Protein Alignment NKCC and Slc12a4

DIOPT Version :10

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: NP_062102.1
• Gene: Slc12a4 / 29501
• RGDID: 3687
• Length: 1085
• Species: Rattus norvegicus
• Alignment Length: 1259
• Identity: 305/1259 - (24%)
• Similarity: 486/1259 - (38%)
• Gaps: 372/1259 - (29%)

  Fly     5 FQISKVNGTANAGYD----------GEARSAESDQTDAAHPLPPTSNENHLHPKASGETGENRRS 59
            |.:..|:|.....||          ||  .||.:.:|.                    .|.:|.:
  Rat     4 FTVVPVDGPRRGDYDNLEGLSWVDYGE--RAEREDSDG--------------------QGNHREN 46

  Fly    60 SRLSFRGFGNFLRKSDAER-----KFSLAQLTKESLPRLDNYRISMRNLKRPSIGELQGEAVD-- 117
            |        .||...||.|     ..:||...:|    ||         .||.:..|.|:.|.
  Rat    47 S--------PFLSPLDASRGNDYYDRNLALFEEE----LD---------IRPKVSSLLGKLVSYT 90

  Fly   118 ---QSITIPEPEPEATGGHIK------LGWIVGVLIPCLLNIWGVMLFLRLSWVVAESGILQSLI 173
               |.....|......||..:      :|.::||.:|||.||:||:|||||:|:|..:|:||:|:
  Rat    91 NLTQGAKEHEEAESGEGGRRRAAKAPSMGTLMGVYLPCLQNIFGVILFLRLTWMVGTAGVLQALL 155

  Fly   174 IITISAVVCVITTLSLSAISTNGEVKGGGVYFIISRSLGPEFGASVGVVFAFANAVSASMNTIGF 238
            |:.|.....::|.:|:|||:|||.|..||.||:||||||||||.:||:.|......:|:|..:|.
  Rat   156 IVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAAAMYILGA 220

  Fly   239 CESLNVLLKNNDLKIVDNG--------INDIRIVGSITVLVLILICCVGMEWETKAQNFLIVTIV 295
            .|.|...:.........:|        :|::|:.|:|.:..:.|:..||:::..|..:..:..::
  Rat   221 IEILLTYIAPPAAIFYPSGTHDMSSATLNNMRVYGTIFLTFMTLVVFVGVKYVNKFASLFLACVI 285

  Fly   296 LAIFNFLIG---AAIGPQ--------------------------GNEEQISR------------- 318
            ::|.:..:|   :|..|.                          .||...:|
  Rat   286 ISILSIYVGGIKSAFDPPVFPVCMLGNRTLSRDQFDICAKTVVVDNETVATRLWTFFCHSPNLTA 350

  Fly   319 ----------------GFVGFSWATLKENFGSDY--------------------------RYAEG 341
                            |..|.:...|:||..|.|                          .....
  Rat   351 DSCDPYFLLNNVTEIPGIPGAAAGVLQENLWSAYLEKGEVVEKHGLPSTDTLGLKESLSLYVVAD 415

  Fly   342 VNHDFFSVFAIFFPSVTGIQAGANICGDLKDAGAAIPKGTFWSL----LISMSSYALFVLFAGGA 402
            :...|..:..|||||||||.||:|..|||:||..:||.||..::    |:..||..||.....|.
  Rat   416 IATSFTVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTTSLVYFSSVILFGACIEGV 480

  Fly   403 AVRD--ASGIPADLVNGTIVSSELPCMATGNCTWGLFNSYEMMQEMSLWGPLIYAGCFAATLSTA 465
            .:||  ..|:..:||.||:             .|           .|.|  :|..|.|.:|....
  Rat   481 VLRDKYGDGVSRNLVVGTL-------------AW-----------PSPW--VIVVGSFFSTCGAG 519

  Fly   466 LTNLLSVPRLVQALGIDQIYPGLIFFSKPYGK-HGEPYRGYVLTFFITTGFLLIGELNLIAPLIS 529
            |.:|...|||:||:..|.|.|.|..|.  :|| :|||....:||..|....:||..|:::||::|
  Rat   520 LQSLTGAPRLLQAIAKDNIIPFLRVFG--HGKANGEPTWALLLTALIAELGILIASLDMVAPILS 582

