Protein Alignment NKCC and Slc12a3

DIOPT Version :9

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: XP_017168114.1
• Gene: Slc12a3 / 20497
• MGIID: 108114
• Length: 1009
• Species: Mus musculus
• Alignment Length: 1103
• Identity: 414/1103 - (37%)
• Similarity: 597/1103 - (54%)
• Gaps: 198/1103 - (17%)

  Fly    37 PP----TSNENHLHPKASGETGENRRSSRLSFRGFG-NFLRKSDAERKFSLAQLTKESLPRLDNY 96
            ||    :|..:||           ...|.|..|.|| |.:....|...::.:.|..|  ||
Mouse    31 PPAACDSSQPSHL-----------THGSTLYMRTFGYNTIDVVPAYEHYANSALPGE--PR---- 78

  Fly    97 RISMRNLKRPSIGEL-------------------QGEAV------DQSITIPEPEPEATGGHIKL 136
                  ..||::.:|                   ||..:      |::.|..|..|   |..::.
Mouse    79 ------KVRPTLADLHSFLKQEGSHLHALAFDGRQGRELTDGLVEDETGTNSEKSP---GEPVRF 134

  Fly   137 GWIVGVLIPCLLNIWGVMLFLRLSWVVAESGILQSLIIITISAVVCVITTLSLSAISTNGEVKGG 201
            ||:.||:|.|:||||||:|:|||.|:.|::||:.:.:||.:|.:|..||.||:|||||||:||.|
Mouse   135 GWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWLIILLSVMVTSITGLSISAISTNGKVKSG 199

  Fly   202 GVYFIISRSLGPEFGASVGVVFAFANAVSASMNTIGFCESLNVLL-------KNNDLKIVDNGIN 259
            |.||:||||||||.|.|:|::|||||||..:|:|:||.|::..||       :.....||| .||
Mouse   200 GTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQLACSPTQEYGTPIVD-PIN 263

  Fly   260 DIRIVGSITVLVLILICCVGMEWETKAQNFLIVTIVLAIFNFLIGAAIGPQGNEEQISRGFVGFS 324
            ||||:|.:||.||:.|...|||||:|||....:.|:::..|:|:|..|  ..:|::.|:||..:.
Mouse   264 DIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLI--PASEDKASKGFYSYH 326

  Fly   325 WATLKENFGSDYRYAEGVNHDFFSVFAIFFPSVTGIQAGANICGDLKDAGAAIPKGTFWSLLISM 389
            .....:|...|:|   |::..||.:|:|||||.|||.|||||.|||||...||||||..::..:.
Mouse   327 GDIFVQNLVPDWR---GIDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTT 388

  Fly   390 SSYALFVLFAGGAAVRDASGIPADLVNGTIVSSELPC--MATG------------NCTWGLFNSY 440
            .||.......|...||||||.    ||.|:.....||  :|.|            :|.:||.|.|
Mouse   389 ISYLAISATIGSCVVRDASGD----VNDTMTPGPGPCEGLACGYGWNFTECSQQRSCRYGLINYY 449

  Fly   441 EMMQEMSLWGPLIYAGCFAATLSTALTNLLSVPRLVQALGIDQIYPGLIFFSKPYGKHGEPYRGY 505
            :.|..:|.:.|||.||.|.||||:||..|:|..::.|.|..||:||.:.||.|.|||:.||.|||
Mouse   450 QTMSMVSAFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNREPVRGY 514

  Fly   506 VLTFFITTGFLLIGELNLIAPLISTFYLASYALINFCTFHAAFVKPLGWRPTFKYYNAWLSLFGF 570
            :|.:.|...|::|.|||.|||:||.|:|.|||||||..|||:.....||||:|:||:.|.:|||.
Mouse   515 LLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGA 579

