Protein Alignment NKCC and slc12a2

DIOPT Version :9

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: NP_001002080.1
• Gene: slc12a2 / 415170
• ZFINID: ZDB-GENE-040625-53
• Length: 1136
• Species: Danio rerio
• Alignment Length: 1136
• Identity: 443/1136 - (38%)
• Similarity: 657/1136 - (57%)
• Gaps: 150/1136 - (13%)

  Fly     4 RFQISKVNGTANAGYDGEARSAESDQTDAAHPLPPTSNENHLHPKASGETGE--NRRSSRLSFRG 66
            ||::  ||..|::.....|.:|::..|        ..:|..||....|:...  :..::....|.
Zfish    80 RFRV--VNFAASSPDAAPAETAQNGDT--------VMSEGSLHSSTGGQQHHHYDTHTNTYYLRT 134

  Fly    67 FGNFLRKSDAERKFSLAQLTKESLPRLDNYRISM----RNLKRPSIGELQGE----------AVD 117
            ||:               .|.:::|::|.||.:.    ..|.||::.||..|          |..
Zfish   135 FGH---------------NTIDAVPKIDFYRQTAAPLGEKLIRPTLSELHDELDKEPFEDGFANG 184

  Fly   118 QSITIPEPEPEA-----TGGHIKLGWIVGVLIPCLLNIWGVMLFLRLSWVVAESGILQSLIIITI 177
            :.:| |..|..|     :.|.:|.|||.|||:.|:|||||||||:|::|:|.::||..|.||:.:
Zfish   185 EELT-PAEESAAKDVSESKGVVKFGWIKGVLVRCMLNIWGVMLFIRMTWIVGQAGIAYSCIIVIM 248

  Fly   178 SAVVCVITTLSLSAISTNGEVKGGGVYFIISRSLGPEFGASVGVVFAFANAVSASMNTIGFCESL 242
            :.||..||..|.|||:|||.|:|||.|::||||||||||.|:|::|||||||:.:|..:||.|::
Zfish   249 ATVVTTITGCSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETV 313

  Fly   243 NVLLKNNDLKIVDNGINDIRIVGSITVLVLILICCVGMEWETKAQNFLIVTIVLAIFNFLIGAAI 307
            ..||.::.|.::|. .||||::|:|||::|:.|...|||||.|||.||:|.::.||||:.||:.|
Zfish   314 VELLMDSGLLMIDQ-TNDIRVIGTITVILLLGISVAGMEWEAKAQIFLLVILITAIFNYFIGSFI 377

  Fly   308 GPQGNEEQISRGFVGFSWATLKENFGSDYRYAEGVNHDFFSVFAIFFPSVTGIQAGANICGDLKD 372
            .....::   .||..:....|.||||.|:|     ...|||||:||||:.|||.|||||.|||.|
Zfish   378 AVDSKKK---FGFFSYDAGILAENFGPDFR-----GQTFFSVFSIFFPAATGILAGANISGDLAD 434

  Fly   373 AGAAIPKGTFWSLLISMSSYALFVLFAGGAAVRDASGIPADLVNGTIVS--SELPCM-------- 427
            ...||||||..::||:...|....:.||...||||:||.:   |.|::|  ::..|.
Zfish   435 PQMAIPKGTLLAILITGLVYVGVAISAGACIVRDATGIES---NFTLISNCTDAACKYGYDFSSC 496

  Fly   428 ------ATGNCTWGLFNSYEMMQEMSLWGPLIYAGCFAATLSTALTNLLSVPRLVQALGIDQIYP 486
                  ...:|.:||.|.:::|..:|.:.|||.||.|:||||:||.:|:|.|::.|||..|.|||
Zfish   497 RPTVEGEVSSCKFGLHNDFQVMSVVSGFSPLISAGIFSATLSSALASLVSAPKVFQALCKDNIYP 561

  Fly   487 GLIFFSKPYGKHGEPYRGYVLTFFITTGFLLIGELNLIAPLISTFYLASYALINFCTFHAAFVKP 551
            |:..|.|.|||:.||.|||.|||.|...|:||.|||:|||:||.|:|||||||||..|||:....
Zfish   562 GIAIFGKGYGKNNEPLRGYFLTFGIALAFILIAELNVIAPIISNFFLASYALINFSVFHASLANS 626

  Fly   552 LGWRPTFKYYNAWLSLFGFAMCVAIMFLINYVAAIITFGIIFALYLVVMYRKPEANWGSTTQAQQ 616
            .||||:|||||.|.||.|..:|..:||:||:.||::|..|:.:||:.|.|:||:.||||:|||..
Zfish   627 PGWRPSFKYYNMWASLAGAILCCVVMFIINWWAALLTNVIVLSLYIYVSYKKPDVNWGSSTQALT 691

