Protein Alignment NKCC and slc12a7a

DIOPT Version :10

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: XP_005171306.1
• Gene: slc12a7a / 558214
• ZFINID: ZDB-GENE-070912-696
• Length: 1117
• Species: Danio rerio
• Alignment Length: 1290
• Identity: 308/1290 - (23%)
• Similarity: 496/1290 - (38%)
• Gaps: 395/1290 - (30%)

  Fly     1 MADRFQISKVNGT--ANAGYDGEA---RSAESDQTDAAHPLPPTSNENHLHPKASGETGENRRSS 60
            |...|.:..|..|  ::...||:.   ...|..:|.|.|           :|   |:......||
Zfish     1 MPTNFTVVPVEDTPLSDLDCDGDGLKEEDKEEPETAAGH-----------YP---GDETPRETSS 51

  Fly    61 RLSFRGFGNFLRKSDA--ERKFSLAQLTKESLPRLDNYRISMRNLKRPSIGELQGEAVD--QSIT 121
            .:|.....:...|:.|  |.:.....:....|.:|.||    .|:.:.::...:.|:.|  |.:|
Zfish    52 LISTDHDDSTYGKNMALYEEEMDSTPMVSSLLNKLANY----TNITQGAVEHEEAESEDGIQRVT 112

  Fly   122 IPEPEPEATGGHIKLGWIVGVLIPCLLNIWGVMLFLRLSWVVAESGILQSLIIITISAVVCVITT 186
            :..|:         :|..:||.:||..||.||:|||||:|:|..:|||::|.::.:.....::|.
Zfish   113 VNGPQ---------MGTFIGVFLPCTQNILGVILFLRLTWIVGTAGILEALAVVFMCCACTMLTA 168

  Fly   187 LSLSAISTNGEVKGGGVYFIISRSLGPEFGASVGVVFAFANAVSASMNTIGFCE--------SLN 243
            :|:|||:|||.|..||.|::|||:||||.|.:||:.|......:.||..:|..|        |..
Zfish   169 ISMSAIATNGVVPAGGSYYMISRALGPEIGGAVGLCFYLGTTFAGSMYILGTMEIFLTYIMPSAA 233

  Fly   244 VLLKNNDLKIVDNGI-NDIRIVGSITVLVLILICCVGMEWETK-AQNFL--IVTIVLAIFNFLIG 304
            |...||     ..|: |::||.|:..:.::.|:..||:::..| :..||  :|..::||:..:|.
Zfish   234 VFEANN-----PTGMQNNMRIYGTCCLAIMTLVVFVGVKYVNKLSLVFLSCVVLSIMAIYAGVIQ 293

  Fly   305 AAIGPQ-------GNE--------------------EQISR------------------------ 318
            :||.|.       ||.                    :.|::
Zfish   294 SAIKPSSFTICLLGNRTLQHFGKSGKFDKCMKTEVIDNITKPTELWYHFCNGTCDEYFNLNELTE 358

  Fly   319 --GFVGFSWATLKENFGSDY-RYAEGVNHD-------------------------FFSVF-AIFF 354
              ...|.....:.||..|.| .|...|..:                         ||::. .|:|
Zfish   359 ISAVPGLLSNAITENLWSRYGEYRTTVEKENITSEAALSSEPEVLNSYVVNDMATFFTLLVGIYF 423

  Fly   355 PSVTGIQAGANICGDLKDAGAAIPKGTFWSL----LISMSSYALFVLFAGGAAVRDASGIPADLV 415
            ||||||.||:|..|||:|...:||.||..::    .|.:|...||.....|..:||..|   |.|
Zfish   424 PSVTGIMAGSNRSGDLRDPQRSIPTGTIMAIATTSFIYISCVVLFGACFEGVVLRDKYG---DSV 485

  Fly   416 NGTIVSSELPCMATGNCTWGLFNSYEMMQEMSLWGPLIYAGCFAATLSTALTNLLSVPRLVQALG 480
            |..:|.:.|        .|           .|.|  :|..|.|.:.....|.:|...||::||:.
Zfish   486 NKNLVVATL--------AW-----------PSPW--VIVIGSFFSCCGAGLQSLTGAPRILQAIA 529

  Fly   481 IDQIYPGLIFFSKPYGKH---GEPYRGYVLTFFITTGFLLIGELNLIAPLISTFYLASYALINFC 542
            .|.|.|    |.:.:|:.   |||....:||..|....:||..|:.:||::|.|:|..|..:|..
Zfish   530 RDGIMP----FLQVFGRSKSGGEPTWALLLTAAICEIGILIASLDAVAPILSMFFLMCYLFVNLA 590

