Protein Alignment NKCC and kcc-2

DIOPT Version :10

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: NP_001255279.2
• Gene: kcc-2 / 177370
• WormBaseID: WBGene00019205
• Length: 1129
• Species: Caenorhabditis elegans
• Alignment Length: 1268
• Identity: 300/1268 - (23%)
• Similarity: 501/1268 - (39%)
• Gaps: 346/1268 - (27%)

  Fly     4 RFQISKV--NGTANAGYDGEARSAESDQTDAAHPLPPTSNENHLH---PKASGETGENRRSSRLS 63
            ||::..|  .|...||..| |..:.||...|.:........|..|   |:.:..|..|..:||.|
 Worm     5 RFRVQSVADGGGGGAGSVG-ALDSVSDSNLAVNSATKKEITNKAHDFEPEPTSSTSTNTVTSRRS 68

  Fly    64 FRGFGNFLRKSDAERKFSLAQLT------KESLPRLDNYR-ISMRNLKRPSIGELQG-------- 113
            ..|.|:.|  ..|.:|.....:.      .|.:|..:.:. :::......:.|:..|
 Worm    69 SGGSGSGL--GPASKKPPNMHINIPTDEGAERIPLCEQHSPLALYEQDYDTQGQKIGTMLRKLSV 131

  Fly   114 -EAVDQSITIPEPEPEATGGHIKLGWIVGVLIPCLLNIWGVMLFLRLSWVVAESGILQSLIIITI 177
             .|.:.:....:.:|:|...  |:|.|:||.:|||.||:||:.|:||:|::..:|:.|:..::..
 Worm   132 YNATEATSVEADEKPKAAAA--KMGTIMGVFLPCLQNIFGVLFFIRLAWIIGTAGVFQAFFVVLT 194

  Fly   178 SAVVCVITTLSLSAISTNGEVKGGGVYFIISRSLGPEFGASVGVVFAFANAVSASMNTIGFCESL 242
            ...|..:|::|||||:|||.|..||.|::|||:||||.|.:||::|.....::|||...|..|.|
 Worm   195 CVSVTFLTSISLSAIATNGVVPSGGPYYMISRNLGPELGGAVGILFYLGTTIAASMYITGAIEIL 259

  Fly   243 NVLLKNNDLKIVDNGINDIRIVGSITVLVLILICCVGMEWETKAQNFLIVTIVLAIFNFLIGAAI 307
             :|......|:.|:..::.|::|::.:|:|.||...|:::..:....|::.::|.|.:.::|..:
 Worm   260 -LLYIYPQAKLFDDIYHNFRVLGTVLLLILGLIVMAGVKFVNRCALPLVIVVILCILSAILGVFV 323

  Fly   308 GPQGN------------------------------------------------------------ 312
            ...|:
 Worm   324 RYDGSDSLKFCMVGDRPVDLSTYFEKTQVQPNCTADGLQDLFCSTNGTCDHYYDRMKNIKVWKSS 388

  Fly   313 ------EEQISRG-----FVGFSW-----------------ATLKENFGSDYRYAEGVNHDFFSV 349
                  ||:..:|     |....|                 ..|.......|.|||.|. :|..:
 Worm   389 GMPAIREERAIKGIASGVFFDNLWPKYLRAGEVLSKDRKDKGDLNRGGHPSYIYAESVT-NFMIL 452

  Fly   350 FAIFFPSVTGIQAGANICGDLKDAGAAIPKGTFWSLLISMSSYALFVLFAGGAA----VRDASGI 410
            ..:||||.|||.||:|..|:|:||..:||.||..:...|...|.|.|:..|.:.    :||..|.
 Worm   453 VGVFFPSATGIMAGSNRSGNLRDAAKSIPLGTLAAQNFSSFIYLLGVVLFGASVSEMFIRDKYGR 517

  Fly   411 PADLVNGTIVSSELPCMATGNCTWGLFNSYEMMQEMSLWGPLIYAGCFAATLSTALTNLLSVPRL 475
            .|   .|.::.||:        :|.             :..:|..|||.:|....:.:|...|||
 Worm   518 SA---MGKLIISEI--------SWP-------------FPQVILFGCFMSTAGAGMQSLTGAPRL 558

