1220 - (40%)
• Gaps: 357/1220 - (29%)

  Fly    52 ETGENR---RSSRLSFRGFGN------FLRKSDA---------------ERKFSLAQLTKESLPR 92
            :||:..   ....:|..|.||      ||..||:               |.:..:.......|.|
Zfish    30 DTGQGEYPGHGDTVSSDGHGNHKEDSPFLSSSDSASKRNDFYDRNLALFEEELDIRPKVSSLLSR 94

  Fly    93 LDNYRISMRNLKRPSIGELQGEAVDQSITIPEPEPEATGGHIKLGWIVGVLIPCLLNIWGVMLFL 157
            |.||    .|:.:.:....:.|:.|.|.......|       .:|.::||.:|||.||:||:|||
Zfish    95 LVNY----TNVTQGAKEHEEAESADASRRQAPKSP-------NMGTLMGVYLPCLQNIFGVILFL 148

  Fly   158 RLSWVVAESGILQSLIIITISAVVCVITTLSLSAISTNGEVKGGGVYFIISRSLGPEFGASVGVV 222
            ||:|:|..:|::||.:|:.:.....::|.:|:|||:|||.|..||.||:||||||||||.:||:.
Zfish   149 RLTWIVGTAGVVQSFLIVLMCCSCTMLTAISMSAIATNGVVPAGGAYFMISRSLGPEFGGAVGLC 213

  Fly   223 FAFANAVSASMNTIGFCESLNVLLK----------NNDLKIVDNG--INDIRIVGSITVLVLILI 275
            |......:|:|..:|   ::.:.||          ..|:. ..||  :|::|:.|:|.:.::.::
Zfish   214 FYLGTTFAAAMYILG---AIEIFLKYLIPQAAIFHPADIH-AGNGAMLNNMRVYGTICLSLMAVV 274

  Fly   276 CCVGMEWETKAQNFLIVTIVLAIFNFLIGAA-------------IGPQ----------------G 311
            ..||:::..|..:..:..::::|.:...||.             :|.:                |
Zfish   275 VFVGVKYVNKLASLFLACVIISIVSIYAGAVKSIFHPPEFPICMLGNRTLVRDLFDVCGKTVVVG 339

  Fly   312 NE-------------EQIS----------------RGFVGFSWATLKENFGSDY----------- 336
            ||             :.||                :|..|.:...:|||...||
Zfish   340 NETVPSKLWKNFCTSDNISSAHCDEYFFHNNVTEIKGIPGLASGIIKENMWGDYMEKGQILEKAK 404

  Fly   337 -----------RYAEGVNHDFFSVF----AIFFPSVTGIQAGANICGDLKDAGAAIPKGTFWSL- 385
                       .:...|:.|..:.|    .|||||.|||.||:|..|||:||..:||.||..::
Zfish   405 LPSVDVHGSMETFGFYVSADIATSFTLLVGIFFPSATGIMAGSNRSGDLRDAQKSIPIGTILAIT 469

  Fly   386 ---LISMSSYALFVLFAGGAAVRDASGIPADLVNGTIVSSELPCMATGNCTWGLFNSYEMMQEMS 447
               |:..||..||.....|..:||..|   |.|:..:|        .|..:|           .|
Zfish   470 TTSLVYFSSVVLFGACIEGVVLRDKFG---DAVSKNLV--------VGTLSW-----------PS 512

  Fly   448 LWGPLIYAGCFAATLSTALTNLLSVPRLVQALGIDQIYPGLIFFSKPYGK-HGEPYRGYVLTFFI 511
            .|  :|..|.|.:|:...|.:|...|||:||:..|.|.|.|..|.  :|| :|||....:||..|
Zfish   513 PW--VIVIGSFFSTVGAGLQSLTGAPRLLQAIAKDNIIPFLRVFG--HGKANGEPTWALLLTGLI 573