  Fly   543 TFHAAFVKPLGWRPTFKYYNAWLSLFGFAMCVAIMFLINYVAAIITFGIIFALYLVVMYRKPEAN 607
            ......::...|||.||:|:..||..|.::.:::||:.::..|::...|...:|..:.||.....
Zfish   591 CAVQTLLRTPNWRPRFKFYHWSLSFLGMSLSLSLMFVSSWYYALVVILIAGCIYKYIEYRGAVKE 655

  Fly   608 WGSTTQAQQYKAALMAVHRLQNVSDHVKNYHPQVLV---LSGDPKTR-PPLVDFGYLLTKNNSLM 668
            ||...:.....||..|:.:|:....|.||:.||:||   |..|...: |.|:.|...|.....|.
Zfish   656 WGDGLRGLSLNAARYALIKLEEAQPHTKNWRPQLLVLMKLDTDLTVKHPRLLSFTSQLKAGKGLT 720

  Fly   669 FVANIIPVRVGYKNRQHLVKDGQKYLDARKIKA---------FYNVIDGFSLEDGINALTKSTGF 724
            .|.:::       ....:|:.....|..:.|||         |.:|:...:..||.:.|.:|.|.
Zfish   721 IVCSVL-------EGTFMVRGADAKLAEQNIKAAMAKEKTKGFCHVVVSSNQRDGFSHLIQSAGL 778

  Fly   725 GKMSPNIVLVGYKPDWNRCRKEEVESYFSILYNAFSQRMGVALLRLPNGLDFSELSSEVTLPANG 789
            |.|..|.|||.:..:|     ::.||..|                   ..:|.|...|.|
Zfish   779 GGMKHNAVLVAWPANW-----KQAESSLS-------------------WKNFIETVRETT----- 814

  Fly   790 MGHMHTANAHGFTNELMPAANAASELLHIDSNLNLASMDSPNSSFTMPQPAPMPNMQRNSRSYKV 854
                                 ||.:.|.:..|::           |.|
Zfish   815 ---------------------AAHQALLVAKNVD-----------TFP----------------- 830

  Fly   855 TSSDEPAVTYHTKGGSDIPQNLLDAMTIFTRKQPKGTIDVFWLYDDGGLTILLPYIISMRSHWQN 919
            ::.|..|                           :|||||:|:..||||.:|||:::.....|:.
Zfish   831 SNQDRLA---------------------------EGTIDVWWIVHDGGLLMLLPFLLRQHKVWRK 868

  Fly   920 SKLRVFAMCHGKDEE-QEEKSMASLLTKFRI-----------------KYSE-LIM--------- 956
            .|:|:|.:....|.. |.:|.:...|...|:                 .|.: |:|
Zfish   869 CKMRIFTVAQMDDNSIQMKKDLQMFLYHLRLDAKVEVVEMNAGDISAFTYEKTLVMEQRSQMLKQ 933

  Fly   957 -----------LKGVSEQPRA-------------DTVLK--------------------HKRLIE 977
                       ::.:|::.|.             ::.||                    |..|:|
Zfish   934 MQLSKTEREREIQSISDESRISIRRKPCGEKQTHNSKLKVATVNLDEEAQLIHDKNTKSHATLVE 998

  Fly   978 PFRR---------------GARN-----EFGITD-----DELQSMSEKTNRQL----RIHELVVK 1013
            ....               |.||     ...:.|     .|.:::.:...|::    :::|:|||
Zfish   999 KATPTSDRVHMTWTKEKLIGERNRLRDASMHVRDIFNMKPEWENLDQTNVRRMHTAVKLNEVVVK 1063

  Fly  1014 HSSNASLVVMSLPMPRKEAISAPLYMSWLEMLTSDMKCPVALARGNQTPVLTLYS 1068
            .|..|.||::::|.|.|.......||.:||:||..:. .|.|.||....|:|:||
Zfish  1064 KSKGAQLVLLNMPGPPKNKGGDENYMEFLEVLTEGLD-HVLLVRGGGREVITIYS 1117

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	AA_permease_2	85..1068	CDD:459263	288/1197 (24%)
slc12a7a	XP_005171306.1	AA_permease_2	62..1117	CDD:459263	291/1213 (24%)