Protein Alignment NKCC and Slc12a3

DIOPT Version :10

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: XP_063134335.1
• Gene: Slc12a3 / 54300
• RGDID: 3686
• Length: 1028
• Species: Rattus norvegicus
• Alignment Length: 1087
• Identity: 416/1087 - (38%)
• Similarity: 605/1087 - (55%)
• Gaps: 147/1087 - (13%)

  Fly    37 PPTSNEN----HLHPKASGETGENRRSSRLSFRGFG-NFLRKSDAERKFSLAQLTKESLPRLDNY 96
            ||.:.:|    ||           ...|.|..|.|| |.:....|...::.:.|..|  ||  ..
  Rat    31 PPAACDNSQPSHL-----------THGSTLYLRTFGYNTIDVVPAYEHYANSALPGE--PR--KV 80

  Fly    97 RISMRNLK-----------------RPSIGELQGEAVDQSITIPEPEPEATGGHIKLGWIVGVLI 144
            |.::.:|.                 ||......|...|::....|..|   |..::.||:.||:|
  Rat    81 RPTLADLHSFLKQEGSHLHALAFDGRPGHELTDGLVEDETGANSEKSP---GEPVRFGWVKGVMI 142

  Fly   145 PCLLNIWGVMLFLRLSWVVAESGILQSLIIITISAVVCVITTLSLSAISTNGEVKGGGVYFIISR 209
            .|:||||||:|:|||.|:.|::||:.:.:||.:|.:|..||.||:|||||||:||.||.||:|||
  Rat   143 RCMLNIWGVILYLRLPWITAQAGIVLTWLIILLSVMVTSITGLSISAISTNGKVKSGGTYFLISR 207

  Fly   210 SLGPEFGASVGVVFAFANAVSASMNTIGFCESLNVLLKNNDLKIVDNGINDIRIVGSITVLVLIL 274
            |||||.|.|:|::|||||||..:|:|:||.|::..||:.|...||| .||||||:|.:||.||:.
  Rat   208 SLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQENGTPIVD-PINDIRIIGVVTVTVLLA 271

  Fly   275 ICCVGMEWETKAQNFLIVTIVLAIFNFLIGAAIGPQGNEEQISRGFVGFSWATLKENFGSDYRYA 339
            |...|||||:|||....:.|:::..|:|:|..|  ..::::.|:||..:......:|...|:|
  Rat   272 ISLAGMEWESKAQVLFFLVIMVSFANYLVGTLI--PASKDKASKGFYSYHGDIFVQNLVPDWR-- 332

  Fly   340 EGVNHDFFSVFAIFFPSVTGIQAGANICGDLKDAGAAIPKGTFWSLLISMSSYALFVLFAGGAAV 404
             |::..||.:|:|||||.|||.|||||.|||||...||||||..::..:..||.......|...|
  Rat   333 -GIDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTISYLAISATIGSCVV 396

  Fly   405 RDASGIPADLVNGTIVSSELPC--MATG------------NCTWGLFNSYEMMQEMSLWGPLIYA 455
            |||||.    ||.|:......|  :|.|            :|.:||.|.|:.|..:|.:.|||.|
  Rat   397 RDASGD----VNDTVTPGPGLCEGLACGYGWNFTECSQQHSCRYGLINYYQTMSMVSAFAPLITA 457

  Fly   456 GCFAATLSTALTNLLSVPRLVQALGIDQIYPGLIFFSKPYGKHGEPYRGYVLTFFITTGFLLIGE 520
            |.|.||||:||..|:|..::.|.|..||:||.:.||.|.|||:.||.|||:|.:.|...|::|.|
  Rat   458 GIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAE 522

  Fly   521 LNLIAPLISTFYLASYALINFCTFHAAFVKPLGWRPTFKYYNAWLSLFGFAMCVAIMFLINYVAA 585
            ||.|||:||.|:|.|||||||..|||:.....||||:|:||:.|.:|||..:.|.||||:.:.||
  Rat   523 LNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAVISVVIMFLLTWWAA 587

  Fly   586 IITFGIIFALYLVVMYRKPEANWGSTTQAQQYKAALMAVHRLQNVSDHVKNYHPQVLVLSGDPKT 650
            :|..|::..|.|.|:|:|||.||||:.||..|..||.....|..|.||:|||.||.|||:|.|..
  Rat   588 LIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNF 652

  Fly   651 RPPLVDFGYLLTKNNSLMFVANIIPVRVGYKNR---QHLVKDGQ-KYLDARKIKAFYNVIDGFSL 711
            ||.||||....|:|.|||...::: :..| |.|   ..|:..|. |:|:.|||||||:.:....|
  Rat   653 RPALVDFVSTFTQNLSLMICGHVL-IAPG-KQRVPELRLIASGHTKWLNKRKIKAFYSDVIAEDL 715

  Fly   712 EDGINALTKSTGFGKMSPNIVLVGYKPDWNRCRKEEVESYFSILYNAFSQRMGVALLRLPNGLDF 776
            ..|:..|.:::|.|:|.|||::||:|.:|.......:|.|..||::||....||.::|:..||:.
  Rat   716 RSGVQILMQASGLGRMKPNILVVGFKKNWQSAHPATLEDYIGILHDAFDFNYGVCIMRMREGLNV 780

  Fly   777 SELSSEVTLPANGMGHMHTANAHGFTNELMPAANAASELLHIDSNLNLASMDSPNSSFTMPQPAP 841
            ||.                                      :.:::|                 |
  Rat   781 SEA--------------------------------------LQTHIN-----------------P 790

  Fly   842 MPNMQRNSRSYKVTSSDEPAVTYHTKGGSDIPQNLL---DAMTIFTRKQPKGTIDVFWLYDDGGL 903
            |.:.:...|......:. |:|:     |...|:.|:   ...|||..:|.|.|||::||:|||||
  Rat   791 MFDPREGDRRASGRKAG-PSVS-----GMAAPEALVQEEQTSTIFQSEQGKKTIDIYWLFDDGGL 849

  Fly   904 TILLPYIISMRSHWQNSKLRVFAMCHGKDEEQEEKSMASLLTKFRIKYSELIMLKGVSEQPRADT 968
            |:|:||::..:..|...|:|||........::|.|::.|||:|||:.:.|:.:|..::::|:.:.
  Rat   850 TLLIPYLLHRKKRWGKCKIRVFVGGQINRMDEERKAIISLLSKFRLGFHEVHVLPDINQKPQVEH 914

  Fly   969 VLKHKRLIEPFR--RGARNE-----------FGITDDELQSMSEKTNRQLRIHELVVKHSSNASL 1020
            ..:.:.:|.|||  .|.::|           :.|:|:|:.....|:.||:|::|:::.:|.:|:|
  Rat   915 TKRFEDMIAPFRLNDGFKDEATVAEMRRDYPWKISDEEINKNRIKSLRQVRLNEILLDYSRDAAL 979

  Fly  1021 VVMSLPMPRKEAISAPLYMSWLEMLTSDMKCPVALARGNQTPVLTLY 1067
            ::::||:.||....:.|||:|||.|:.|:..||.|.||||..|||.|
  Rat   980 IILTLPIGRKGKCPSSLYMAWLETLSQDLSPPVLLIRGNQENVLTFY 1026

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	AA_permease_2	85..1068	CDD:459263	404/1034 (39%)
Slc12a3	XP_063134335.1	None