  Fly   476 VQALGIDQIYPGLIFFSKPYGK---HGEPYRGYVLTFFITTGFLLIGELNLIAPLISTFYLASYA 537
            :||:..|.:.|    |.||:.|   .|||.|..:||..|....:||..:..|..||:.|:|..|.
 Worm   559 LQAIAADDVLP----FLKPFRKMDSRGEPIRAILLTLAICECGILIAVIENITALITQFFLMCYL 619

  Fly   538 LINFCTFHAAFVKPLGWRPTFKYYNAWLSLFGFAMCVAIMFLINYVAAIITFGIIFALYLVVMYR 602
            .:|......:.:|..||||.|:|::..||:.|..:|||:||:..:..|:....|...:|..:.|.
 Worm   620 GVNAACALQSLLKSPGWRPGFRYFHWSLSMIGAILCVAVMFISAWHFALFAIIIGAGVYKYIEYA 684

  Fly   603 KPEANWGSTTQAQQYKAALMAVHRLQNVSDHVKNYHPQVLVLSGDPKTRPP--LVDFGYLLTKNN 665
            ..|..||...:.....||..|:..|.:...|.:|:.||:|||:.|.::...  ::.|...|....
 Worm   685 GAEKEWGDGLRGLGLSAARFALLNLDDKPQHSRNWRPQLLVLAPDVESANTNGILSFVSQLKAGK 749

  Fly   666 SLMFVANIIPVRV--GYKNRQHLVKDGQKYLDARKIKAFYNVIDGFSLEDGINALTKSTGFGKMS 728
            .|..||:.:....  .|...|.:.:..:..:...|||.|.:|:...::.:||:.|.:::|.|.|.
 Worm   750 GLTLVAHCMEGEYADNYLKAQAVQEKLKAVVKKNKIKGFCDVLVTSNVIEGISCLVQTSGLGGMR 814

  Fly   729 PNIVLVGYKPDWNRCRKEEVESYFSILYNAFSQRMGVALLRLPNGLDFSELSSEVTLPANGMGHM 793
            .|.|::.:..||...::..|.:.|.....|.|  .....:.:|.   ::|     ..||||.
 Worm   815 HNTVVLSWPNDWKAEQEWVVANKFVSAIRAIS--AAKCAIMVPK---YAE-----KFPANGT--- 866

  Fly   794 HTANAHGFTNELMPAANAASELLHIDSNLNLASMDSPNSSFTMPQPAPMPNMQRNSRSYKVTSSD 858
                                                                       ||:
 Worm   867 -----------------------------------------------------------KVS--- 869

  Fly   859 EPAVTYHTKGGSDIPQNLLDAMTIFTRKQPKGTIDVFWLYDDGGLTILLPYIISMRSHWQNSKLR 923
                                           |.|||:|:..||||.:|||:::.....|:|:.:|
 Worm   870 -------------------------------GFIDVWWVVHDGGLLMLLPFLLRQHKTWKNTTVR 903

  Fly   924 VFAMCHGKDEEQEEKS-MASLLTKFRIKYSELIM------------------------LKGVSEQ 963
            :||:...:|...:.|: :...|...||..:..::                        ||.:::.
 Worm   904 LFAIAQMEDNNVQMKTDLEKFLYHLRIDAAVNVIEMTDSDISDYTYERTMKMEERNQYLKNLNKS 968

  Fly   964 PRADTVLKHKRLIEPFRRGAR--------------------------NEFG----------ITDD 992
            .|...:..|..::...|:.:|                          :|.|          .:||
 Worm   969 DRDKDIQNHLEIVTRERKLSRINEEAPAVVPEQRNLEVVDEEQEDGKSENGSAKIEHKGVRFSDD 1033

  Fly   993 E-----------LQSMSEKTNRQ-----------LRIHELVVKHSSNASLVVMSLPMPRKEAISA 1035
            |           |:...|:..|:           ::::||:.:.||:|.||.::||.| .:|.|.
 Worm  1034 EDSKEVKVGNGTLERDREERQRKRRYNVHKMHTAVKLNELMRQKSSDAQLVFVNLPGP-PDADSD 1097

  Fly  1036 PLYMSWLEMLTSDMKCPVALARGNQTPVLTLYS 1068
            ..||.::|.||..:. .|.|.||....|:|:||
 Worm  1098 SYYMDFIEALTEGLD-RVLLVRGTGAEVVTIYS 1129

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	AA_permease_2	85..1068	CDD:459263	274/1180 (23%)
kcc-2	NP_001255279.2	AA_permease_2	144..1129	CDD:459263	269/1124 (24%)