Protein Alignment NKCC and slc12a10.3

DIOPT Version :9

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: XP_021333240.1
• Gene: slc12a10.3 / 562763
• ZFINID: ZDB-GENE-060503-518
• Length: 1001
• Species: Danio rerio
• Alignment Length: 1044
• Identity: 392/1044 - (37%)
• Similarity: 589/1044 - (56%)
• Gaps: 132/1044 - (12%)

  Fly    71 LRKSDAERKFSLAQLTKESLPRLDNY-------RISMRNLKRPSIGELQGEAVDQSITIPEPE-- 126
            :.:...:||.|:.. ..:..|:.:.|       ||..   .|||:..|:..        |:|:
Zfish    41 MEQEQDKRKMSIFN-PLDPTPKFEFYAKDTWTGRIKS---ARPSLDVLRKS--------PDPDDL 93

  Fly   127 ---PEATGGH----IKLGWIVGVLIPCLLNIWGVMLFLRLSWVVAESGILQSLIIITISAVVCVI 184
               ||..|..    ::.||::||:|.|:||||||::||||.|:.:::||:.:.:||.:|..:..|
Zfish    94 PPPPEDDGRPKVKLVRFGWVLGVMIRCMLNIWGVIMFLRLPWITSQAGIILTYVIIFMSVTITTI 158

  Fly   185 TTLSLSAISTNGEVKGGGVYFIISRSLGPEFGASVGVVFAFANAVSASMNTIGFCESLNVLLKNN 249
            |..|:|||||||:|..||.||:||||||||.||.:|::|:||||::.|:||:||.|::..:||.|
Zfish   159 TATSVSAISTNGKVYSGGTYFLISRSLGPELGAPIGLLFSFANALACSLNTVGFAETVRDVLKEN 223

  Fly   250 DLKIVDNGINDIRIVGSITVLVLILICCVGMEWETKAQNFLIVTIVLAIFNFLIGAAIGPQGNEE 314
            :.::||: :||:||:|:|||.:|:||...||.||.|||....:.:::::.|:.:|..:.|...::
Zfish   224 NAQMVDD-VNDVRIIGTITVCILLLITFAGMAWEAKAQILFFIALLISLANYFVGTLLPPTPEKQ 287

  Fly   315 QISRGFVGFSWATLKENFGSDYRYAEGVNHDFFSVFAIFFPSVTGIQAGANICGDLKDAGAAIPK 379
            .:  ||.|:......:|....:|..:|   .||.:|:|||||.|||.:|.||||||||....|||
Zfish   288 AV--GFFGYRAEIFVDNLLPSFRGKDG---SFFRMFSIFFPSATGILSGVNICGDLKDPSGGIPK 347

  Fly   380 GTFWSLLISMSSYALFVLFAGGAAVRDASGIPADLVNGTIVSSELPCMATG-------------- 430
            ||..::..:..||.|..:....:.||||||   :|.:...:.|.:.|...|
Zfish   348 GTLLAIFWTTVSYLLIAITVAASVVRDASG---NLNDSLALDSSIQCSGLGCKLGWNFDHCEQNH 409

  Fly   431 NCTWGLFNSYEMMQEMSLWGPLIYAGCFAATLSTALTNLLSVPRLVQALGIDQIYPGLIFFSKPY 495
            .|::||.|.::::...|..|.||..|.||||||:||..|:|.|::.|.|..|.|||.:.||.|.|
Zfish   410 TCSFGLANYFQILTTASGTGHLITIGIFAATLSSALGFLVSAPKIFQCLCKDNIYPYIGFFGKGY 474

  Fly   496 GKHGEPYRGYVLTFFITTGFLLIGELNLIAPLISTFYLASYALINFCTFHAAFVKPLGWRPTFKY 560
            ||:.||.|||:|.|||...|:|||:||.||||||.|:|.||.||||..|||...|..||||.:.|
Zfish   475 GKNQEPLRGYLLMFFIALAFILIGDLNTIAPLISNFFLCSYGLINFSCFHATITKSPGWRPHYHY 539

