Protein Alignment NKCC and Slc12a2

DIOPT Version :10

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: NP_033220.2
• Gene: Slc12a2 / 20496
• MGIID: 101924
• Length: 1206
• Species: Mus musculus
• Alignment Length: 1148
• Identity: 455/1148 - (39%)
• Similarity: 664/1148 - (57%)
• Gaps: 158/1148 - (13%)

  Fly     4 RFQISKVNGTANAGYDGEARSAESDQTDAA---HPLPPTSNENHLHPKASGETGENRRSSRLSFR 65
            ||:::.|:..|::       ||:...:|||   ...|..|.:|      .|:|..:..||..|..
Mouse   134 RFRVNFVDPAASS-------SADDSLSDAAGVGGDGPNVSFQN------GGDTVLSEGSSLHSGG 185

  Fly    66 GFGN-------------FLRKSDAERKFSLAQLTKESLPRLDNYRISM----RNLKRPSIGELQG 113
            |.|:             :||        :....|.:::||:|:||.:.    ..|.|||:.||..
Mouse   186 GSGHHQQYYYDTHTNTYYLR--------TFGHNTMDAVPRIDHYRHTAAQLGEKLLRPSLAELHD 242

  Fly   114 EAVDQSITIPEPEPEATG----------------------GHIKLGWIVGVLIPCLLNIWGVMLF 156
            |.        |.||...|                      |.:|.|||.|||:.|:|||||||||
Mouse   243 EL--------EKEPFEDGFANGEESTPTRDAVVAYTAESKGVVKFGWIKGVLVRCMLNIWGVMLF 299

  Fly   157 LRLSWVVAESGILQSLIIITISAVVCVITTLSLSAISTNGEVKGGGVYFIISRSLGPEFGASVGV 221
            :||||:|.::||..|:::|.::.||..||.||.|||:|||.|:|||.|::||||||||||.::|:
Mouse   300 IRLSWIVGQAGIGLSVVVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGL 364

  Fly   222 VFAFANAVSASMNTIGFCESLNVLLKNNDLKIVDNGINDIRIVGSITVLVLILICCVGMEWETKA 286
            :|||||||:.:|..:||.|::..|||.:.:.::|. ||||||:|:|||::|:.|...|||||.||
Mouse   365 IFAFANAVAVAMYVVGFAETVVELLKEHSILMIDE-INDIRIIGAITVVILLGISVAGMEWEAKA 428

  Fly   287 QNFLIVTIVLAIFNFLIGAAIGPQGNEEQISRGFVGFSWATLKENFGSDYRYAEGVNHDFFSVFA 351
            |..|:|.::|||.:|:||..|   ..|.:..:||.|:......||||.|:|..|    .||||||
Mouse   429 QIVLLVILLLAIADFVIGTFI---SLESKKPKGFFGYKSEIFNENFGPDFREEE----TFFSVFA 486

  Fly   352 IFFPSVTGIQAGANICGDLKDAGAAIPKGTFWSLLISMSSYALFVLFAGGAAVRDASG-----IP 411
            ||||:.|||.|||||.|||.|..:||||||..::||:...|....:..|...||||:|     |.
Mouse   487 IFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTVVYIGIAVSVGSCVVRDATGNVNDTIT 551

  Fly   412 ADLVNGTIVSSELP-----CMATGNCTWGLFNSYEMMQEMSLWGPLIYAGCFAATLSTALTNLLS 471
            .:|.|.|..:.:|.     | .:..|::||.|::::|..:|.:.|||.||.|:||||:||.:|:|
Mouse   552 TELTNCTSAACKLNFDFSYC-ESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVS 615

  Fly   472 VPRLVQALGIDQIYPGLIFFSKPYGKHGEPYRGYVLTFFITTGFLLIGELNLIAPLISTFYLASY 536
            .|::.|||..|.|||....|:|.|||:.||.|||:|||.|..||:||.|||:|||:||.|:||||
Mouse   616 APKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASY 680

