Protein Alignment NKCC and Slc12a4

DIOPT Version :10

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: NP_001240733.1
• Gene: Slc12a4 / 20498
• MGIID: 1309465
• Length: 1087
• Species: Mus musculus
• Alignment Length: 1259
• Identity: 304/1259 - (24%)
• Similarity: 487/1259 - (38%)
• Gaps: 370/1259 - (29%)

  Fly     5 FQISKVNGTANAGYD----------GEARSAESDQTDAAHPLPPTSNENHLHPKASGETGENRRS 59
            |.:..|:|.....||          ||  .||.:.:|                   ||...|.|.
Mouse     4 FTVVPVDGPRRGDYDNLEGLSWVDYGE--RAEREDSD-------------------GEGQGNHRE 47

  Fly    60 SRLSFRGFGNFLRKSDAER-----KFSLAQLTKESLPRLDNYRISMRNLKRPSIGELQGEAVD-- 117
            :       ..||...||.|     ..:||...:|    ||         .||.:..|.|:.|.
Mouse    48 N-------SPFLCPLDASRGNDYYDRNLALFEEE----LD---------IRPKVSSLLGKLVSYT 92

  Fly   118 ---QSITIPEPEPEATGGHIK------LGWIVGVLIPCLLNIWGVMLFLRLSWVVAESGILQSLI 173
               |.....|......||..:      :|.::||.:|||.||:||:|||||:|:|..:|:||:|:
Mouse    93 NLTQGAKEHEEAESGEGGRRRAAKAPSMGTLMGVYLPCLQNIFGVILFLRLTWMVGTAGVLQALL 157

  Fly   174 IITISAVVCVITTLSLSAISTNGEVKGGGVYFIISRSLGPEFGASVGVVFAFANAVSASMNTIGF 238
            |:.|.....::|.:|:|||:|||.|..||.||:||||||||||.:||:.|......:|:|..:|.
Mouse   158 IVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAAAMYILGA 222

  Fly   239 CESLNVLLKNNDLKIVDNG--------INDIRIVGSITVLVLILICCVGMEWETKAQNFLIVTIV 295
            .|.|...:.........:|        :|::|:.|:|.:.::.|:..||:::..|..:..:..::
Mouse   223 IEILLTYIAPPAAIFYPSGTHDMSSATLNNMRVYGTIFLTLMTLVVFVGVKYVNKFASLFLACVI 287

  Fly   296 LAIFNFLIG-------------AAIGPQ----------------GNEEQISR------------- 318
            ::|.:...|             ..:|.:                .||...:|
Mouse   288 ISILSIYAGGIKSIFDPPVFPVCMLGNRTLSRDQFDICAKTVVVDNETVATRLWTFFCHSPNLTA 352

  Fly   319 ----------------GFVGFSWATLKENFGSDY--------------------------RYAEG 341
                            |..|.:...|:||..|.|                          .....
Mouse   353 DSCDPYFLLNNVTEIPGIPGAAAGVLQENLWSAYLEKGEVVEKHGLPSTDTLGLKESLSLYVVAD 417

  Fly   342 VNHDFFSVFAIFFPSVTGIQAGANICGDLKDAGAAIPKGTFWSL----LISMSSYALFVLFAGGA 402
            :...|..:..|||||||||.||:|..|||:||..:||.||..::    |:..||..||.....|.
Mouse   418 IATSFTVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTTSLVYFSSVILFGACIEGV 482

  Fly   403 AVRD--ASGIPADLVNGTIVSSELPCMATGNCTWGLFNSYEMMQEMSLWGPLIYAGCFAATLSTA 465
            .:||  ..|:..:||.||:             .|           .|.|  :|..|.|.:|....
Mouse   483 VLRDKYGDGVSRNLVVGTL-------------AW-----------PSPW--VIVVGSFFSTCGAG 521