  Fly   617 YKAALMAVHRLQ--NVSDHVKNYHPQVLVLSGDPKTRPPLVDFGYLLTKNNSLMFVANIIPVRVG 679
            |..||  .|.||  .|:||:|.:.||.||::|.|.:||.::...:..|||..||...:   ||:.
Zfish   692 YHQAL--THSLQLCGVADHIKTFRPQCLVMTGAPNSRPAILHLVHAFTKNVGLMLCGH---VRIS 751

  Fly   680 YK--NRQHLVKD---GQKYLDARKIKAFYNVIDGFSLEDGINALTKSTGFGKMSPNIVLVGYKPD 739
            .:  |.:.|..|   .|::|.....||||..:....|..|...:.::.|.|::.||.:::|:|.|
Zfish   752 SRRPNFKELNSDMLRYQRWLLNNNSKAFYTCVVAEDLRQGTQYMLQAAGLGRLRPNTLVIGFKND 816

  Fly   740 WNRCRKEEVESYFSILYNAFSQRMGVALLRLPNGLDFSELSSEVTLPANGMGHMHTANAHGFTNE 804
            |.....:|||:|.:::::||..:.||.:|||..|||.|.:..:.  .::||..:
Zfish   817 WRIGDIKEVETYINLIHDAFDFQYGVVILRLREGLDISHIQGQD--DSSGMKDV----------- 868

  Fly   805 LMPAANAASELLHIDSNLNLASMDSPNSSFTMPQPAPMPNMQRNSRSYKVTSSDEPAVTYHT--- 866
                      ::.:|     .|.||...|   .:|:......:||.:.:....|:...  ||
Zfish   869 ----------VVSVD-----ISKDSDGDS---SKPSSKATSVQNSPAVQKDEDDDGKA--HTQPL 913

  Fly   867 ----KGGSDIP-----QNLLDAMTIFTRKQPKGTIDVFWLYDDGGLTILLPYIISMRSHWQNSKL 922
                |....:|     |.||||...|.:||.|||:||:||:||||||:|:||:|:.:..|::.|:
Zfish   914 LKKDKKSPTVPLNVADQRLLDASQQFQQKQGKGTVDVWWLFDDGGLTLLIPYLIANKKKWKDCKI 978

  Fly   923 RVFAMCHGKDE--EQEEKSMASLLTKFRIKYSELIMLKGVSEQPRADTVLKHKRLIEPF------ 979
            |||  ..||..  :.:.::||:||:||||.:|::.:|..::.:|:::.:.:...:|||:
Zfish   979 RVF--IGGKINRIDHDRRAMATLLSKFRIDFSDITVLGDINTKPKSEGLTEFAEMIEPYKLREDD 1041

  Fly   980 -------RRGARNEFGITDDELQSMSEKTNRQLRIHELVVKHSSNASLVVMSLPMPRKEAISAPL 1037
                   :..:...:.|||:||:....|.|||:|::||:.:|||.|:|:|||:|:.||.|:|:.|
Zfish  1042 MEQEAAEKLKSEEPWRITDNELELYKAKGNRQIRLNELLKEHSSTANLIVMSMPLARKGAVSSAL 1106

  Fly  1038 YMSWLEMLTSDMKCPVALARGNQTPVLTLYS 1068
            ||:||:.|:.|:. |:.|.|||...|||.||
Zfish  1107 YMAWLDTLSKDLP-PILLVRGNHQSVLTFYS 1136

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	2a30	85..1068	CDD:273347	426/1051 (41%)
		DUF502	507..>643	CDD:294696	75/137 (55%)
		SLC12	651..1068	CDD:281515	144/448 (32%)
slc12a2	NP_001002080.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..73
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	91..121		7/37 (19%)
		AA_permease_N	124..185	CDD:285588	15/75 (20%)
		2a30	138..1136	CDD:273347	426/1051 (41%)
		Intg_mem_TP0381	<573..677	CDD:295259	58/103 (56%)
		SLC12	726..1136	CDD:281515	144/448 (32%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	875..921		11/50 (22%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	227	1.000	Domainoid score	I2442
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	817	1.000	Inparanoid score	I458
OMA	1	1.010	-	-		QHG57166
OrthoDB	1	1.010	-	-		D135130at33208
OrthoFinder	1	1.000	-	-		FOG0001536
OrthoInspector	1	1.000	-	-		mtm6412
orthoMCL	1	0.900	-	-		OOG6_101360
Panther	1	1.100	-	-	O	PTHR11827
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	10	9.980