DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment NKCC and Slc12a1

DIOPT Version :9

Sequence 1:	NP_730938.1	Gene:	NKCC / 40663	FlyBaseID:	FBgn0051547	Length:	1068	Species:	Drosophila melanogaster
Sequence 2:	NP_001257546.1	Gene:	Slc12a1 / 25065	RGDID:	3685	Length:	1095	Species:	Rattus norvegicus

Alignment Length:	1149	Identity:	454/1149 - (39%)
Similarity:	662/1149 - (57%)	Gaps:	149/1149 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 ADRFQISKVNGTANAGYDGEARSAESDQTDAAHPLPPTSNENHLHPKASGETGENRRSSRLSFRG 66
            |.|||:..:|....:|      :|.||.||     ||     |....:.|:..:||  .::|||.
  Rat    14 ASRFQVHVINEGHGSG------AAMSDSTD-----PP-----HYEETSFGDEAQNR--LKISFRP 60

  Fly    67 -----FGNFLRKSDAERK----------------FSLAQLTKESLPRLDNYR----ISMRNLKRP 106
                 :.|||:..:..:.                .:....|.:::|:::.||    :|...:.||
  Rat    61 GNQECYENFLQTGETAKTDTTFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSVSGPKVNRP 125

  Fly   107 SIGELQGEAVDQSITIP------------------EPEPEATGGHIKLGWIVGVLIPCLLNIWGV 153
            |:.|:..:........|                  |.:.|...|.:|.||:.|||:.|:||||||
  Rat   126 SLQEIHEQLAKNVAVAPGSADRVANGDGMPGDEQAENKEEDVTGVVKFGWVKGVLVRCMLNIWGV 190

  Fly   154 MLFLRLSWVVAESGILQSLIIITISAVVCVITTLSLSAISTNGEVKGGGVYFIISRSLGPEFGAS 218
            |||:||||:|.|:||...::||.:|.:|..||.||.|||:|||.|:|||.|::||||||||||.|
  Rat   191 MLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGS 255

  Fly   219 VGVVFAFANAVSASMNTIGFCESLNVLLKNNDLKIVDNGINDIRIVGSITVLVLILICCVGMEWE 283
            :|::|||||||:.:|..:||.|::..|||.:|..:|| ..|||||:|||||::|:.|...|||||
  Rat   256 IGLIFAFANAVAVAMYVVGFAETVVDLLKESDSMMVD-PTNDIRIIGSITVVILLGISVAGMEWE 319

  Fly   284 TKAQNFLIVTIVLAIFNFLIGAAIGPQGNEEQISRGFVGFSWATLKENFGSDYRYAEGVNHDFFS 348
            .|||..|:|.:::||.||.||..| |..||:: ||||..:..:...||||..:...||    |||
  Rat   320 AKAQVILLVILLIAIANFFIGTVI-PSNNEKK-SRGFFNYQASIFAENFGPSFTEGEG----FFS 378

  Fly   349 VFAIFFPSVTGIQAGANICGDLKDAGAAIPKGTFWSLLISMSSYALFVLFAGGAAVRDASGIPAD 413
            |||||||:.|||.|||||.|||:|...|||:||..::.|:..:|....:......||||:|
  Rat   379 VFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATG---- 439

  Fly   414 LVNGTIVSSELPCMATG--------------NCTWGLFNSYEMMQEMSLWGPLIYAGCFAATLST 464
            .:|.|:||. :.|..:.              .|.:||.|::::|..:|.:||||.||.|:||||:
  Rat   440 SMNDTVVSG-MNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSS 503

  Fly   465 ALTNLLSVPRLVQALGIDQIYPGLIFFSKPYGKHGEPYRGYVLTFFITTGFLLIGELNLIAPLIS 529
            ||.:|:|.|::.|||..|.|:.||.||:|.|||:.||.|||.|||.|...|:||.|||:|||:||
  Rat   504 ALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIIS 568