  Fly   466 LTNLLSVPRLVQALGIDQIYPGLIFFSKPYGK-HGEPYRGYVLTFFITTGFLLIGELNLIAPLIS 529
            |.:|...|||:||:..|.|.|.|..|.  :|| :|||....:||..|....:||..|:::||::|
Mouse   522 LQSLTGAPRLLQAIAKDNIIPFLRVFG--HGKANGEPTWALLLTALIAELGILIASLDMVAPILS 584

  Fly   530 TFYLASYALINFCTFHAAFVKPLGWRPTFKYYNAWLSLFGFAMCVAIMFLINYVAAIITFGIIFA 594
            .|:|..|..:|........::...|||.||||:..||..|.::|:|:||:.::..|::...|...
Mouse   585 MFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLALMFVSSWYYALVAMLIAGM 649

  Fly   595 LYLVVMYRKPEANWGSTTQAQQYKAALMAVHRLQNVSDHVKNYHPQVLV---LSGDPKTR-PPLV 655
            :|..:.|:..|..||...:.....||..|:.||:....|.||:.||:||   |..|...: |.|:
Mouse   650 IYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKYPRLL 714

  Fly   656 DFGYLLTKNNSLMFVANII--PVRVGYKNRQHLVKDGQKYLDARKIKAFYNVIDGFSLEDGINAL 718
            .|...|.....|..|.::|  .....|...|...:..:..:|..|:|.|..|:....:.:|:..|
Mouse   715 TFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMDIEKVKGFCQVVVASKVREGLAHL 779

  Fly   719 TKSTGFGKMSPNIVLVGYKPDWNRCRKEEVESYFSILYNAFSQRMGVALLRLPNGLDFSELSSEV 783
            .:|.|.|.|..|.|::|:...|.  :.|:..::.:.:............|.:|..:.|
Mouse   780 IQSCGLGGMRHNSVVLGWPYGWR--QSEDPRAWKTFIDTVRCTTAAHLALLVPKNIAF------- 835

  Fly   784 TLPANGMGHMHTANAHGFTNELMPAANAASELLHIDSNLNLASMDSPNSSFTMPQPAPMPNMQRN 848
             .|:|
Mouse   836 -YPSN------------------------------------------------------------ 839

  Fly   849 SRSYKVTSSDEPAVTYHTKGGSDIPQNLLDAMTIFTRKQPKGTIDVFWLYDDGGLTILLPYIISM 913
                            |        :..||           |.|||:|:..|||:.:|||:::..
Mouse   840 ----------------H--------ERYLD-----------GHIDVWWIVHDGGMLMLLPFLLRQ 869

  Fly   914 RSHWQNSKLRVFAMCHGKDEE-QEEKSMASLLTKFRIK-----------------YSELIMLKGV 960
            ...|:..::|:|.:....|.. |.:|.:|..|...|::                 |...:|::..
Mouse   870 HKVWKKCRMRIFTVAQMDDNSIQMKKDLAIFLYHLRLEAEVEVVEMHNSDISAYTYERTLMMEQR 934

  Fly   961 SEQPRADTVLKHKR-----LIEPFRRGARNEFGITDDELQSMS---------------------- 998
            |:..|...:.|.:|     |::......|.|...:|:|.:|::
Mouse   935 SQMLRQMRLTKTERDREAQLVKDRHSALRLESLYSDEEEESVAGADKIQMTWTRDKYMAEPWDPS 999

  Fly   999 -------------------EKTNRQLRIHELVVKHSSNASLVVMSLPMPRKEAISAPLYMSWLEM 1044
                               .:.:..::::|::|..|.:|.||::::|.|.|.:.....||.:||:
Mouse  1000 HAPDNFRELVHIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPKNSEGDENYMEFLEV 1064

  Fly  1045 LTSDMKCPVALARGNQTPVLTLYS 1068
            ||..:: .|.|.||....|:|:||
Mouse  1065 LTEGLE-RVLLVRGGGREVITIYS 1087

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	AA_permease_2	85..1068	CDD:459263	281/1162 (24%)
Slc12a4	NP_001240733.1	2a30	44..1087	CDD:273347	290/1196 (24%)
		Scissor helix. /evidence=ECO:0000250|UniProtKB:Q9UP95	667..683		4/15 (27%)