  Fly   530 TFYLASYALINFCTFHAAFVKPLGWRPTFKYYNAWLSLFGFAMCVAIMFLINYVAAIITFGIIFA 594
            .|:|..|..:|........::...|||.||||:..||..|.::|:|:||:.::..|::...|...
  Rat   583 MFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWALSFLGMSLCLALMFVSSWYYALVAMVIAGM 647

  Fly   595 LYLVVMYRKPEANWGSTTQAQQYKAALMAVHRLQNVSDHVKNYHPQVLV---LSGDPKTR-PPLV 655
            :|..:.|:..|..||...:.....||..|:.||:....|.||:.||:||   |..|...: |.|:
  Rat   648 IYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKYPRLL 712

  Fly   656 DFGYLLTKNNSLMFVANII--PVRVGYKNRQHLVKDGQKYLDARKIKAFYNVIDGFSLEDGINAL 718
            .|...|.....|..|.::|  .....|...|...:..:..::..|:|.|..|:....:.:|:..|
  Rat   713 TFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMEIEKVKGFCQVVVASKVREGLAHL 777

  Fly   719 TKSTGFGKMSPNIVLVGYKPDWNRCRKEEVESYFSILYNAFSQRMGVALLRLPNGLDFSELSSEV 783
            .:|.|.|.|..|.|::|:...|.:.........|            :..:|.
  Rat   778 IQSCGLGGMRHNSVVLGWPYGWRQSEDPRAWKTF------------IDTVRC------------- 817

  Fly   784 TLPANGMGHMHTANAHGFTNELMPAANAASELLHIDSNLNLASMDSPNSSFTMPQPAPMPNMQRN 848
                       |..||                        ||.:...|.:|
  Rat   818 -----------TTAAH------------------------LALLVPKNIAF-------------- 833

  Fly   849 SRSYKVTSSDEPAVTYHTKGGSDIPQNLLDAMTIFTRKQPKGTIDVFWLYDDGGLTILLPYIISM 913
                                   .|.|        ..:..:|.|||:|:..|||:.:|||:::..
  Rat   834 -----------------------YPSN--------HERYLEGHIDVWWIVHDGGMLMLLPFLLRQ 867

  Fly   914 RSHWQNSKLRVFAMCHGKDEE-QEEKSMASLLTKFRIK-----------------YSELIMLKGV 960
            ...|:..::|:|.:....|.. |.:|.:|..|...|::                 |...:|::..
  Rat   868 HKVWKKCRMRIFTVAQMDDNSIQMKKDLAIFLYHLRLEAEVEVVEMHNSDISAYTYERTLMMEQR 932

  Fly   961 SEQPRADTVLKHKR-----LIEPFRRGARNEFGITDDELQSMS---------------------- 998
            |:..|...:.|.:|     |::......|.|...:|:|.:|::
  Rat   933 SQMLRQMRLTKTERDREAQLVKDRHSALRLESLYSDEEDESVTGADKIQMTWTRDKYMAEPWDPS 997

  Fly   999 -------------------EKTNRQLRIHELVVKHSSNASLVVMSLPMPRKEAISAPLYMSWLEM 1044
                               .:.:..::::|::|..|.:|.||::::|.|.|.:.....||.:||:
  Rat   998 HAPDNFRELVHIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPKNSEGDENYMEFLEV 1062

  Fly  1045 LTSDMKCPVALARGNQTPVLTLYS 1068
            ||..:: .|.|.||....|:|:||
  Rat  1063 LTEGLE-RVLLVRGGGREVITIYS 1085

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	AA_permease_2	85..1068	CDD:459263	283/1162 (24%)
Slc12a4	NP_062102.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	32..56		9/51 (18%)
		2a30	42..1085	CDD:273347	292/1197 (24%)
		Scissor helix. /evidence=ECO:0000250|UniProtKB:Q9UP95	665..681		4/15 (27%)