  Fly   561 YNAWLSLFGFAMCVAIMFLINYVAAIITFGIIFALYLVVMYRKPEANWGSTTQAQQYKAALMAVH 625
            ::.|.|||...:...:|||..:.||::||.|:.:|...|.|:||:.|||::.||..|..||....
Zfish   540 FSPWTSLFAAFLSFILMFLFTWWAALVTFAIVLSLLGYVTYKKPKINWGTSYQASFYNMALSFSM 604

  Fly   626 RLQNVSDHVKNYHPQVLVLSGDPKTRPPLVDFGYLLTKNNSLMFVANIIPVRVGYKNRQHLVKDG 690
            .|..|.|||||:.||.|||:|.|..||.||||....|||.|||...||..|....:..||.....
Zfish   605 SLTGVEDHVKNFRPQCLVLTGPPNVRPALVDFVGTFTKNISLMICGNIFMVEEKSRFPQHSTDML 669

  Fly   691 QKYLDARKIKAFYNVIDGFSLEDGINALTKSTGFGKMSPNIVLVGYKPDWNRCRKEEVESYFSIL 755
            ..:|:.||::|||......||.:|.::|.:::|.||:.||.:::|||.:|..|:.|.::.||:.:
Zfish   670 VDWLNQRKVRAFYTSFTADSLTEGTHSLMQASGLGKLKPNTLVLGYKTNWQECKPERLQDYFNTI 734

  Fly   756 YNAFSQRMGVALLRLPNGLDFSE-----LSSEVTLPANGMGHMHTANAHGFTNELMPAANAASEL 815
            .:||....|:|:||:.:|||..|     .||.:..||             |..:    .|..||.
Zfish   735 SDAFDSSYGIAVLRMMDGLDIREDLTSGRSSAIDNPA-------------FEAD----ENTKSEN 782

  Fly   816 LHIDSNLNLASMDSPNSSFTMPQPAPMPNMQRNSRSYKVTSSDEPAVTYHTKGGSDIPQNLLDAM 880
            ...|.|.::                                ||:        |.:|      ...
Zfish   783 EETDRNSDI--------------------------------SDD--------GSND------QVK 801

  Fly   881 TIFTRKQPKGTIDVFWLYDDGGLTILLPYIISMRSHWQNSKLRVFAMCHGKDEEQEEKSMASLLT 945
            |:|..||.:.|||::|:.||||||:|:||:::.|:.|:.||:|||.:...:..:::.|.|..||.
Zfish   802 TVFQTKQGRKTIDIYWISDDGGLTLLVPYLLTRRNRWKKSKIRVFVLGDQETMQEDHKDMKMLLK 866

  Fly   946 KFRIKYSELIMLKGVSEQPRADTVLKHKRLIEPFR------RGARNEF------GITDDELQSMS 998
            :||::..::|::..|.:.|.|..:.::...:.|||      ||...||      .::|.:|:::.
Zfish   867 RFRLEIEDIIVITDVDKPPLAKNLQRYDDSVAPFRLTEEQSRGDLQEFRRLNPWTVSDKDLEAIR 931

  Fly   999 EKTNRQLRIHELVVKHSSNASLVVMSLPMPRKEAISAPLYMSWLEMLTSDMKCPVALARGNQTPV 1063
            .|..|.:|::|::.|:|::|:|||:|||:|.....|: |||:|:|.|:..:.||..|.||||..|
Zfish   932 PKIERTVRLNEIIKKNSNHAALVVVSLPVPDLNCPSS-LYMAWMEALSFGIHCPALLIRGNQENV 995

  Fly  1064 LTLY 1067
            :|.|
Zfish   996 MTFY 999

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	2a30	85..1068	CDD:273347	389/1030 (38%)
		DUF502	507..>643	CDD:294696	67/135 (50%)
		SLC12	651..1068	CDD:281515	138/434 (32%)
slc12a10.3	XP_021333240.1	SLC12	54..1000	CDD:332077	389/1031 (38%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG2083
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	817	1.000	Inparanoid score	I458
OMA	1	1.010	-	-		QHG57166
OrthoDB	1	1.010	-	-		D135130at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm6412
orthoMCL	1	0.900	-	-		OOG6_101360
Panther	1	1.100	-	-	O	PTHR11827
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	9	8.840