  Fly   530 TFYLASYALINFCTFHAAFVKPLGWRPTFKYYNAWLSLFGFAMCVAIMFLINYVAAIITFGIIFA 594
            .|:|||||||||..|||::.|..||||.:..||.|:||||..:|.|:||:||:.||:||:.|...
  Rat   569 NFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELF 633

  Fly   595 LYLVVMYRKPEANWGSTTQAQQYKAALMAVHRLQNVSDHVKNYHPQVLVLSGDPKTRPPLVDFGY 659
            ||:.|.|:||:.||||:|||..|.:||.....|..|.|||||:.||.:||:|.|.|||.|:|..:
  Rat   634 LYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITH 698

  Fly   660 LLTKNNSLMFVANII--PVRVGYKNRQHLVKDGQKYLDARKIKAFYNVIDGFSLEDGINALTKST 722
            ..|||:.|.....:.  |.::..|.....:...|.:|...||||||..:......||:.:|.:::
  Rat   699 AFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLMKNKIKAFYAAVAADCFRDGVRSLLQAS 763

  Fly   723 GFGKMSPNIVLVGYKPDWNRCRKEEVESYFSILYNAFSQRMGVALLRLPNGLDFS---ELSSEVT 784
            |.|:|.||.:::|||.:|.:....|:|:|..|:::||...:||.::|:..|.|.|   ::..|:
  Rat   764 GLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDEL- 827

  Fly   785 LPANGMGHMHTANAHGFTNELMPAANAASELLHIDSNL-----NLASMDSPNSSFTMPQPAPMPN 844
                               |.:.....|.|....|::.     .:..:........:.:|||
  Rat   828 -------------------EKLEQERLALEAAIKDNDCEEGKGGIRGLFKKAGKLNITKPAP--- 870

  Fly   845 MQRNSRSYKVTSSDEPAVTYHTKGGSDIPQNLLDAMTIFTRKQPKGTIDVFWLYDDGGLTILLPY 909
                       ..|....|..:....:..|.|::|...|.:||.||||||:||:||||||:|:||
  Rat   871 -----------KKDSNISTIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPY 924

  Fly   910 IISMRSHWQNSKLRVFAMCHGKDE--EQEEKSMASLLTKFRIKYSELIMLKGVSEQPRADTVLKH 972
            |:::|..|::.|||::.  .||..  |:|:.||||||:|||||::::.::..::.:|..::....
  Rat   925 ILTLRKKWKDCKLRIYV--GGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVF 987

  Fly   973 KRLIEPFRRGARNE-------------FGITDDELQSMSEKTNRQLRIHELVVKHSSNASLVVMS 1024
            :.:|||:|....::             :.|||.||:::.||:.||:|::||:.:||..|:|:|:|
  Rat   988 EEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLS 1052

  Fly  1025 LPMPRKEAISAPLYMSWLEMLTSDMKCPVALARGNQTPVLTLYS 1068
            ||:.||.:||..|||:|||:||.::. ||.|.|||...|||.||
  Rat  1053 LPVARKGSISDLLYMAWLEILTKNLP-PVLLVRGNHKNVLTFYS 1095

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	2a30	85..1068	CDD:273347	429/1043 (41%)
		DUF502	507..>643	CDD:294696	76/135 (56%)
		SLC12	651..1068	CDD:281515	142/441 (32%)
Slc12a1	NP_001257546.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	26..45		9/34 (26%)
		AA_permease_N	86..152	CDD:285588	10/65 (15%)
		2a30	100..1095	CDD:273347	429/1043 (41%)
		SLC12	690..1095	CDD:281515	142/441 (32%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG2083
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG57166
OrthoDB	1	1.010	-	-		D135130at33208
OrthoFinder	1	1.000	-	-		FOG0001536
OrthoInspector	1	1.000	-	-		mtm8955
orthoMCL	1	0.900	-	-		OOG6_101360
Panther	1	1.100	-	-	O	PTHR11827
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	11	10.790