Protein Alignment NKCC and Slc12a6

DIOPT Version :10

• Sequence 1: NP_730938.1
• Gene: NKCC / 40663
• FlyBaseID: FBgn0051547
• Length: 1068
• Species: Drosophila melanogaster
• Sequence 2: NP_598410.2
• Gene: Slc12a6 / 107723
• MGIID: 2135960
• Length: 1150
• Species: Mus musculus
• Alignment Length: 1241
• Identity: 311/1241 - (25%)
• Similarity: 519/1241 - (41%)
• Gaps: 329/1241 - (26%)

  Fly     7 ISKVNGTANAGYDGEARSAESDQTDAAHPLPPTSNENHLHPKASGETGENRRSSRLSFRGFGNFL 71
            :|:::| |.......|....||:|.....:....::|.:..:.|....:..:.:|.::....|:
Mouse    60 MSELSG-ATTSLATVALDPSSDRTSNPQDVTEDPSQNSITGEHSQLLDDGHKKARNAYLNNSNY- 122

  Fly    72 RKSDA--ERKFSLAQLTKESLPRLDNYRISMRNLKRPSIGELQGEAVDQSITIPEPEPEATGGHI 134
            .:.|.  ::..:|.:...::.|::.:....|.|....:.|..:.|.. ::||..:.:|..:.
Mouse   123 EEGDEYFDKNLALFEEEMDTRPKVSSLLNRMANYTNLTQGAKEHEEA-ENITEGKKKPTKSP--- 183

  Fly   135 KLGWIVGVLIPCLLNIWGVMLFLRLSWVVAESGILQSLIIITISAVVCVITTLSLSAISTNGEVK 199
            ::|..:||.:|||.||:||:|||||:|||..:||||:..|:.|.....::|.:|:|||:|||.|.
Mouse   184 QMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGILQAFAIVLICCCCTMLTAISMSAIATNGVVP 248

  Fly   200 GGGVYFIISRSLGPEFGASVGVVFAFANAVSASMNTIGFCESLNV-------LLKNND-LKIVDN 256
            .||.||:|||:||||||.:||:.|......:|:|..:|..|...|       :.:::| ||....
Mouse   249 AGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFRSDDALKESAA 313

  Fly   257 GINDIRIVGSITVLVLILICCVGMEWETK-AQNFL--IVTIVLAIFNFLIGAAIGPQ-------G 311
            .:|::|:.|:..:::::|:..:|:.:..| |..||  ::..:|||:...|.::..|.       |
Mouse   314 MLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLG 378

  Fly   312 NEEQISR------------------------------------------------GFVGFSWATL 328
            |....||                                                |..|.:...:
Mouse   379 NRTLSSRHLDICSKTKEVDNMTVPSKLWGFFCNSSQFFNATCDEYFVHNNVISIQGIPGLASGII 443

  Fly   329 KENFGSDYRYAEG----------------VNHD---------FFSVFAIFFPSVTGIQAGANICG 368
            .||..|:| ..:|                :||:         |..:..|||||||||.||:|..|
Mouse   444 TENLWSNY-LPKGEIIEKPSAKSSDVLGNLNHEYVLADITTSFTLLVGIFFPSVTGIMAGSNRSG 507

  Fly   369 DLKDAGAAIPKGTFWSLL----ISMSSYALFVLFAGGAAVRDASGIPADLVNGTIVSSELPCMAT 429
            |||||..:||.||..::|    :.:|:..||.....|..:||..|   |.|.|.:|        .
Mouse   508 DLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFG---DAVKGNLV--------V 561

  Fly   430 GNCTWGLFNSYEMMQEMSLWGPLIYAGCFAATLSTALTNLLSVPRLVQALGIDQIYPGLIFFSKP 494
            |..:|           .|.|  :|..|.|.:|....|.:|...|||:||:..|.|.|    |.:.
Mouse   562 GTLSW-----------PSPW--VIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIP----FLRV 609