  Fly   512 TTGFLLIGELNLIAPLISTFYLASYALINFCTFHAAFVKPLGWRPTFKYYNAWLSLFGFAMCVAI 576
            ....:||..|:::||::|.|:|..|..:|........::...|||.|:||:..||..|.:||:|:
Zfish   574 AELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFRYYHWALSFLGMSMCLAL 638

  Fly   577 MFLINYVAAIITFGIIFALYLVVMYRKPEANWGSTTQAQQYKAALMAVHRLQNVSDHVKNYHPQV 641
            ||:.::..||:..||...:|..:.|:..|..||...:.....||..::.||:....|.||:.||:
Zfish   639 MFISSWYYAIVAMGIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYSLLRLEAGPPHTKNWRPQL 703

  Fly   642 LV---LSGDPKTR-PPLVDFGYLLTKNNSLMFVANIIPVRVG-----YKNRQHLVKDGQKYLDAR 697
            ||   |..|...: |.|:.|...|.....|..|.::|.   |     |...|...:..:..::..
Zfish   704 LVLLKLDEDLHVKYPRLLTFASQLKAGKGLTIVGSVIQ---GNFLECYGETQASEQAIKNMMEIE 765

  Fly   698 KIKAFYNVIDGFSLEDGINALTKSTGFGKMSPNIVLVGYKPDWNRCRKEEVESYFSILYNAFSQR 762
            ::|.|..|:....:.:||..|.:|.|.|.|..|.|::|:...|   |:.|....:....|.
Zfish   766 RVKGFCQVVVASKVREGIAHLIQSCGLGGMKHNTVVMGWPYGW---RQSEDPRAWKTFINT---- 823

  Fly   763 MGVALLRLPNGLDFSELSSEVTLPANGMGHMHTANAHGFTNELMPAANAASELLHIDSNLNLASM 827
                 :|.                        |..||                        ||.|
Zfish   824 -----VRC------------------------TTAAH------------------------LALM 835

  Fly   828 DSPNSSFTMPQPAPMPNMQRNSRSYKVTSSDEPAVTYHTKGGSDIPQNLLDAMTIFTRKQPKGTI 892
            ...|.||   .|:   |.:|                                   ||    .|.|
Zfish   836 VPKNVSF---YPS---NHER-----------------------------------FT----DGYI 855

  Fly   893 DVFWLYDDGGLTILLPYIISMRSHWQNSKLRVFAMCHGKDEE-QEEKSMASLLTKFRIK------ 950
            ||:|:..|||:.:|||:::.....|:..|:|:|.:....|.. |.:|.:|:.|.:.|::
Zfish   856 DVWWIVHDGGMLMLLPFLLKQHKVWRKCKMRIFTVAQMDDNSIQMKKDLATFLYQLRLEAEVEVV 920

  Fly   951 -----------YSELIMLK-----------GVSEQPRADTVLKHKRLIEPFRRGA---------- 983
                       |...:|::           ..:|:.|...::|.:..:  .|.|:
Zfish   921 EMHDSDISAYTYERTLMMEQRSQMLKQMRLSSAERDREAQLVKDRHSL--IRMGSLYSDEEEETI 983

  Fly   984 -----RNEFGITDDELQSMSEKTNRQ-------------------------LRIHELVVKHSSNA 1018
                 :|:...|.::::  :|:.||.                         ::::|::|..|.:|
Zfish   984 EILPEKNQMTWTSEKIE--AERRNRNNAPENFRELISIKPDQSNVRRMHTAVKLNEVIVNKSHDA 1046

  Fly  1019 SLVVMSLPMPRKEAISAPLYMSWLEMLTSDMKCPVALARGNQTPVLTLYS 1068
            .||::::|.|.:.......||.:||:||..:: .|.|.||....|:|:||
Zfish  1047 RLVLLNMPGPPRNQEGDENYMEFLEVLTEGLE-RVLLVRGGGREVITIYS 1095

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	AA_permease_2	85..1068	CDD:459263	292/1161 (25%)
slc12a4	XP_691291.2	2a30	50..1095	CDD:273347	298/1198 (25%)