  Fly   537 ALINFCTFHAAFVKPLGWRPTFKYYNAWLSLFGFAMCVAIMFLINYVAAIITFGIIFALYLVVMY 601
            |||||..|||:..|..||||.|||||.|:||.|..:|..:||:||:.||::|:.|:..||:.|.|
Mouse   681 ALINFSVFHASLAKSPGWRPAFKYYNMWISLIGAILCCIVMFVINWWAALLTYVIVLGLYIYVTY 745

  Fly   602 RKPEANWGSTTQAQQYKAALMAVHRLQNVSDHVKNYHPQVLVLSGDPKTRPPLVDFGYLLTKNNS 666
            :||:.||||:|||..|.:||....||..|.|||||:.||.||::|.|.:||.|:...:..|||..
Mouse   746 KKPDVNWGSSTQALTYLSALQHSIRLSGVEDHVKNFRPQCLVMTGSPNSRPALLHLVHDFTKNVG 810

  Fly   667 LMFVANII--PVRVGYKNRQHLVKDGQKYLDARKIKAFYNVIDGFSLEDGINALTKSTGFGKMSP 729
            ||...::.  |.|...|.........|::|...|:||||..:....|.:|...|.::.|.|:|.|
Mouse   811 LMICGHVHMGPRRQAMKEMSIDQARYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKP 875

  Fly   730 NIVLVGYKPDWNRCRKEEVESYFSILYNAFSQRMGVALLRLPNGLDFSELSSEVTLPANGMGHMH 794
            |.:::|:|.||.:....:|:.|.::.::||..:.||.::||..|||.|.|..:
Mouse   876 NTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQFGVVVIRLKEGLDISHLQGQ------------ 928

  Fly   795 TANAHGFTNELMPAANAASELLHIDSNLNLASMDSPNSSFTMPQPAPMPNMQRNSRSYKVTSSDE 859
                    .||:.:...:.....:..|::.:.....::.             ::|....:|..||
Mouse   929 --------EELLSSQEKSPGTKDVVVNVDYSKKSDQDTC-------------KSSGEKSITQKDE 972

  Fly   860 ---------PAVTYHTKGGSDIP-----QNLLDAMTIFTRKQPKGTIDVFWLYDDGGLTILLPYI 910
                     |.:...:| |..:|     |.||:|.|.|.:||.|.||||:||:||||||:|:||:
Mouse   973 EEDGKTPTQPLLKKESK-GPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYL 1036

  Fly   911 ISMRSHWQNSKLRVFAMCHGKDE--EQEEKSMASLLTKFRIKYSELIMLKGVSEQPRADTVLKHK 973
            ::.:..|::.|:|||  ..||..  :.:.::||:||:||||.:|::::|..::.:|:.:.::...
Mouse  1037 LTTKKKWKDCKIRVF--IGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFD 1099

  Fly   974 RLIEPFRRGARNE-------------FGITDDELQSMSEKTNRQLRIHELVVKHSSNASLVVMSL 1025
            .:|||:|....::             :.|||:||:....||.||:|::||:.:|||.|:::||||
Mouse  1100 DMIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSL 1164

  Fly  1026 PMPRKEAISAPLYMSWLEMLTSDMKCPVALARGNQTPVLTLYS 1068
            |:.||.|:|:.|||:|||.|:.|:. ||.|.|||...|||.||
Mouse  1165 PVARKGAVSSALYMAWLEALSKDLP-PVLLVRGNHQSVLTFYS 1206

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	AA_permease_2	85..1068	CDD:459263	432/1049 (41%)
Slc12a2	NP_033220.2	PRK07764	<3..74	CDD:236090
		RFXV motif 1. /evidence=ECO:0000269|PubMed:14563843	78..81
		RFXV motif 2. /evidence=ECO:0000269|PubMed:14563843	134..137		2/2 (100%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	144..188		15/56 (27%)
		2a30	210..1206	CDD:273347	432/1049 (41%)
		Scissor helix. /evidence=ECO:0000250|UniProtKB:P55011	755..772		7/16 (44%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	954..987		5/45 (11%)