  Fly   495 YG---KHGEPYRGYVLTFFITTGFLLIGELNLIAPLISTFYLASYALINFCTFHAAFVKPLGWRP 556
            :|   .:|||....:||..|....:||..|:|:||::|.|:|..|..:|........::...|||
Mouse   610 FGHSKANGEPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRP 674

  Fly   557 TFKYYNAWLSLFGFAMCVAIMFLINYVAAIITFGIIFALYLVVMYRKPEANWGSTTQAQQYKAAL 621
            .|:||:..||..|.::|:|:||:.::..||:...|...:|..:.|:..|..||...:.....||.
Mouse   675 RFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAAR 739

  Fly   622 MAVHRLQNVSDHVKNYHPQVLV---LSGDPKTR-PPLVDFGYLLTKNNSLMFVANIIPVRVG--- 679
            .|:.||:....|.||:.||:||   |..|...: |.|:.|...|.....|..|.::|   ||
Mouse   740 FALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVI---VGNFL 801

  Fly   680 --YKNRQHLVKDGQKYLDARKIKAFYNVIDGFSLEDGINALTKSTGFGKMSPNIVLVGYKPDWNR 742
              |.:.....:..:..::|.|:|.|..::....|::||:.|.:|.|.|.|..|.|::|:...|
Mouse   802 ENYGDALAAEQTIKHLMEAEKVKGFCQLVVAAKLKEGISHLIQSCGLGGMKHNTVVMGWPNGW-- 864

  Fly   743 CRKEEVESYFSILYNAFSQRMGVALLRLPNGLDFSELSSEVTLPANGMGHMHTANAHGFTNELMP 807
             |:.|         :|.:.:..:..:|:                        |..||
Mouse   865 -RQSE---------DARAWKTFIGTVRV------------------------TTAAH-------- 887

  Fly   808 AANAASELLHIDSNLNLASMDSPNSSFTMPQPAPMPNMQRNSRSYKVTSSDEPAVTYHTKGGSDI 872
                            ||.:.:.|.||                                     .
Mouse   888 ----------------LALLVAKNVSF-------------------------------------F 899

  Fly   873 PQNLLDAMTIFTRKQPKGTIDVFWLYDDGGLTILLPYIISMRSHWQNSKLRVFAMCHGKDEE-QE 936
            |.|:        .:..:|.|||:|:..|||:.:|||:::.....|:...:|:|.:...:|.. |.
Mouse   900 PSNV--------EQFSEGNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQM 956

  Fly   937 EKSMASLLTKFRIK-----------------YSELIMLKGVSEQPRADTVLKHKRLIEPFR-RGA 983
            :|.:|:.|...||:                 |...:|::..|:      :|:|.||.:..| |.|
Mouse   957 KKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMMEQRSQ------MLRHMRLSKTERDREA 1015

  Fly   984 -----RNEF-------GITDDELQSMSEKT----------------------------------- 1001
                 ||..       ...|:|.::..||.
Mouse  1016 QLVKDRNSMLRLTSIGSDEDEETETYQEKVHMTWTKDKYMASRGQKVKSMEGFQDLLNMRPDQSN 1080

  Fly  1002 ----NRQLRIHELVVKHSSNASLVVMSLPMPRKEAISAPLYMSWLEMLTSDMKCPVALARGNQTP 1062
                :..::::|::|..|..|.||::::|.|.:.......||.:||:||..:: .|.|.||..:.
Mouse  1081 VRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLE-RVLLVRGGGSE 1144

  Fly  1063 VLTLYS 1068
            |:|:||
Mouse  1145 VITIYS 1150

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
NKCC	NP_730938.1	AA_permease_2	85..1068	CDD:459263	296/1159 (26%)
Slc12a6	NP_598410.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..108		9/48 (19%)
		2a30	109..1150	CDD:273347	300/1189 (25%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	161..181		5/20 (25%)
		Scissor helix. /evidence=ECO:0000250|UniProtKB:Q9UHW9	682..691		3/8 (38%)