  Fly   571 AMCVAIMFLINYVAAIITFGIIFALYLVVMYRKPEANWGSTTQAQQYKAALMAVHRLQNVSDHVK 635
            .:.|.||||:.:.||:|..|::..|.|.|:|:|||.||||:.||..|..||.....|..|.||:|
Mouse   580 VISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIK 644

  Fly   636 NYHPQVLVLSGDPKTRPPLVDFGYLLTKNNSLMFVANIIPVRVGYKNRQ----HLVKDGQ-KYLD 695
            ||.||.|||:|.|..||.||||....|:|.|||...:::   :|...::    .|:..|. |:|:
Mouse   645 NYRPQCLVLTGPPNFRPALVDFVSTFTQNLSLMICGHVL---IGPGKQRVPELRLIASGHTKWLN 706

  Fly   696 ARKIKAFYNVIDGFSLEDGINALTKSTGFGKMSPNIVLVGYKPDWNRCRKEEVESYFSILYNAFS 760
            .|||||||:.:....|..|:..|.:::|.|:|.|||::||:|.:|.......||.|..:|::||.
Mouse   707 KRKIKAFYSDVIAEDLRSGVQILMQASGLGRMKPNILVVGFKRNWQSAHPATVEDYIGVLHDAFD 771

  Fly   761 QRMGVALLRLPNGLDFSELSSEVTLPANGMGHMHTANAHGFTNELMPAANAASELLHIDSNLNLA 825
            ...||.::|:..||:.||
Mouse   772 FNYGVCVMRMREGLNVSE----------------------------------------------- 789

  Fly   826 SMDSPNSSFTMPQPAPMPNMQRNSRSYKVTSSDEPAVTYHTKGGSDIPQNLL---DAMTIFTRKQ 887
                                               |:..||     .|:.|:   .|.|||..:|
Mouse   790 -----------------------------------ALQTHT-----TPEALIQEEQASTIFQSEQ 814

  Fly   888 PKGTIDVFWLYDDGGLTILLPYIISMRSHWQNSKLRVFAMCHGKDEEQEEKSMASLLTKFRIKYS 952
            .|.|||::||:||||||:|:||::..:..|...|:|||........::|.|::.|||:|||:.:.
Mouse   815 GKKTIDIYWLFDDGGLTLLIPYLLHRKKRWGKCKIRVFVGGQINRMDEERKAIISLLSKFRLGFH 879

  Fly   953 ELIMLKGVSEQPRADTVLKHKRLIEPFR--RGARNE-----------FGITDDELQSMSEKTNRQ 1004
            |:.:|..::::|:|:...:.:.:|.|||  .|.::|           :.|:|:|:.....|:.||
Mouse   880 EVHVLPDINQKPQAEHTKRFEDMIAPFRLNDGFKDEATVTEMRRDCPWKISDEEINKNRIKSLRQ 944

  Fly  1005 LRIHELVVKHSSNASLVVMSLPMPRKEAISAPLYMSWLEMLTSDMKCPVALARGNQTPVLTLY 1067
            :|:.|:::.:|.:|:|::::||:.||....:.|||:|||.|:.|::.||.|.||||..|||.|
Mouse   945 VRLSEILLDYSRDAALIILTLPIGRKGKCPSSLYMAWLETLSQDLRPPVLLIRGNQENVLTFY 1007

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	2a30	85..1068	CDD:273347	402/1050 (38%)
		DUF502	507..>643	CDD:294696	69/135 (51%)
		SLC12	651..1068	CDD:281515	131/438 (30%)
Slc12a3	XP_017168114.1	2a30	57..1008	CDD:273347	404/1066 (38%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E2759_KOG2083
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG57166
OrthoDB	1	1.010	-	-		D135130at33208
OrthoFinder	1	1.000	-	-		FOG0001536
OrthoInspector	1	1.000	-	-		mtm8718
orthoMCL	1	0.900	-	-		OOG6_101360
Panther	1	1.100	-	-	O	PTHR11827
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	10	9.790